Study on Methods for Ploidy DetermiNa+tion in Regenerated Plant from UnpolliNa+ted Ovules of Cucurbita pepo

doi:10.3321/j.issn:1000-7091.2004.03.020

Abstract

Abstract: Chromosome counting and chloroplast counting in guard cells were used in the ploidy determina-tion of the regenerated plants from unpollinated ovules of cucubita pepo.The results indicated the guard cell chloroplast numbers in haploid，diploid and tetraploid were (4.1510.37)，(8.0510.76 ) and(16.OS士0.69) respectively;The ratio of them was approximately 1:2:4，which showed chloroplast counting was a reliable and practicable method in ploidy determination. In addition, the size of guard cell of haploid, diploid and tetraploid were ( 20.31士3.38 ) hem，(30.80土2.19)p.m, (41.78土1.03 ) p.m respectively，and the ratio of them was ap-proximately 1:1.5:2，which showed the method of counting the size of guard cell was also a practicable method in ploidy determination of regenerated plants that we can refer to.

Key words: Cucurbita , Unpollinated ovules, Regenerated plants, Methods for ploidy determination

CLC Number:

S642.6

GUO Yong-qiang, WANG Jian-she, ZHANG Hui-ling, WEN Cai-xiang, LI Hai-zhen, CAI Min-hua. Study on Methods for Ploidy DetermiNa⁺tion in Regenerated Plant from UnpolliNa⁺ted Ovules of Cucurbita pepo[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(3): 80-83. doi: 10.3321/j.issn:1000-7091.2004.03.020.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2004.03.020

http://www.hbnxb.net/EN/Y2004/V19/I3/80

[1]	LIU Ze-fa, MIN Zi-yang, SUN Xiao-wu, CHENG Juan, HU Yi-hong. Study on Induction and Characterization of Tetraploid Plants in Pumpkin [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 125-129.
[2]	YAN Lei-yan, ZHANG Yan-yu, MA Kai-hui, SONG Hui, WANG Yu-hong. *Multiple PCR Detection of Fusarium oxysporum* and Fusarium solani in Cucurbitaceous Plants** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 44-47.
[3]	ZHANG Guo-yu, ZHANG Fan, JIANG Li-gang, ZHAI Wei-bo, LI Hai-zhen. *EST-SSR Markers Development from Cucurbitaand Their Use in Purity Testing of F₁Hybrid Seed* [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 97-101.
[4]	ZHOU Jun-guo,HU Hui-ling,ZHAO Run-zhou,WU Hang,XIE Zhen-wei. Effect of Cucurbita moschata Hybrids Rootstock on the Growth of Grafted Cucumber Seedling [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 117-120.
[5]	CAO Li-xia, CHEN Gui-lin, DUN Hui-xia, ZHANG Ke. Effect of Phosphorus Deficiency on Root Growth and Root Exudates of Cucurbita ficif olia B. [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 164-169.
[6]	LI Nan, ZHANG Hai-ying, CHEN Nian-lai, WANG Yong-jian, XU Yong, GONG Guo-yi, GUO Shao-gui. Comparison of Versatility and Polymorphism of SSRs in Three Cucurbitaceae [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 110-114.
[7]	LIU Shuan-tao, ZHAO Zhi-zhong, SUN Xiao-lei, LU Jin-dong. Critical Factors Influence in vitro Plantlet Production of Unfertilized Ovule Culture in Summer Squash(Cucurbita pepo L.) [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(2): 96-100.
[8]	Ouyang Xinxing, Xu Yong, Zhang Haiying, Wang Yongjian. Studies on Early-mature and Classification in Squash (Cucurbita pepo L.) Using Principal Component Analysis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(2): 125-128.
[9]	Li Chunhong, Meng Xiangqi, Jiang Youyi. Anther Culture and Ploidy Identification of Regenerated Plants of Corn [J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(2): 64-68.
[10]	Bai Jianrong, Du Zhuming, Zhou Guoyu. Characters of Regenerated Plants of Male Sterile Lines and Hybrids in Seteria italica [J]. ACTA AGRICULTURAE BOREALI-SINICA, 1992, 7(2): 51-54.

Please choose a citation manager

Content to export