Cloning and Sequence Analysis of Interferon- cDNA of Hezuo Pig

doi:10.3969/j.issn.1000-7091.2013.04.006

Abstract

Abstract: IFN- gene was cloned by RT- PCR，the cDNA was cloned and sequenced which structure of its en- coding protein were predicted by using DNAStar software．Sequence analysis results showed that the IFN- gene ORF was 501 bp and encoded 166 amino acids，including a signal peptide of 23 amino acids(aa) at the N terminal． Relative molecular weight of the encoding protein was 19． 418 5 kDa，and the isoelectric point (pI) was 9． 54． There were 9 phosphorylation sites including 7 Sers，1 Thr and 1 Tyr．In addition，2 N- glycosylation sites were found at Asn about 39th and 106th in the line．Sense mutation and nonsense mutation in small protein or polypeptide have large influence on the analysis of system evolution and percent identity，so it should be multianalysis on nucleotide and amine acid．IFN- and plateau and hypoxia induced body to produce NO．It could inhibit parasitic disease ef- fectively．

Key words: Hezuo pig, IFN-, Cloning, Sequence analysis

CLC Number:

S828
Q78

LI Fu-qiang, ZHANG Xiao-li, MA Xiao-jun, LUO Xiu-gang, YUE Yan, JIANG Li-fei, WANG En-li, SHI Shuai-feng. Cloning and Sequence Analysis of Interferon- cDNA of Hezuo Pig[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 31-36. doi: 10.3969/j.issn.1000-7091.2013.04.006.

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