荣昌猪、长白猪先天免疫候选基因筛选

doi:10.7668/hbnxb.20192398

摘要/Abstract

摘要： 旨在探究影响荣昌猪和长白猪机体先天免疫功能的候选基因。以新生荣昌猪、长白猪胸腺为研究对象，利用RNA-seq分析荣昌猪、长白猪胸腺组织中的差异表达基因，同时对差异表达基因进行GO和KEGG富集分析，并通过qRT-PCR验证RNA-seq结果的准确性。结果表明：在新生荣昌猪和长白猪胸腺中，差异倍数在2倍以上的基因共421个，其中，荣昌猪相对长白猪上调的有231个，下调的有190个。GO富集分析发现，这些差异表达基因主要富集在清道夫受体活性、G蛋白偶联受体结合、T细胞活化、细胞分泌调节、胞吐作用调节、SNARE复合物组装调节等生物学过程。KEGG通路分析发现，这些差异表达基因主要参与细胞因子-细胞因子受体互作、cAMP信号、代谢（磷脂酶D、半胱氨酸和蛋氨酸）及一些疾病（非洲锥形病、癌症）相关信号通路。6个差异表达基因的RT-qPCR结果表明，此次RNA-seq的结果准确可靠。综合差异表达基因、GO和KEGG信号通路结果发现，TMSB15A和STAB2、SAA1和HP分别是荣昌猪和长白猪胸腺先天免疫的候选基因。

关键词: 猪, 先天免疫, RNA-seq, 候选基因, 抗病

Abstract: The aim of this study was to explore the innate immune candidate genes in Rongchang pigs and Landrace. Thymus of newborn Rongchang pigs and Landrace were used as the research objects. The RNA-seq technology was used to analyze the differentially expressed genes in the thymus of Rongchang pigs and Landrace. Then, the GO and KEGG enrichment analysis of differentially expressed genes were performed, and the qRT-PCR was used to verify the accuracy of RNA-seq. The results showed that a total of 421 differentially expressed genes were identified, including 231 up-regulated genes and 190 down-regulated genes of Rongchang pig compared to Landrace. GO enrichment analysis showed that these differentially expressed genes were mainly enriched in the biological processes such as scavenger receptor activity, G protein-coupled receptor binding, T cell activation, regulation of secretion by cell, exocytosis and SNARE complex assembly. KEGG pathway analysis showed that these differentially expressed genes were mainly involved in signal pathways such as cytokine-cytokine receptor interaction, cAMP signal, cysteine and methionine metabolism, phospholipase D signaling and some diseases(African cone disease, cancer).The RT-qPCR results of 6 differentially expressed genes showed that the results of RNA-seq were accurate and reliable. Based on the results of screening, GO and KEGG of differentially expressed genes, TMSB15A and STAB2, SAA1 and HP were candidate genes for thymus innate immunity in Rongchang pigs and Landrace, respectively.

Key words: Pig, Innate immune, RNA-seq, Candidate genes, Disease resistance

中图分类号:

S828.8+9

龙熙, 赵久刚, 柴捷, 潘红梅, 张亮, 张廷焕. 荣昌猪、长白猪先天免疫候选基因筛选[J]. 华北农学报, 2021, 36(S1): 373-380. doi: 10.7668/hbnxb.20192398.

LONG Xi, ZHAO Jiugang, CHAI Jie, PAN Hongmei, ZHANG Liang, ZHANG Tinghuan. Screening of Innate Immune Candidate Genes in Rongchang Pigs and Landrace[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 373-380. doi: 10.7668/hbnxb.20192398.

http://www.hbnxb.net/CN/Y2021/V36/IS1/373

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