Abstract: Some factors influencing on corn anther culture and the ploidy identification of regenerated plants of corn were studied.The results indicated that genotype was the main influencing factor.The response of anther culture in the unstable line was not as good as in the hybrid,but was better than in the self- line.Both N6 and Zheng14 media were suitable for corn anther culture.The liquid medium was better than the solid medium.The inoculating period of anther was either the mid or the late stages of the uninucleate microspore.The late stage was better than the mid stage.Calli could not be induced at the early stage of the uninucleate microspore.The difference in length of the stoma guard cell reached the highly significant level between diploid and haploid regenerated plants.These plants with stoma guard cells shorter than 29 μm were haploid ones,and those with stoma quard cells longer than 29μm were diploid or polyploid ones.The accuracy reached 95%.The method for measurement of the length of stoma guard cell could be used to identify ploidy.

Key words: Corn, Anther culture, Ploidy, Regenerated plants, Stoma quard cell