Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2002, Vol. 17 ›› Issue (4): 54-58. doi: 10.3321/j.issn:1000-7091.2002.04.012

Special Issue: Wheat；

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Artificial Inoculation of Wheat Yellow Mosaic Virus to Wheat

SHANG Qiao-xia1, HAN Cheng-gui1, YU Jia-lin2, YANG Li-li1, LIU Yi2   

  1. 1. Department of Plant Pathology, China Agricultural University, Beijing 100094, China;

    2. State Key Lab for Agrobiotechnology, China Agricultural University, Beijing 100094, China
  • Received:2001-11-30 Published:2002-12-28

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Abstract: Factors affecting artificial inoculation of WYMV on susceptible wheat variety were analyzed.The result indicated that the highest incidence could be gained by using the dried diseased-leaf or fresh wheat to inoculate wheat cultivated at low temperature.The diseased-leaf should be grounded in phosphate buffer. The effective inoculation method was developed and WYMV in wheat leaves was tested by ELISA and RT-PCR.

Key words: Wheat yellow mosaic virus, Mechanical inoculation, Virus detection

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SHANG Qiao-xia, HAN Cheng-gui, YU Jia-lin, YANG Li-li, LIU Yi. Artificial Inoculation of Wheat Yellow Mosaic Virus to Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(4): 54-58. doi: 10.3321/j.issn:1000-7091.2002.04.012.

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