Isolation and Express Analysis of FaCO-2, a CO Homologue from Strawberry

doi:10.3969/j.issn.1000-7091.2012.04.001

Abstract

Abstract: The CO homologue, FaCO-2,was isolated from strawberry （Fragaria x ananassa cv. Huaji） with the method of PCR and RT-PCR. Its express pattern in different tissues and organs was checked with qRT-PCR. The full length of its DNA sequence is 1 882 bp and its CDS is 1 146 bp in length. FaCO-2 codes a predicted protein of 382 amino acids. The amino acid identity compared with other CO homologues is highly conserved. The protein molecular weight was 42 021.69 Da with an isoelectric point （PI） of 5.49. The real-time RT-PCR result showed that FaCO-2 mainly transcripts differ in different tissues and floral organs. It was detected its highest expression in the expanded leaf. In the four flower organs, it was only detected in sepal, while no transcription was detected in other flower organs.

Key words: Strawberry, CO homologue, Isolation, Expression

CLC Number:

LIU Yue-xue, ZOU Dong-mei, LI He, ZHANG Zhi-hong, MA Yue, DAI Hong-yan. Isolation and Express Analysis of FaCO-2, a CO Homologue from Strawberry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 1-6. doi: 10.3969/j.issn.1000-7091.2012.04.001.

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