Study on Genetic Transformation of Castor （Ricinus communis L. ） Using Cotyledonary Node from Mature Seeds

doi:10.3969/j.issn.1000-7091.2012.04.021

Abstract

Abstract: In order to gain positive strains of transgenic using pre-built RNAi expression vector infection castor receptor material. In this study,several factors affecting transformation rate were studied by Agrobacterium mediated in Tong bi No. 5 castor. The results showed that ： cotyledonary node of castor were rather sensitive to kanamycin, and corresponding concentration was 250 mg/L. The pre-cuhure time of 2 - 3 days, the bacterial concentration of OD600=0.8 was the best inoeulum density and 10 minutes was the best inoculation time,co-culture for 3 days were bene- ficial to improve transformation frequency. The PCR and Southern blot analysis proved that the target gene was suc- essfully integrated into the genome of castor.

Key words: Castor, Cotyledonary node, Genetic transformation

CLC Number:

S565.6

LIU Peng, ZHANG Li-jun, ZHANG Chun-lan, HUANG Feng-lan, LI Guo-rui, CHEN Yong-sheng. Study on Genetic Transformation of Castor （Ricinus communis L. ） Using Cotyledonary Node from Mature Seeds[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 112-117. doi: 10.3969/j.issn.1000-7091.2012.04.021.

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References

[1] 王关林方宏筠.植物基因工程[M].北京：科学出版社,2002.8.

[2] Birch R G. Plant transformation:problems and strategies for practical application [J]. Ann Rev Plant Physiol Plant Mol Biol, 1997,48:297 - 326.

[3] Reddy K R K,Rao G P,Bahadur B. In vitro morphogenesis from seedling explants and callus cultures of castor (Rici- nus communis L. )[J]. Phytomorph, 1987,37:337- 340.

[4] Reddy K R K, Bahadur B. Adventitious bud formation from leaf cultures of castor (Ricinus communis L. ) [J]. Curr Sci,1989,58:152 - 154.
[5] Sarvesh A, Ram Rao D M, Reddy T P. Callus initiation and plantlet regeneration from epicotyl and cotyledonary explants of castor ( Ricinus communis L. ) [J]. Adv Plant Sci,1992,5:124 - 128.

[6] Ahn Y J,Chen G Q. In Vitro Regeneration of Castor ( Ric- inus communis L. ) Using Cotyledon Explants [J]. Hort- sience,2008,43 ( 1 ) :215 - 219.

[7] Alam I, Sharmin S A, Mondal S C, et al. In vitro micro- propagation through cotyledonary node culture of castor bean (Ricinus communis L. ) [J]. Australian Journal of Crop Science,2010,4(2) :81 -84.

[8] Sujatha M,Sailaja M. Stable genetic transformation of cas- tor (Ricinus communis L. ) via agrobacterium tumefa- ciens-mediated gene transfer using embryo axes from ma- ture seeds[J]. Plant Cell Rep,2005,23:803 - 810.

[9] Malathi B, Ramesh S, Rao KV, et al. Agrobacterium-me- diated genetic transformation and production of semiloop- er resistant transgenenic castor(R icinus comm unis L. ) [J]. E uphytica, 2006,147:441 - 449.

[10] Sailaja M, Tarakeswari M, Sujatha M. Stable genetic transformation of castor ( Ricinus communis L. ) via par- ticle gun-mediated gene transfer using embryo axes from mature seeds [J]. Plant Cell Rep, 2008,27 : 1509 - 1519.

[11] Sujatha M, Reddy T P, Mahasi M J. Role of biotechno- logical interventions in the improvement of castor( Rici- nus communis L. ) and Jatropha curcas L. [J]. Biotech- nology Advances,2008,26:424 - 435.

[12] Sangwan R S, Bourgeois Y, Sangwan N B S. Genetic transformation of Arobidopsis thaliana zygotic embryos and identification of critical parameters influencing transformation efficiency [J]. Mul Gen Genet, 1991, 230:475 - 485.

[13] 《农杆菌介导的植物转基因研究中抑菌剂的应用现状》[J].福建轻纺,2009(5).

[14] 《蓖麻的组织培养》[J].西北师范大学学报：自然科学版,2010(1).

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