摘要： 用农杆菌介导法对苹果品种嘎拉转化FT基因进行了研究，建立了苹果离体叶片高效再生体系，在含BA mg/L +IAA 0.mg/L的MS培养基上，以离体叶片远轴面(叶背面)向上，经过14 d的暗培养获得再生不定芽和再生植株，再生率达97%。在转基因再生体系中，以头孢噻肟钠00 mg/L和卡那霉素 mg/L，作为脱菌培养和选择培养的条件，经过感染携带拟南芥FT基因的农杆菌，获得了一批转FT基因的植株，经PCR检测，目的基因已经整合到苹果基因组中。

关键词: 苹果, 农杆菌, FT基因, 遗传转化

Abstract: In this study,high-frequency regeneration system was founded in leaf blade explants of apple cultiva Gala,which were transformated by Agrobacteria-mediated with FT gene. During cultivating,it was important to keep the anticous surface of leaf not contact with the surface of MS cultivation,after 14d dark cultivation,the regeneration buds and shoots were obtainted in MS (the MS cultivation contained: 5 mg/ L BA,0.5 mg/ L IAA),in this transformation system,the regeneration frequecy was up to 97%. Using cefotaxime sodium (500 mg/ L) and Kanamycin (5 mg/ L) as the in-hibitor of Agrobacteria overgrowth and selection pressure,the explants were infected with Agrobacteria that containted FT gene,then achieved FT gene transgenic plants. The results of PCR amplification suggested that FT gene has integrated into apple plants genomes.

Key words: Apple, Agrobacterium tumef aciens, FT gene, Genetic transformation

中图分类号: 

• Q785