Hypoxia-induced Expression of Vitreoscilla Hemoglobin Gene in Escherichia coli

doi:10.3321/j.issn:1000-7091.2006.z1.005

Abstract

Abstract: The 570 bp Vitreoscilla hemoglobin gene (vgb) was obtained by synthesizing the native promoter of vgb and jointing it with vgb structural gene. The recombinant vector pUC-LV constructed by cloning the gene into pUC18 plasmid was transformed into Escherichia coli. After ten hours of induction under low dissolved oxygen conditions, the density of recombinant strain increased 67.4% compaired with that of control. The results of SDS-PAGE and carbon monoxide (CO) difference spectrum analysis indicated that the recombinant strain expressed Vitreoscilla hemoglobin (VHb) having biological activity.

Key words: Vitreoscilla hemoglobin gene (vgb), Native promoter, Escherichia coli, Hypoxia induction

CLC Number:

Q786

ZHANG Feng, ZHAO Xiao-yu, ZHOU Yan-fen. Hypoxia-induced Expression of Vitreoscilla Hemoglobin Gene in Escherichia coli[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 20-22. doi: 10.3321/j.issn:1000-7091.2006.z1.005.

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