An Simplified Procedure of Polyacrylamide Gel Electrophoresis Applicable to the Study of the Gliadin of Wheat Seed

doi:10.3321/j.issn:1000-7091.2003.02.012

Abstract

Abstract: Based on the standard procedure of polyacrylamide geLelectrophotesis set by ISTA for the analysis of the gliadin of wheat and barley seed for the variety identification, we screened the main influence factors which would affect the electrophoresis, and tried to improve them to get the best combination.According to the results, we established an optimaLprocedure, it had the buffer system of Sodium Hydroxide Acetic Acid Glycine, and the voltage of electrophoresis should be lower at beginning, then higher for a while, and lower in the end.T his method had the properties of high resolution and stability, using less samples, and the geLcan be easily desqua-mated than the other methods.Furthermore, this method is easily to operate, more economical, and spend less time than the standard procedures, so it can be used in many laboratories to study the gliadin of wheat.

Key words: Wheat, Gliadin of seed, Polyacrylamide gel electrophotesis

CLC Number:

S512.01

GAO Ju rong, WANG Hong gang, LIU Shu bing, LI Xing feng, KONG Fan jing, CHEN Dong hua. An Simplified Procedure of Polyacrylamide Gel Electrophoresis Applicable to the Study of the Gliadin of Wheat Seed[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(2): 43-46. doi: 10.3321/j.issn:1000-7091.2003.02.012.

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References

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