The Clone and Sequence of Bovine BMP-15 Gene cDNA

doi:10.7668/hbnxb.2007.04.015

Abstract

Abstract: BMP-15 (Bone Morphogenetic Protein-15) gene has important role in the development and differentiation of follicle and is specialized expressed in mammalian ovaries. In order to lay the theoretical foundations for further study on BMP-15 in bovine reproduction, cDNA sequence of bovine BMP-15 was cloned and analyzed. The specific primers according to conserver sequence of the other animals was designed to amplify bovine BMP-15 cDNA with RT-PCR The purified RT-PCR product was cloned into PMD18-T vector. The results showed that the bovine BMP-15 belonged to the family of bone morphogenetic protein because of an opening reading frame (ORF). The ORF contained 1185 base pairs which encoded 394 amino acid pre-pro-peptide and the pre-pro-peptide contained the BMP consensus sequence of six invariantly spaced cysteines residues. Compared with the sequence of sheep, pig, human, mouse and the ORF homology were 98.5%, 87.6%, 74.0%, 69.4% respectively.

Key words: Bovine, Bone Morphogenetic Protein-15, RT-PCR, Cloning, Sequence analysis

CLC Number:

S823.03

QI Yun-xia, WANG Feng, LIU Yong-bin, TIAN Lei, DONG Wen-jie, DA Lai, TIAN Chun-ying, RONG Wei-heng. The Clone and Sequence of Bovine BMP-15 Gene cDNA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 63-66. doi: 10.7668/hbnxb.2007.04.015.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2007.04.015

http://www.hbnxb.net/EN/Y2007/V22/I4/63

References

[1] Meler M F, Mabie P C, Zhang D M, et al. Bone morphogenetic protein in the nervus system[J]. Trends Neuro Sci, 1997, 20: 309.
[2] Zhang H, Bradley A. Mice deficient for BMP2 are nonviable and have defects in amino/chorion and cardiac development[J]. Development, 1996, 122: 2977-2986.
[3] Winner G, Blessing M, Labosky P A, et al. Bone morphogenetic protein 4 is required for mesoderm formation and patterning in the mouse[J]. Genes Dev, 1995, 9: 2105-2166.
[4] Dudley A T, Lyons K M, Robertson E J. A requirement for bone morphogenetic protein-7 during development of the mammalian kidney and eye[J]. Genes Dev, 1995, 9: 2795-2807.
[5] Souza C J, MacDougal C, Campbell B K, et al. The Booroola(FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type IB (BMP-IB) gene[J]. Endocrinol, 2001, 169: 1-6.
[6] Mulsant P, Lecerf F, Fabre S, et al. Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booloola Merino ewes[J]. Proc Natl Acad Sci USA, 2001, 98: 5104-5109.
[7] Yan C, Wang P, DeMayo J, et al. Synergistic roles of bone morphogenetic protein 15 and growth differentiation factor 9 in ovarian function[J]. Mol Endocrinol, 2001, 15 (6): 854-866.
[8] Juengel J L, Hudson N L, Heath D A, et al. Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep[J]. Biol Reprod, 2002, 67(6): 1777-1789.
[9] Otsuka F, Shimasaki S. A negative feedback system between oocyte bone morphogenetic protein 15 and growth differentiation factor 9 in ovarian function[J]. Mol Endocrinol, 2001, 15(6): 854-866.
[10] Lan Z G, Ju P, Xu X, et al. GCNF-dependent repression of BMP-15 and GDF-9 mediates gamete regulation of female fertility[J]. EMBO J, 2003, 22(16): 4070-4080.
[11] Patricia Ducy, Gerard Karsenty. The family of bone morphogenetic proteins[J]. Kidney International, 2000, 57: 2207-2214.
[12] 侯振平, 印遇龙, 黄瑞林, 等. 猪骨形成蛋白15基因编码区序列的克隆及测序研究[J]. 广西农业生物科学, 2006, 25(2): 91-95.
[13] Dube J L, Wang P, Elvin J, et al. The bone morphogenetic protein 15 gene is X-linked and expressed in oocytes[J]. Molecular Endocrinology, 1998, 12: 1809-1817.
[14] Otsuka F, Shimasaki S. A novel function of bone morphogenetic protein-15 in the pituitary: selective synthesis and secretion of FSH by gonadotropes[J]. Endocrinology, 2002, 143: 4938-4941.

[1]	JIN Yifeng, GAO Yansong, WANG Qi, WANG Mengmeng, ZHAO Di, XIONG Yi, CHEN Yang. *Cloning of SnRK2.4* Gene and It's Response to Abiotic Stress in Kentucky Bluegrass** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 9-15.
[2]	ZHANG Dongmei, FENG Yayan, XIU Zhijun, YANG Chunfang, DU Meie, LI Dezhou, ZHANG Xiaoyu. Cloning and Bioinformatics Analysis of Resistant Gene StWRKY11 Induced by Sodium Silicate in Potato by Rhizoctonia solani [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 194-203.
[3]	ZHENG Yusi, WANG Pei, GUO Ying, BAI Lirong, YU Dahui, ZHAO Sen. Molecular Cloning and Expression Analysis of PfCLEC17A in Pearl Oyster Pinctada fucata [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 230-238.
[4]	XIAO Shikui, LI Fang, ZHANG Wenting, LÜ Shufang, SHI Guoan, WU Jiang, FAN Bingyou. *Cloning and Prokaryotic Expression of ACS* Gene from Paeonia lactiflora Pall.** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 36-44.
[5]	HOU Ruize, HOU Yuxiang, XU Xiaoyong, LI Xuan, LI Meilan. Cloning and Expression Analysis of CYP Homologous Genes in Brassica rapa ssp. chinensis [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 45-51.
[6]	FENG Liting, ZHANG Jianfeng, CHI Shengqi. Establishment of a Multiplex RT-PCR Assay for Detection of Four Sweet Potato RNA Viruses [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 206-211.
[7]	LIANG Peng, ZHANG Wen, FENG Dengzhen, QIANG Hao, RONG Xuan, MENG Ke. Screening Candidate Genes Related to Meat Quality Traits in Sheep Based on Transcriptome [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 220-231.
[8]	WANG Ya'nan, LIANG Yanyan, YAN Wenpei, ZHANG Yinping, LI Qiang, WU Xiuju. Cloning and Prokaryotic Expression Analysis of AhOMT1 Gene from Asarum heterotropoides [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 62-70.
[9]	ZHANG Jinyu, XU Xinjuan, CHAO Maoni, ZHANG Xiaohong, WU Xiangyuan, GAO Jitao, HUANG Zhongwen. *Cloning and Expression Analysis of GmZAT12* Gene in Soybean** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 1-7.
[10]	JIA Zhiqiang, XU Yunyu, GAO Xue, TAO Hongzheng, CHEN Zengmin, LIU Yating, LI Yongzhong. *Cloning,Subcellular Positioning of Pepper CaWRKY30* Transcription Factor, and Its Expression Analysis by Tomato spotted wilt orthotospovirus Infected** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 186-192.
[11]	TANG Zhongli, LU Xiaonan, ZHAO Rui, LIU Qinghua, LI Meilan, LEI Fengjin, XU Xiaoyong. *Identification of Enzyme Genes Related to Carotenoid Synthesis and Cloning Analysis of PSY1* and LCYE2 in Yellow Peeled Zucchini** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 60-67.
[12]	LI Ban, LÜ Ying, YANG Mingxuan, SONG Ting, YU Fang, LIU Zhiwen. *Effects of Saline-alkali Stress on Physiology and Molecular Mechanism of Brassica napus* L.** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 86-93.
[13]	WEI Yarui, ZHAO Shuliang, CHENG Xiaohua, YAN Qi, LIU Na, ZHANG Yuxing. Effect and Mechanism of the Disease Resistance to Pear Black Spot Induced by Salicylic Acid in Yali [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 183-191.
[14]	JING Fanli, ZHANG Peipei, MIAO Yongping, CHEN Tao, LIU Yuan, YANG Delong. *Cloning and Expression Analysis of TaSPP* Gene in Wheat** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 28-34.
[15]	ZHAO Huiying, YANG Guangkai, GAO Yan, ZHANG Xiaojun, HAO Yanyan. *Cloning of Gene MdSGR2* from Apple and Construction of Plant Overexpression Vector** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 42-48.

Please choose a citation manager

Content to export