Purity Determination of Chinese Cabbage F1 Cultivars with RAPD Markers

doi:10.3321/j.issn:1000-7091.2000.04.008

Abstract

Abstract: Randomly amplified polymorphic DNA(RAPD)markers were applied to distinguish purity of commercial F1-hybrid seeds of Chinese cabbage. DNA from two F1-hybrid cultivars, Beijing No.57 and Beijing No.106, and their parental lines were screening with 50 primers. Primer OPE-20 generated in F1-hybrid Beijing No.57 specific RAPD markers. Primer OPH-06 and OPH-07 generated in F1-hybrid Beijing No.106 specific RAPD markers. These markers enabled a clear distinction to be made between F1-hybrids and their parental lines. These primers were used in purity control of hybrid seed production of Beijing No.57 and Beijing No.106 . This result indicated the practical usefulness of RAPD markers in commercial hybrid seeds purity tests of Chinese cabbage.

Key words: Chinese cabbage, RAPD marker, Hybrid purity

SONG Shunhua, ZHENG Xiaoying. Purity Determination of Chinese Cabbage F1 Cultivars with RAPD Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2000, 15(4): 35-39. doi: 10.3321/j.issn:1000-7091.2000.04.008.

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