巨须裂腹鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析

doi:10.7668/hbnxb.20192209

摘要/Abstract

摘要： 为了了解巨须裂腹鱼的生化遗传特性，丰富巨须裂腹鱼种质资源研究的内容，通过聚丙烯胺凝胶垂直板电泳对巨须裂腹鱼5种组织（心脏、眼睛晶状体、肌肉、肝脏和肾脏）中的乳酸脱氢酶（LDH）和苹果酸脱氢酶（MDH）进行了电泳分析。结果表明：所测样本鱼心脏LDH酶带数为6~7条、眼睛晶状体LDH酶带数为6~10条、肌肉LDH酶带数为5~7条、肝脏LDH酶带数为8~11条和肾脏中的LDH酶带数为8~10条；LDH-C基因只在肝脏中特异性表达。心脏MDH酶带数为4~8条、眼睛晶状体MDH酶带数为2~4条、肌肉MDH酶带数为3~4条、肝脏MDH酶带数为4~5条和肾脏MDH酶带数为4~7条。5种组织中LDH在肝脏中表达的酶带数最多，肝脏MDH酶带着色最深，表达活性程度最强。巨须裂腹鱼的LDH和MDH同工酶系统具有组织特异性和个体差异性。组织特异性主要体现在LDH和MDH酶带数目和表达活性程度不同两方面，与各组织执行特定生理功能密切相关。巨须裂腹鱼同工酶个体差异性可能是其漫长的进化过程中为了能适应特殊生境的需要。研究结果可从生化遗传角度为巨须裂腹鱼制定种质标准和资源保护利用提供一定理论依据。

关键词: 巨须裂腹鱼, 乳酸脱氢酶, 苹果酸脱氢酶, 组织特异性, 个体差异性

Abstract: This paper was to learn about the biochemical heredity characteristics and to enrich the research data about the germplasm resources of Schizothorax macropogon. Lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)isozymes from five tissues(heart, crystalline lens, muscle, liver and kidney)of Schizothorax macropogon were preliminarily studied by using the vertical polyacrylamide gel electrophoresis. The results showed that:the band numbers of LDH isozymes expressed in heart was 6-7, in crystalline lens 6-10, in muscle 5-7, in liver 8-11 and in kidney, 8-10 respectively, and the band numbers of MDH isozymes expressed in heart was 4-8, in crystalline lens 2-4, in muscle 3-4, in liver 4-5 and in kidney 4-7. LDH-C gene only specificly expressed in liver. The band numbers expressed in liver are the largest in five different tissues. MDH bands expressed in liver presented the darkest and showed the most degree of isozyme activity. LDH isozymes and MDH isozymes showed tissue specificity and individual variations. The tissue specificity mainly reflected in band numbers and degree of isozyme activity. There were correlations between tissue specificity and its specificly physiological functions. In order to adapt the unique habitant, the isozymes of Schizothorax macropogon showed individual variations. The research results can provide theoretical basis for making germplasm standards and the conservation and utilization for Schizothorax macropogon from the biochemical genetics level.

Key words: Schizothorax macropogon, LDH isozymes, MDH isozymes, Tissue specificity, Individual variations

中图分类号:

S917.4

张涛, 张林, 周剑光, 甘金华, 何力. 巨须裂腹鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析[J]. 华北农学报, 2021, 36(S1): 390-396. doi: 10.7668/hbnxb.20192209.

ZHANG Tao, ZHANG Lin, ZHOU Jianguang, GAN Jinhua, HE Li. Electrophoretic Analysis on Lactate Dehydrogenase(LDH)and Malate Dehydrogenase(MDH)Isozymes in Five Different Tissues in Schizothorax macropogon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 390-396. doi: 10.7668/hbnxb.20192209.

http://www.hbnxb.net/CN/Y2021/V36/IS1/390

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