Genetic Diversity of Maize Inbred Lines Revealed by SSR in Northeast Provinces

doi:10.3321/j.issn:1000-7091.2006.z1.006

Abstract

Abstract: Simple sequence repeats (SSRs) were used to detect genetic diversity among 66 maize (Zea mays L.) inbred lines, which were widely used in northeast provinces of China. Seventy selected SSR primers gave stable profiles amplified in samples of 66 inbred lines. Seventy primers produced 273 polymorphic amplified fragments. The average number of allele per SSR locus was 3.9 which varied from 2 to 7. The polymorphism information content (PIC) for the SSR loci varied from 0.121 to 0.814 with an average of 0.584. Genetic similarities among the 66 inbred lines ranged from 0.62 to 0.93. The cluster analysis showed that the inbred lines could be classified into 5 distinct clusters. The results were consistent with the groups based on the available pedigree. Most of the parents used in maize commercial hybrids came from opposite clusters.

Key words: Simple sequence repeats, Maize inbred line, Polymorphism information content (PIC), Genetic diversity

XIAO Mu-ji, LI Ming-shun, LI Xin-hai, ZHANG Shi-huang. Genetic Diversity of Maize Inbred Lines Revealed by SSR in Northeast Provinces[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 23-27. doi: 10.3321/j.issn:1000-7091.2006.z1.006.

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