Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2002, Vol. 17 ›› Issue (4): 49-53. doi: 10.3321/j.issn:1000-7091.2002.04.011

Special Issue: Strawberry；

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Study on Tissue Culture and Regeneration Plantlet from Strawberry Leaf

SUN Rui-fen1, LI Tian-ran2, LI Kun1, DENG Xiang-lan2, SHI Hui-qin1, ZHANG Ying-li3, JIA Li-min3   

  1. 1. Institute of Horticulture, Inner Mongolia Academy of Agricultural Sciences, Huhhot 010010, China;
    2. Faculty of Life Science, Inner Mongolia University, Huhhot 010010, China;
    3. Inner Mongolia Academy of Agricultural Sciences, Huhhot 010031, China
  • Received:2002-02-12 Published:2002-12-28

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Abstract: The medium of regeneration plantlet from shoot tip of strawberry creeping stem supplemented with different hormones was worked out.The protocol of tissue culture and rapid propagation has been developed. The influences of hormone condition, genotypes on shoot regeneration from leaf disc of strawberry were examined.By further studying, the effects of genotypes and explants on shoot induction were also examined when basal medium was MS+ B5 supplemented with 2.253 mg/L 6-BA and 1.752 mg/L IAA. An efficient and higher frequence method of direct shoot regeneration has been established. Meanwhile, a suitable receptor material of genetic transformation.

Key words: Strawberry, Shoot tip of creeping stem, Tissue culture, Rapid propagation, Leaf disc, Induction

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Cite this article

SUN Rui-fen, LI Tian-ran, LI Kun, DENG Xiang-lan, SHI Hui-qin, ZHANG Ying-li, JIA Li-min. Study on Tissue Culture and Regeneration Plantlet from Strawberry Leaf[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(4): 49-53. doi: 10.3321/j.issn:1000-7091.2002.04.011.

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