Quick Detection of Turnip Mosaic Virus by One-Step Multiple RT-PCR

doi:10.3969/j.issn.1000-7091.2012.03.020

Abstract

Abstract: Turnip Mosaic virus(Turnip Mosaic virus，TuMV)infects and causes damage in many crucifers and?ornamentals. Using of M-MLV and general Taq enzyme，we developed a rapid，convenience and specificity method?for detecting of TuMV by one-step multiple RT-PCR. This simple method didn't need the step of purification of total?RNA. After grinding the samples directly in extraction buffer at room temperature，the crude extract or concentrated?DNA-RNA mixture could be used as detection template. In order to ensure the accuracy of the detection，two sets of?primers were employed in this method.

Key words: TuMV, One-step, Multiple RT-PCR, Virus detection

CLC Number:

S41.30
S43

ZENG Gang, YE Yan-ying,YAN Xiao-hong, MA Rong-cai, TANG Le-chen, YAO Lei. Quick Detection of Turnip Mosaic Virus by One-Step Multiple RT-PCR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 102-107. doi: 10.3969/j.issn.1000-7091.2012.03.020.

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