Detective and Complete Sequence Analysis of Wheat Yellow Mosaic Virus from Zhumadian in Henan Province

doi:10.7668/hbnxb.2010.02.002

Abstract

Abstract: A total of 13 wheat samples from different fields in Zhumadian of Henan Province were investigated,using RT-PCR,ELISA and Western Blotting,the results showed that WYMV existed there.Complete RNA genomic cDNA of one positive isolate(WYMV-ZMD)was cloned and sequenced,and was compared with those of isolates from Huangchuan,Yangzhou,Ya'an and Japan.Sequence comparisons showed that WYMV-ZMD shared 97.1%-98.4% nucleotide sequence identity for RNA1 with the reported isolates,and 94.2%-98.2% identity for RNA2.At the amino acid level,WYMV-ZMD had 94.8%-97.8% identity for RNA1,and 94.2%-98.2% identity for RNA2,respectively.Further phylogenetic analysis of five different isolates based on RNA1 and RNA2 by MEGA 4.1 indicated that WYMV-ZMD was more closely related to WYMV-HC,and had a more distant relationship with Ya'an isolate.

Key words: Wheat yellow mosaic virus, Zhumadian isolate, Detection, Complete sequence

CLC Number:

ZHANG Zong-ying, XU Jian-mei, HAN Cheng-gui, ZHANG Zhen-chen, LI Da-wei, YU Jia-lin. Detective and Complete Sequence Analysis of Wheat Yellow Mosaic Virus from Zhumadian in Henan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 5-11. doi: 10.7668/hbnxb.2010.02.002.

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