Sequence Analysis and Prokaryotic Expression of the H and N Gene of Canine Distemper Virus Lederle Strain

doi:10.7668/hbnxb.2007.04.014

Abstract

Abstract: The haemagglutinin protein gene (H) and the nucleocapsid protein gene (N) were amplified from the genomic RNA of canine distemper virus Lederle strain by RT-PCR. The full length of the H fragment and the N fragment was 1911 bp and 1707 bp respectively. Sequence analysis showed that the H and N gene had an over 95% identity with vaccine strains such as Onderstepoort strain and CDV3 strain, while only 90%-95% with wild isolates. A 996 bp H fragment and a 562 bp N fragment were amplified and inserted into the prokaryotic expression vector pET-32a for induced expression. The size of the recombinant fusion proteins was 57 kD (H) and 37 kD (N) respectively. The recombinant fusion proteins showed positive reaction with anti-CDV canine serum by Western blotting.

Key words: Canine distemper virus, H gene, N gene, Sequence analysis, Prokaryotic expression

CLC Number:

S852.65+5

YANG Ying-li, YANG Bing, LAI Ping-an, WANG Jin-luo, XU Fu-zhou. Sequence Analysis and Prokaryotic Expression of the H and N Gene of Canine Distemper Virus Lederle Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 59-62. doi: 10.7668/hbnxb.2007.04.014.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2007.04.014

http://www.hbnxb.net/EN/Y2007/V22/I4/59

[1]	ZHAO Jie, LIU Hongquan, ZHAO Yun, YANG Kai, FU Yongbin, GU Yuzhang, SUN Lijing, HU Mengyun, LI Hui, ZHANG Yingjun. Composition and Distribution of Wheat VRN Genes and Their Relationship with Vernalization in the Northern China Winter Wheat Region [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 62-67.
[2]	XIAO Shikui, LI Fang, ZHANG Wenting, LÜ Shufang, SHI Guoan, WU Jiang, FAN Bingyou. *Cloning and Prokaryotic Expression of ACS* Gene from Paeonia lactiflora Pall.** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 36-44.
[3]	WANG Ya'nan, LIANG Yanyan, YAN Wenpei, ZHANG Yinping, LI Qiang, WU Xiuju. Cloning and Prokaryotic Expression Analysis of AhOMT1 Gene from Asarum heterotropoides [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 62-70.
[4]	LIAN Kaiqi, JI Shuang, ZHOU Lingling, SHI Zhiqi, WANG Fei, ZHANG Yuanchen. Cloning and Prokaryotic Expression of a clip-type Serine Protease Gene from Mythimna separate [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 195-201.
[5]	FAN Huifen, SUN Tianjie, SU Weihua, XIAO Fuming, ZHANG Jie, WANG Dongmei. Preparation of GmWRKY50 Protein Antibody and Analysis of Its Protein Level Expression [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 28-34.
[6]	LONG Yanxi, LUO Yanru, ZHU Zhenghang, LIAO Zhiyi, WANG Lan. Research on Protein Content and Glutelin Gene Expression in Tetroploid Rice [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 78-87.
[7]	JIN Fengmei, XUE Jun, SUN Haibo, HAO Zhiyu. *Molecular Detection and Partial Genomic Sequence Analysis of Tomato mosaic virus* in Tianjin Based on Small RNA Sequencing** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 176-183.
[8]	JIANG Nan, ZHENG Qianqian, LI Qianwen, TU Jian, SONG Xiangjun, SHAO Ying, LIU Hongmei, QI Kezong. Prokaryotic Expression Vector Construction,Expression,Purification and Identification of the APEC Virulence Gene ygeG [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 184-190.
[9]	YU Tianfei, XIE Pengyu, SUN Wanshu, LI Jing, YIN Haichang, LI Ming, YU Zhidan. *Prokaryotic Expression of Variable Domain of Monoclonal Antibody Against Goose parvovirus* NS1 Protein** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 211-215.
[10]	XU Huan, DUAN Shengwen, FENG Xiangyuan, ZHENG Ke, YANG Qi, WANG Qiming, CHENG Lifeng, PENG Yuande. High Level Expression of Pectate Lyase Gene from Microbiology for Ramie Degumming and Its Bioinformatics Analysis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 18-23.
[11]	LEI Qidong, SUN Xudong, XU Huini. *Cloning and Prokaryotic Expression of Arabidopsis AtTCP4* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 24-28.
[12]	LI Moxiao, BIAN Zhe, ZHOU Qihui, LIU Yuwei, GONG Xiaodong, GU Shouqin, HAN Jianmin. Analysis of the Gene Structure,Prokaryotic Expression and Expression Pattern of StRTG2 in Setosphaeria turcica [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 191-196.
[13]	WANG Haibo, LI Furong, YANG Jincui, GAO Yong, GUO Junyun. Cloning and Prokaryotic Expression of Phosphoglucomutase Genes cPGM and pPGM from Jatropha curcas [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 23-30.
[14]	LIU Qing, SUN Tingzhao, GAI Shupeng, ZHANG Yuxi, LIU Chunying. Characteristic and Expression Analysis of a Transcription Factor PsZFP1 in Tree Peony [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 67-73.
[15]	AERZUGULI Maimaitituerxun, ZHANG Zijun, ZHANG Weihong. *Complete Mitochondrial Genome Sequence of Oxyloma wujiaquensis* Li, Guo & Zhang, 2017(Gastropoda：Succineidae)** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 230-238.

Please choose a citation manager

Content to export