Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2004, Vol. 19 ›› Issue (2): 5-9. doi: 10.3321/j.issn:1000-7091.2004.02.002

Special Issue: Cucumber；

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Optimization of SSR Condition on Cucumber

GE Feng-wei1, ZHANG Hai-ying2, CHEN Qing-jun3, WANG Yong-jian2, XU Yong2   

  1. 1. Department of Horticulture, Xinjiang Agriculture University, Urumchi 830052, China;
    2. National Engineering Research Center for Vegetable,Beijing 100089,China;
    3. China Agricultural University, Beijing 100094, China
  • Received:2003-12-23 Published:2004-06-28

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Abstract: In this study,we optimizatied SSR condition on cucumber. In 25 μL PCR reaction,0.2 mmol/L Mg2+;0.2 mmol/L dNTP;lU Taq polymerase,30 ng primer 5 ng/μL DNA are the best. It will have high resolution in polyacylamide gels electrophoresis.

Key words: Cucumber(Cucumis sativus L), SSR, Agarose gels, Polyacrylamide gels

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Cite this article

GE Feng-wei, ZHANG Hai-ying, CHEN Qing-jun, WANG Yong-jian, XU Yong. Optimization of SSR Condition on Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(2): 5-9. doi: 10.3321/j.issn:1000-7091.2004.02.002.

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