不同盐度、碱度、pH值对瓦氏雅罗鱼精子活力及其受精率的影响

doi:10.7668/hbnxb.20192551

华北农学报 ›› 2021, Vol. 36 ›› Issue (S1): 381-389. doi: 10.7668/hbnxb.20192551

所属专题： 盐碱胁迫；水产；

• 畜牧·水产·兽医 • 上一篇下一篇

不同盐度、碱度、pH值对瓦氏雅罗鱼精子活力及其受精率的影响

李乾^1,2, 梁利群², 艾克达·热合曼^2,3, 孙博², 张立民², 董志国¹, 常玉梅²

1. 江苏海洋大学, 江苏省海洋生物资源与环境重点实验室, 江苏连云港 222005;
2. 中国水产科学研究院黑龙江水产研究所, 淡水鱼类育种国家地方联合工程实验室, 农业农村部淡水水产生物技术与遗传育种重点实验室, 中国水产科学研究院特殊生境鱼类种质特性与抗逆育种重点实验室, 黑龙江哈尔滨 150070;
3. 大连海洋大学水产与生命学院, 辽宁大连 116023

收稿日期:2021-07-29 出版日期:2021-12-28
通讯作者: 常玉梅(1978-),女,黑龙江哈尔滨人,研究员,博士,主要从事鱼类分子生理研究。
作者简介:李乾(1996-),男,河南南阳人,在读硕士,主要从事鱼类分子生理研究。
基金资助:
国家重点研发计划（2019YFD0900405）；中国水产科学研究院基本科研业务费（2017HY-ZD0404；2020TD22）

Effects of Different Salinity,Alkalinity and pH Value on Sperm Motility and Fertilization Rate of Amur ide

LI Qian^1,2, LIANG Liqun², AI Keda^2,3, SUN Bo², ZHANG Limin², DONG Zhiguo¹, CHANG Yumei²

1. Jiangsu Key Laboratory of Marine Biological Resources and Environment, Jiangsu Ocean University, Lianyungang 222005, China;
2. National & Local United Engineering Laboratory for Freshwater Fish Breeding, Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish stress Resistance Breeding and Germplasm Characteristics on Special Habitat, Chinese Academy of Fishery Sciences, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
3. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China

Received:2021-07-29 Published:2021-12-28

摘要/Abstract

摘要： 为了掌握瓦氏雅罗鱼精子活力及其受精率的最适盐度、碱度和pH的范围，通过设置不同NaCl盐度（1‰，2‰，3‰，4‰，5‰，6‰，7‰，8‰）、NaHCO₃碱度（10，20，30，40，50，60，70 mmol/L）、pH值（5.0，5.5，6.0，6.5，7.0，7.5，8.0）梯度范围的溶液及淡水对照组（盐度0、碱度0、pH值6.67）激发达里诺尔湖瓦氏雅罗鱼精子活力，观察了不同处理组精子状态，并对其激烈运动时间、快速运动时间及寿命进行了测定，同时测定了受精卵在不同盐度、碱度和pH溶液中的受精率。结果表明：盐度在0~4‰，瓦氏雅罗鱼精子激烈运动、快速运动时间及寿命呈逐渐上升趋势，4‰达到最长；碱度在0~50 mmol/L，精子激烈运动、快速运动时间及寿命随碱度增加而延长，50 mmol/L达到最大；pH值5.0~7.0，精子激烈运动、快速运动时间及寿命随pH值的升高而延长，pH值7.0达到最大；受精率分别在盐度4‰、碱度30 mmol/L、pH值6.5达到最高，不同盐度和碱度组的受精率显著低于淡水对照组。综上，基于对不同盐度、碱度和pH值对瓦氏雅罗鱼精子活力及其受精率的分析结果，建议在开展瓦氏雅罗鱼全人工繁殖及杂交育种过程中，尽量选择盐度在0~4‰，碱度为0~30 mmol/L，pH值6.0~7.0的盐碱水体或淡水中进行，可以获得较高的受精率及苗种成活率。

关键词: 瓦氏雅罗鱼, 精子活力, 受精率, 盐度, 碱度, pH值

Abstract: In order to determine the optimum range of salinity, alkalinity and pH value of sperm motility and fertilization rate of Amur ide. Different NaCl salinity(1‰, 2‰, 3‰, 4‰, 5‰, 6‰, 7‰, 8‰), NaHCO₃ alkalinity(10, 20, 30, 40, 50, 60, 70 mmol/L)and pH value(5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0)were set to stimulate the sperm motility of the Darinor Lake Amur ide.The sperm status of different treatment groups was observed, and the intense movement time, fast movement time and life span of the sperm were measured. Meanwhile, the fertilization rate in different salinity, alkalinity and pH solutions was determined.The results showed as follows. When the salinity ranged from 0 to 4‰, the sperm intense movement, fast movement time and life span increased gradually, and reached the maximum at 4. When the alkalinity was 0-50 mmol/L, the sperm intense movement, fast movement time and life span were prolonged with the increase of alkalinity, and reached the maximum at 50 mmol/L. In the pH value 5.0-7.0, the sperm intense movement, fast movement time and life span increased with the increase of pH, pH value 7.0 reached the maximum. The fertilization rates reached the highest when the salinity was 4‰, the basicity was 30 mmol/L and the pH value was 6.5, respectively. The fertilization rates in different salinity and alkalinity groups were significantly lower than those in the control group. In conclusion, based on the analysis results of different salinity, alkalinity and pH on sperm motility and fertilization rate of Amur ide, it was suggested to select saline-alkali water or fresh water with salinity of 0-4‰, alkalinity of 0-30 mmol/L and pH of 6.0-7.0 in the process of total artificial reproduction and cross breeding of Amur ide. It can obtain higher fertilization rate and seedling survival rate.

Key words: Amur ide, Sperm motility, Rate of fertilization, Salinity, Alkalinity, pH value

中图分类号:

S965

李乾, 梁利群, 艾克达·热合曼, 孙博, 张立民, 董志国, 常玉梅. 不同盐度、碱度、pH值对瓦氏雅罗鱼精子活力及其受精率的影响[J]. 华北农学报, 2021, 36(S1): 381-389. doi: 10.7668/hbnxb.20192551.

LI Qian, LIANG Liqun, AI Keda, SUN Bo, ZHANG Limin, DONG Zhiguo, CHANG Yumei. Effects of Different Salinity,Alkalinity and pH Value on Sperm Motility and Fertilization Rate of Amur ide[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 381-389. doi: 10.7668/hbnxb.20192551.

http://www.hbnxb.net/CN/Y2021/V36/IS1/381

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Xu W,Geng L W,Li C T,Jin G X,Liu X Y. The artificial propagation,embryonic development and saline-alkali tolerant experiment of Barbus capito[J]. Journal of Fisheries of China,2011,35(2):255-260.

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不同盐度、碱度、pH值对瓦氏雅罗鱼精子活力及其受精率的影响

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