Molecular Identification and Expression Analysis of a T- DNA Insertional Mutant in Rice

doi:10.3969/j.issn.1000-7091.2013.04.007

Abstract

Abstract: Mutants play an important role in rice functional genome research．A T- DNA insertion line was ana- lyzed and identified in this study．The results showed that the T- DNA line was a homozygous insertion of OsFCA genes，and the heading- date of T- DNA insertion mutant was later than that of wild type．The RT- PCR results showed that the T- DNA insertion line was a loss- of- function mutant of OsFCA genes．In addition，the expression analysis found that Hd1 and Hd3a displayed higher expression levels in wild type in the short day condition than that of the mutant，especially in the dark time，indicating that OsFCA can positively regulate the expression of gene Hd1 and Hd3a under short- day condition．

Key words: T- DNA insertion mutagenesis, Heading date, OsFCA

CLC Number:

YI Zhi-ben, LIANG Xiao-hong. Molecular Identification and Expression Analysis of a T- DNA Insertional Mutant in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 37-40. doi: 10.3969/j.issn.1000-7091.2013.04.007.

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