Abstract: One WRKY type transcription factor gene designated as TaWRKY46 was identified in a root subtractive cDNA library in which the differential expressed genes responding to the low-Pi stress were enriched.Based on the cDNA sequence of TaWRKY46,this gene was cloned in wheat cultivar Shixin828.The cDNA of TaWRKY46 was 872 bp in length,with an open reading frame of 669 bp and an encoded polypeptide of 222 aa.TaWRKY46 contains a conserved WRKY motif and a C2H2 conserved domain.Phylogenetic analysis displays a high similarity between TaWRKY46 and HvWRKY34,a WRKY transcription family gene in barley,suggesting them to be possibly derived from a same ancestor.Compared with those in sufficient-P(2 mmol/L Pi) condition,the transcripts of TaWRKY46 in roots under the deficient-P(20 μmol/L Pi) condition were dramatically increased,suggesting that TaWRKY46 gene was involved in the response to low P stress in the plants.In the meantime,TaWRKY46 was also obviously responding to the deficiencies of nitrogen,potassium,zinc,and calcium,as well as to be markedly up-regulated by other abiotic stresses,such as drought,salinity,and abscisic acid(ABA).Therefore,TaWRKY46 has potential roles in regulating plant adaptation to nutrition deficiencies and other abiotic stress,such as drought and salinity in wheat.

Key words: Wheat(Triticum aestivum L.), TaWRKY46, Gene cloning, Expression, Abiotic stress

CLC Number: 

• S512.1