Use of Random Amplified Polymorphic DNA(RAPD) Markers for the Identification of Genetic Diversity in Chinese Cabbage

doi:10.3321/j.issn:1000-7091.2006.03.020

Abstract

Abstract: Genetic diversity of sixty-four germplasm accessions of Chinese cabbage(B.pekinensis) with a turnip(B.rapa) and a Xiaobaicai(B.chinensis L.) were analyzed using RAPD(Random Amplified Polymorphic DNA)marker.Sixteen primers with sharp and polymorphic bands selected from four sets of primer(OPE,OPF,OPG,OPH) that have eighty primers were selected to analyze materials.100 amplified bands were produced.Among them,72 bands were polymorphic.The percentage of polymorphic was 72%.The results can show the typical bands of turnip,Xiaobaicai and Chinese cabbage.These three typical bands can be used as RAPD markers for identification of these three species.Nei-Li similarities computed by 72 polymorphic DNA markers were used in unweighted pair group method of arithmetic mean(UPGMA) cluster analyses.All materials were clustered into groups corresponding to the eight types by similarity(0.721),i.e.,six Chinese cabbage types which include 64 accessions of Chinese Cabbage,turnip type and Xiaobaicai type.

Key words: Chinese Cabbage, Germplasm, Genetic Diversity

CLC Number:

S634.1

SONG Shun-hua, ZHENG Xiao-ying, XU Jia-bing, YU Yang-jun. Use of Random Amplified Polymorphic DNA(RAPD) Markers for the Identification of Genetic Diversity in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 86-90. doi: 10.3321/j.issn:1000-7091.2006.03.020.

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