Identification of Main Chinese Cabbage Cultivars with Random Amplified Polymorphic DNA Markers

doi:10.3321/j.issn:1000-7091.2000.03.001

Abstract

Abstract: Twenty-one Chinese cabbage cultivars were analyzed with Random amplified polymorphic DNA(RAPD). Using 13 arbitrary 10-mer primers, we scored a total of 87 clear and repeatable different fragments for their presence/absence. 39 bands (44.8%)were polymorphic. Primer OPE-01 had the highest ratio of polymorphic, with which 15 Chinese cabbage cultivars could be distinguished. Primer OPE-01 combined with primer OPH-03 and primer OPH-12 could distinguish all of the Chinese cabbage cultivars.

Key words: Chinese cabbage, Main cultivars, RAPD markers

SONG Shun hua, ZHENG Xiao ying. Identification of Main Chinese Cabbage Cultivars with Random Amplified Polymorphic DNA Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2000, 15(3): 1-5. doi: 10.3321/j.issn:1000-7091.2000.03.001.

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