摘要： 胰岛素样生长因子结合蛋白与动物的生长发育有密切的联系。为了探讨IGFBP-3基因作为绵羊屠宰性状候选基因的可能性，采用单链构象多态性结合测序方法检测592只甘肃高山细毛羊IGFBP-3基因的变异特征，并分析核苷酸变异与屠宰性状的相关性。结果表明，绵羊IGFBP-3第4外显子区检测到1个单核苷酸变异位点（g.87 A>C），A和B 2个等位基因（等位基因频率：56.76%，43.24%），表现为AA、AB和BB 3种基因型（基因型频率：29.73%，54.05%，16.22%），并且所研究的群体在该位点上处于Hardy-Weinberg平衡状态（P>0.05）。相关性结果表明，甘肃高山细毛羊IGFBP-3基因g.87 A>C处的变异对宰前活质量、热胴体质量有极显著影响（P<0.01）；对眼肌面积、GR值、嫩度有显著影响（P<0.05）。表明等位基因B的绵羊群体具有更高的屠宰率，可以作为甘肃高山细毛羊屠宰性状选育的分子标记用于生产实践中。

关键词: 甘肃高山细毛羊, IGFBP-3基因, 变异, 屠宰性状

Abstract: Insulin-like Growth Factor Binding Proteins (IGFBPs) are closely related to the growth and development of animals.In order to explore the possibility of IGFBP-3 gene as a candidate gene for sheep slaughter traits, this study used Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) combined with sequencing to detect variation characteristics of IGFBP-3 gene and to explore the correlation between nucleotide variation and slaughter traits in 592 Gansu alpine merino sheep.The results showed that one single nucleotide variant site (g.87 A>C) was detected in the fourth exon region of sheep IGFBP-3, and two alleles A and B (alle frequency:56.76% and 43.24%), showing three genotypes of AA, AB and BB (genotype frequency:29.73%, 54.05%, 16.22%), and the population studied was in the Hardy-Weinberg equilibrium state at this locus (P>0.05).The correlation results showed that the mutation at g.87 A>C of Gansu alpine merino sheep IGFBP-3 gene had a very significant effect on live weight and hot carcass weight before slaughter (P<0.01);it had a significant effect on eye muscle area, GR value and tenderness.Degree had a significant impact (P<0.05).It showed that the sheep population of allele B had a higher slaughter rate, and it could be used as a molecular marker for the selection of slaughter traits of Gansu alpine merino sheep in production practice.

Key words: Gansu alpine merino sheep, IGFBP-3 gene, Variation, Meat quality traits

中图分类号: 

• Q78

• S826