<i>STAT3</i>基因核苷酸序列变异对绵羊泌乳性状的影响

doi:10.7668/hbnxb.20195805

华北农学报 ›› 2025, Vol. 40 ›› Issue (5): 198-207. doi: 10.7668/hbnxb.20195805

所属专题： 畜牧；

• 畜牧·水产·兽医 • 上一篇下一篇

STAT3基因核苷酸序列变异对绵羊泌乳性状的影响

杨盼¹, 甄慧敏¹, 郝志云¹, 张希云², 车陇杰³, 李明娜¹, 任春燕¹, 王煊宇¹, 赵源¹, 刘圆¹, 王继卿¹^,, 王自科¹^,³^,

¹ 甘肃农业大学动物科学技术学院, 甘肃省牛羊基因改良工程实验室,甘肃省草食动物生物技术重点实验室, 甘肃兰州 730070
² 甘肃元生农牧科技有限公司, 甘肃金昌 737100
³ 甘肃省畜牧技术推广总站, 甘肃兰州 730030

收稿日期:2025-01-22 出版日期:2025-11-03
通讯作者:
王继卿(1982—),男,甘肃永昌人,研究员,博士,博士生导师,主要从事绵山羊遗传育种研究。
王自科(1977—),男,甘肃景泰人,正高级畜牧师,硕士,主要从事畜禽高效养殖技术示范与推广工作。
作者简介:
杨盼(2000—),女,甘肃榆中人,硕士,主要从事动物遗传育种与繁殖研究。
基金资助:
国家自然科学基金项目(32402728); 甘肃省基础研究创新群体项目(22JR5RA829); 甘肃省重点研发计划项目(23YFNA0009); 甘肃省第三批陇原青年英才项目

Effect of Nucleotide Sequence Variation of STAT3 on Milk Performance Traits of Sheep

YANG Pan¹, ZHEN Huimin¹, HAO Zhiyun¹, ZHANG Xiyun², CHE Longjie³, LI Mingna¹, REN Chunyan¹, WANG Xuanyu¹, ZHAO Yuan¹, LIU Yuan¹, WANG Jiqing¹^,, WANG Zike¹^,³^,

¹ College of Animal Science and Technology, Gansu Engineering Lab of Genetic Improvement in Ruminants, Gansu Key Laboratory of Herbivorous Animal Biotechnology,Gansu Agricultural University, Lanzhou 730070, China
² Gansu Yuansheng Agricultural Animal Husbandry Technology Co., Ltd., Jinchang 737100, China
³ Gansu General Station of Animal Husbandry Technology Extension, Lanzhou 730030, China

Received:2025-01-22 Published:2025-11-03

摘要/Abstract

摘要：

为探讨STAT3基因单核苷酸多态性与绵羊泌乳性状的关联性,选择414只泌乳期奶绵羊为试验对象,用RT-qPCR技术检测STAT3基因在乳腺等7个组织中的表达,用Sanger测序技术检测STAT3基因单核苷酸多态性,用五引物扩增受阻突变体系(PARMS)技术对其进行分型,并分析该基因核苷酸序列变异对绵羊泌乳性状的影响。RT-qPCR结果发现,STAT3基因在肺脏中表达量最高,肝脏和乳腺组织次之,脾脏中最低。在绵羊STAT3基因中共鉴定到c.22+537 C/T、c.22+658 C/T、c.318+126 C/G、c.417+360 G/A和c.417+395 T/C 5个新的SNPs,每个SNPs位点均检测到3种基因型。相关性分析发现,c.318+126 C/G位点处的CC型个体的乳脂率高于GG型个体,c.22+537 C/T位点处的CC型、c.22+658 C/T位点处的CC型、c.417+360 G/A位点处的GG型以及c.417+395 T/C位点处的TT型个体均具有较高的乳脂率和干物质含量。单倍型组合H1H7对乳脂率、干物质含量以及灰分含量有显著正效应,H1H2个体的日均泌乳量比H1H7提高了0.266 kg。综上,筛选到5个STAT3基因的核苷酸变异位点,可为奶绵羊泌乳性状的分子选种提供理论依据。

关键词: 奶绵羊, STAT3, 核苷酸序列变异, 泌乳性状, 基因表达

Abstract:

To explore the association between STAT3 single nucleotide polymorphism and lactation traits of sheep,414 lactating sheep were selected as test subjects,the expression of STAT3 in seven tissues including mammary gland was detected by RT-qPCR,the single nucleotide polymorphism of STAT3 was detected by sanger sequencing,STAT3 was typed by Penta-primer amplification refractory mutation system PARMS,and the effect of the nucleotide sequence variation of the gene on milk performance traits was analysed.RT-qPCR results revealed that STAT3 expression was highest in lungs,followed by liver and mammary tissues,and lowest in spleen.A total of five novel SNPs,c.22+537 C/T,c.22+658 C/T,c.318+126 C/G,c.417+360 G/A and c.417+395 T/C,were identified in the sheep STAT3,and three genotypes were detected at each SNP locus.Association analysis revealed that ewes with the genotype CC at the c.318+126 C/G locus had a higher milk fat rate than those with GG,and CC-type at the c.22+537 C/T locus,CC-type at the c.22+658 C/T locus,GG-type at the c.417+360 G/A locus,and TT-type at the c.417+395 T/C locus all had higher milk fat percentage and dry matter content.The haplotype combination H1H7 exhibited significant positive effects on milk fat percentage,dry matter content,and ash content.Additionally,the average daily milk yield of individuals carrying the haplotype combination H1H2 was increased by 0.266 kg compared to those carrying H1H7.In conclusion,the five nucleotide variation sites of STAT3 screened in this study may provide a theoretical basis for molecular selection of milk performance traits in milk sheep.

Key words: Milk sheep, STAT3, Nucleotide sequence variation, Milk performance traits, Gene expression

中图分类号:

S826

杨盼, 甄慧敏, 郝志云, 张希云, 车陇杰, 李明娜, 任春燕, 王煊宇, 赵源, 刘圆, 王继卿, 王自科. STAT3基因核苷酸序列变异对绵羊泌乳性状的影响[J]. 华北农学报, 2025, 40(5): 198-207. doi: 10.7668/hbnxb.20195805.

YANG Pan, ZHEN Huimin, HAO Zhiyun, ZHANG Xiyun, CHE Longjie, LI Mingna, REN Chunyan, WANG Xuanyu, ZHAO Yuan, LIU Yuan, WANG Jiqing, WANG Zike. Effect of Nucleotide Sequence Variation of STAT3 on Milk Performance Traits of Sheep[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 198-207. doi: 10.7668/hbnxb.20195805.

http://www.hbnxb.net/CN/Y2025/V40/I5/198

图/表 9

参考文献 37

[1]	赵丽丽, 葛武鹏, 宋宇轩, 安小鹏. 绵羊奶组成和营养特性[J]. 中国食品学报, 2022, 22(1):413-423.doi:10.16429/j.1009-7848.2022.01.044.
	Zhao L L, Ge W P, Song Y X, An X P. The composition and nutrition characteristics in sheep milk[J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22(1):413-423.
[2]	Li R N, Ma Y H, Jiang L. Review:research progress of dairy sheep milk genes[J]. Agriculture, 2022, 12(2):169.doi:10.3390/agriculture12020169.
[3]	Jiang R Y, Zhu J Y, Zhang H P, Yu Y, Dong Z X, Zhou H H, Wang X J. STAT3:key targets of growth-promoting receptor positive breast cancer[J]. Cancer Cell International, 2024, 24(1):356.doi:10.1186/s12935-024-03541-9.
[4]	Hu X Y, Li J, Fu M R, Zhao X, Wang W. The JAK/STAT signaling pathway:from bench to clinic[J]. Signal Transduction and Targeted Therapy, 2021, 6:402.doi:10.1038/s41392-021-00791-1.
[5]	Boutinaud M, Jammes H. Growth hormone increases Stat5 and Stat1 expression in lactating goat mammary gland:a specific effect compared to milking frequency[J]. Domestic Animal Endocrinology, 2004, 27(4):363-378.doi:10.1016/j.domaniend.2004.04.002.
[6]	Zhou J B, Wan F, Wang L, Peng C, Huang R Z, Peng F. STAT4 facilitates PD-L1 level via IL-12R/JAK2/STAT3 axis and predicts immunotherapy response in breast cancer[J]. MedComm, 2023, 4(6):e464.doi:10.1002/mco2.464.
[7]	Schmitt-Ney M, Doppler W, Ball R K, Groner B. β-casein gene promoter activity is regulated by the hormone-mediated relief of transcriptional repression and a MammaryGland-specific nuclear factor[J]. Molecular and Cellular Biology, 1991, 11(7):3745-3755.doi:10.1128/mcb.11.7.3745-3755.1991.
[8]	刘圆. JAK2和STAT5A对绵羊乳腺上皮细胞特性和泌乳性状的影响[D]. 兰州: 甘肃农业大学, 2024.doi:10.27025/d.cnki.ggsnu.2024.000169.
	Liu Y. Effects of JAK2 and STAT5A on characteristics of mammary epithelial cells and lactation traits in sheep[D]. Lanzhou: Gansu Agricultural University, 2024.
[9]	Khaled W T, Read E K C, Nicholson S E, Baxter F O, Brennan A J, Came P J, Sprigg N, McKenzie A N J, Watson C J. The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development[J]. Development, 2007, 134(15):2739-2750.doi:10.1242/dev.003194.
[10]	Semenzato G, Calabretto G, Teramo A, Gasparini V R, Rampazzo E, Barilà G, Zambello R. The constitutive activation of STAT3 gene and its mutations are at the crossroad between LGL leukemia and autoimmune disorders[J]. Blood Cancer Journal, 2024, 14(1):13.doi:10.1038/s41408-024-00977-0.
[11]	Chapman R S, Lourenco P C, Tonner E, Flint D J, Selbert S, Takeda K, Akira S, Clarke A R, Watson C J. Suppression of epithelial apoptosis and delayed mammary gland involution in mice with a conditional knockout of Stat3[J]. Genes & Development, 1999, 13(19):2604-2616.doi:10.1101/gad.13.19.2604.
[12]	Hughes K, Watson C J. The multifaceted role of STAT3 in mammary gland involution and breast cancer[J]. International Journal of Molecular Sciences, 2018, 19(6):1695.doi:10.3390/ijms19061695.
[13]	翟梦星. 荷斯坦奶牛BRCA2基因、STAT3基因SNPs检测及其与乳房炎和产奶性状的关联分析[D]. 杨凌: 西北农林科技大学, 2015.
	Zhai M X. Detection of SNPs of BRCA2 gene and STAT3 gene in Holstein cows and their association with mastitis and milk production traits[D]. Yangling: Northwest A&F University, 2015.
[14]	Zhou H, Hickford J G H, Fang Q. A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification[J]. Analytical Biochemistry, 2006, 354(1):159-161.doi:10.1016/j.ab.2006.03.042.
[15]	吴贤锋. 山羊STAT3和STAT5A基因SNPs、mRNA表达及甲基化对生产性能的影响[D]. 杨凌: 西北农林科技大学, 2015.
	Wu X F. Effects of SNPs and mRNA expression and methylation of STAT3 and STAT5A genes on production performance in goats[D]. Yangling: Northwest A&F University, 2015.
[16]	Paris A J, Hayer K E, Oved J H, Avgousti D C, Toulmin S A, Zepp J A, Zacharias W J, Katzen J B, Basil M C, Kremp M M, Slamowitz A R, Jayachandran S, Sivakumar A, Dai N, Wang P, Frank D B, Eisenlohr L C, Cantu E Ⅲ, Beers M F, Weitzman M D, Morrisey E E, Worthen G S. STAT3-BDNF-TrkB signalling promotes alveolar epithelial regeneration after lung injury[J]. Nature Cell Biology, 2020, 22(10):1197-1210.doi:10.1038/s41556-020-0569-x.
[17]	Khaliq M, Ko S, Liu Y Z, Wang H L, Sun Y H, Solnica-Krezel L, Shin D. STAT3 regulates liver progenitor cell-driven liver regeneration in zebrafish[J]. Gene Expression, 2018, 18(3):157-170.doi:10.3727/105221618x15242506133273.
[18]	Hardwick L J A, Davies B P, Pensa S, Burge-Rogers M, Davies C, Baptista A F, Knott R, S McCrone I, Po E, Strugnell B W, Waine K, Wood P, Khaled W T, Summers H D, Rees P, Wills J W, Hughes K. In the murine and bovine maternal mammary gland signal transducer and activator of transcription 3 is activated in clusters of epithelial cells around the day of birth[J]. Journal of Mammary Gland Biology and Neoplasia, 2024, 29(1):10.doi:10.1007/s10911-024-09561-5.
[19]	Huang H X, Ruan Y F, Li C L, Zheng H, Tang Y T, Chen Y J, He F L, Liu Y, Wu G K, Li Z H, Wang Y G, Liao Y L, Bin J P, Chen Y M. Hypoxia microenvironment preconditioning attenuated myocardial ischemia-reperfusion injury via Stc1-mediating cardiomyocyte self-protection and neutrophil polarization[J]. Advanced Science, 2025, 12(6):2411880.doi:10.1002/advs.202411880.
[20]	Song M Y, Wang C R, Yang H T, Chen Y P, Feng X J, Li B, Fan H G. P-STAT3 inhibition activates endoplasmic reticulum stress-induced splenocyte apoptosis in chronic stress[J]. Frontiers in Physiology, 2020, 11:680.doi:10.3389/fphys.2020.00680.
[21]	Xu J W, Wang M Y, Mao Y, Hu Z Y, Miao X L, Jiang F, Zhou G P. Inhibition of STAT3 alleviates LPS-induced apoptosis and inflammation in renal tubular epithelial cells by transcriptionally down-regulating TASL[J]. European Journal of Medical Research, 2024, 29(1):34.doi:10.1186/s40001-023-01610-9.
[22]	Ouyang H, Wu S C, Sung L Y, Yang S H, Yang S H, Chong K Y, Chen C M. STAT3 is an upstream regulator of granzyme G in the maternal-to-zygotic transition of mouse embryos[J]. International Journal of Molecular Sciences, 2021, 22(1):460.doi:10.3390/ijms22010460.
[23]	Sollero B P, Howard J T, Spangler M L. The impact of reducing the frequency of animals genotyped at higher density on imputation and prediction accuracies using ssGBLUP1[J]. Journal of Animal Science, 2019, 97(7):2780-2792.doi:10.1093/jas/skz147.
[24]	韩浩园, 李涛, 李世凯, 宋小雨, 李君, 哈斯, 赵金艳, 魏红芳, 权凯. GJB6和PRKAA1基因多态性及其与槐山羊产羔数的关联分析[J]. 华北农学报, 2023, 38(4):225-232.doi:10.7668/hbnxb.20193872.
	Han H Y, Li T, Li S K, Song X Y, Li J, Ha S, Zhao J Y, Wei H F, Quan K. Analysis of GJB6 and PRKAA1 genes polymorphisms and their association with lambing number in Huai goats[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(4):225-232.
[25]	Bora S K, Tessema T S, Girmay G. Genetic diversity and population structure of selected Ethiopian indigenous cattle breeds using microsatellite markers[J]. Genetics Research, 2023, 2023(1): 1106755.doi:10.1155/2023/1106755.
[26]	周步峰, 石福岳, 张建军, 何茂昌, 王耀荣, 谢文章. FSHR基因多态性及其与陇东绒山羊产双羔关联性分析[J]. 中国畜牧杂志, 2021, 57(8):67-71.doi:10.19556/j.0258-7033.20210302-04.
	Zhou B F, Shi F Y, Zhang J J, He M C, Wang Y R, Xie W Z. Polymorphism of FSHR gene and its correlation with double lambs in long Dong Cashmere goats[J]. Chinese Journal of Animal Science, 2021, 57(8):67-71.
[27]	黄志强, 王维民, 张德印, 赵源, 张煜坤, 徐丹, 杨晓斌, 马宗武, 何丽娟, 蔡有鑫, 刘晓强, 张小雪. 湖羊ANO5基因多态性及其与脂肪沉积性状的关联分析[J]. 华北农学报, 2025, 40(1):207-214.doi:10.7668/hbnxb.20194928.
	Huang Z Q, Wang W M, Zhang D Y, Zhao Y, Zhang Y K, Xu D, Yang X B, Ma Z W, He L J, Cai Y X, Liu X Q, Zhang X X. Analysis of ANO5 gene polymorphism and its association with fat deposition traits in Hu sheep[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1):207-214.
[28]	Seo S, Takayama K, Uno K, Ohi K, Hashimoto R, Nishizawa D, Ikeda K, Ozaki N, Nabeshima T, Miyamoto Y, Nitta A. Functional analysis of deep intronic SNP rs13438494 in intron 24 of PCLO gene[J]. PLoS One, 2013, 8(10):e76960.doi:10.1371/journal.pone.0076960.
[29]	Li R F, Song X W, Gao S, Peng S Y. Analysis on the interactions between the first introns and other introns in mitochondrial ribosomal protein genes[J]. Frontiers in Microbiology, 2022, 13:1091698.doi:10.3389/fmicb.2022.1091698.
[30]	Li Y F, Dang X L, Chen R, Teng Z W, Wang J Y, Li S W, Yue Y Y, Mitchell B L, Zeng Y, Yao Y G, Li M, Liu Z C, Yuan Y G, Li T, Zhang Z J, Luo X J. Cross-ancestry genome-wide association study and systems-level integrative analyses implicate new risk genes and therapeutic targets for depression[J]. Nature Human Behaviour, 2025, 9(4):806-823.doi:10.1038/s41562-024-02073-6.
[31]	Fu L Z, Crawford L, Tong A, Luu N, Tanizaki Y, Shi Y B. Sperm associated antigen 7 is activated by T3 during Xenopus tropicalis metamorphosis via a thyroid hormone response element within the first intron[J]. Development, Growth & Differentiation, 2022, 64(1):48-58.doi:10.1111/dgd.12764.
[32]	Singh O P, Mishra S, Sharma G, Sindhania A, Kaur T, Sreehari U, Das M K, Kapoor N, Gupta B. Evaluation of intron-1 of odorant-binding protein-1 of Anopheles stephensi as a marker for the identification of biological forms or putative sibling species[J]. PLoS One, 2022, 17(7):e0270760.doi:10.1371/journal.pone.0270760.
[36]	Huang J L, Yang Z M, Wang Y X, Li X S, Liu C Y, Chen J P, Qin Q W, Yang M. Association between PPAR-δ gene SNPs,haplotypes and resistances to SGIV and RGNNV in the orange-spotted grouper,Epinephelus coioides[J]. Journal of South China Agricultural University, 2023, 44(3):391-401.
[37]	Liang H, Sedillo J C, Schrodi S J, Ikeda A. Structural variants in linkage disequilibrium with GWAS-significant SNPs[J]. Heliyon, 2024, 10(11):e32053.doi:10.1016/j.heliyon.2024.e32053.
[33]	Pietrzak-Fiec'ko R, Kamelska-Sadowska A M. The comparison of nutritional value of human milk with other mammals' milk[J]. Nutrients, 2020, 12(5):1404.doi:10.3390/nu12051404.
[34]	Jia W C, Wu X F, Li X C, Xia T, Lei C Z, Chen H, Pan C Y, Lan X Y. Novel genetic variants associated with mRNA expression of signal transducer and activator of transcription 3(STAT3)gene significantly affected goat growth traits[J]. Small Ruminant Research, 2015, 129:25-36.doi:10.1016/j.smallrumres.2015.05.014.
[35]	Huang K, Dunn D W, Li W K, Wang D, Li B G. Linkage disequilibrium under polysomic inheritance[J]. Heredity, 2022, 128(1):11-20.doi:10.1038/s41437-021-00482-1.
[36]	黄健玲, 杨子敏, 王雨欣, 李鑫帅, 刘翠瑜, 陈锦鹏, 秦启伟, 杨敏. 石斑鱼PPAR-δ基因SNP位点和单倍型与抗虹彩病毒和神经坏死病毒抗性的关联[J]. 华南农业大学学报, 2023, 44(3):391-401.doi:10.7671/j.issn.1001-411X.202203022.

引物 Primer	引物序列(5'—3') Primer sequence(5'—3')	扩增区域 Application range	退火温度/℃ Annealing temperature	目的 Purpose
P1	F: CCAGAGCATTGTGAGTGAGC	第8外显子	60	SNP筛选
	R: TCTTCATGCAGCTCTGACCA
P2	F: CTTCCCCTTCTCCAGCTCAT	第9外显子	60	SNP筛选
	R: CAGCTCCACGATTCTCTCCT
P3	F: CTGCCTATTCATCCCTCCCC	第1内含子	62	SNP筛选
	R: GGTGACACGCCTTCCTTTAG
P4	F: TGACCAACAGTATAGCCGCT	第3内含子	62	SNP筛选
	R: CTACGGTGTCCAAGCCTTTG
P5	F: ACTGGCTCCTCTGTCCATTT	第4内含子	60	SNP筛选
	R: TCCCACGCCTAGAAATCTGT
P6	F: GGAAGTGGGGATGGGAAACA	第11内含子	62	SNP筛选
	R: GGAGGAAAAGACCAACCACG
P7	F: TTTGTAGGTAAGGCTGGGCA	3'非翻译区	60	SNP筛选
	R: CCAAGACCAGGTGCGTAAAG
STAT3	F: CGGCCAGCAAAGAATCACAT	编码序列	58	RT-qPCR
	R: CCACAATTCGGGCAATCTCC
β-actin	F: AGCCTTCCTTCCTGGGCATGGA	编码序列	58	RT-qPCR
	R: GGAGCGAAACTGACACCC

引物 Primer	引物序列(5'—3') Primer sequence(5'—3')	扩增区域 Application range	退火温度/℃ Annealing temperature	目的 Purpose
P1	F: CCAGAGCATTGTGAGTGAGC	第8外显子	60	SNP筛选
	R: TCTTCATGCAGCTCTGACCA
P2	F: CTTCCCCTTCTCCAGCTCAT	第9外显子	60	SNP筛选
	R: CAGCTCCACGATTCTCTCCT
P3	F: CTGCCTATTCATCCCTCCCC	第1内含子	62	SNP筛选
	R: GGTGACACGCCTTCCTTTAG
P4	F: TGACCAACAGTATAGCCGCT	第3内含子	62	SNP筛选
	R: CTACGGTGTCCAAGCCTTTG
P5	F: ACTGGCTCCTCTGTCCATTT	第4内含子	60	SNP筛选
	R: TCCCACGCCTAGAAATCTGT
P6	F: GGAAGTGGGGATGGGAAACA	第11内含子	62	SNP筛选
	R: GGAGGAAAAGACCAACCACG
P7	F: TTTGTAGGTAAGGCTGGGCA	3'非翻译区	60	SNP筛选
	R: CCAAGACCAGGTGCGTAAAG
STAT3	F: CGGCCAGCAAAGAATCACAT	编码序列	58	RT-qPCR
	R: CCACAATTCGGGCAATCTCC
β-actin	F: AGCCTTCCTTCCTGGGCATGGA	编码序列	58	RT-qPCR
	R: GGAGCGAAACTGACACCC

位点 Locus	引物序列(5'—3') Primer sequence(5'—3')	备注 Note
SNP1	CCAGAATATCTTTTGGTTGGAAGC	ASP (C)
	TCCAGAATATCTTTTGGTTGGAAGT	ASP (T)
	CTGAATCAGAGTTGCCAAGTGAAC	LSP
SNP2	AGTAATATTCCATTGTCTGTGTGTACC	ASP (C)
	TGAGTAATATTCCATTGTCTGTGTGTACT	ASP (T)
	GTGCTCAAACTTGGGAACAATTAT	LSP
SNP3	AAGGAGAAGTTACCAGCCCTTTC	ASP (C)
	AGGAGAAGTTACCAGCCCTTTG	ASP (G)
	CACATTATTATTTCAATGAACCAACC	LSP
SNP4	CATGATGTAATTAAAAGAATGCAAGAC	ASP (G)
	CATGATGTAATTAAAAGAATGCAAGAT	ASP (A)
	TCATAACTGTCTCCAGGCTCCTA	LSP
SNP5	TTAGCTTTAAACTGCTTGACTTGGA	ASP (T)
	TAGCTTTAAACTGCTTGACTTGGG	ASP (C)
	TAGCCTTTCATAACTGTCTCCAGG	LSP
通用引物1 Universal primer 1	GAAGGTGACCAAGTTCATGCT	CFP (FAM)
通用引物2 Universal primer 2	GAAGGTCGGAGTCAACGGATT	CFP (HEX)

位点 Locus	引物序列(5'—3') Primer sequence(5'—3')	备注 Note
SNP1	CCAGAATATCTTTTGGTTGGAAGC	ASP (C)
	TCCAGAATATCTTTTGGTTGGAAGT	ASP (T)
	CTGAATCAGAGTTGCCAAGTGAAC	LSP
SNP2	AGTAATATTCCATTGTCTGTGTGTACC	ASP (C)
	TGAGTAATATTCCATTGTCTGTGTGTACT	ASP (T)
	GTGCTCAAACTTGGGAACAATTAT	LSP
SNP3	AAGGAGAAGTTACCAGCCCTTTC	ASP (C)
	AGGAGAAGTTACCAGCCCTTTG	ASP (G)
	CACATTATTATTTCAATGAACCAACC	LSP
SNP4	CATGATGTAATTAAAAGAATGCAAGAC	ASP (G)
	CATGATGTAATTAAAAGAATGCAAGAT	ASP (A)
	TCATAACTGTCTCCAGGCTCCTA	LSP
SNP5	TTAGCTTTAAACTGCTTGACTTGGA	ASP (T)
	TAGCTTTAAACTGCTTGACTTGGG	ASP (C)
	TAGCCTTTCATAACTGTCTCCAGG	LSP
通用引物1 Universal primer 1	GAAGGTGACCAAGTTCATGCT	CFP (FAM)
通用引物2 Universal primer 2	GAAGGTCGGAGTCAACGGATT	CFP (HEX)

位点 Locus	群体 Populations	个体数 Number
SNP1			CC	CT	TT	C	T
	H	35	5.71	62.86	31.43	37.14	62.86	0.040 6	0.466 9	1.876 0	0.357 9
	EF	17	11.77	52.94	35.29	38.24	61.76	0.618 2	0.472 3	1.895 1	0.360 8
	F₁	193	8.29	50.78	40.93	33.68	66.32	0.057 6	0.446 7	1.807 4	0.346 9
	F₂	169	10.65	46.75	42.60	34.02	65.98	0.592 1	0.449 0	1.814 7	0.348 2
	平均值		9.11	53.33	37.56	35.77	64.23
SNP2			CC	CT	TT	C	T
	H	35	5.71	34.29	60.00	22.86	77.14	0.869 5	0.352 7	1.544 8	0.290 5
	EF	17	23.53	47.06	29.41	47.06	52.94	0.818 8	0.498 3	1.993 1	0.374 1
	F₁	193	13.47	51.30	35.23	39.12	60.88	0.285 3	0.476 3	1.909 6	0.362 9
	F₂	169	12.43	51.48	36.09	38.17	61.83	0.238 4	0.472 0	1.893 9	0.360 6
	平均值		13.79	46.03	40.18	36.80	63.20
SNP3			CC	CG	GG	C	G
	H	35	14.29	57.14	28.57	42.86	57.14	0.324 1	0.489 8	1.960 0	0.369 8
	EF	17	5.88	47.06	47.06	29.41	70.59	0.582 5	0.415 2	1.710 1	0.329 0
	F₁	193	19.17	52.33	28.50	45.34	54.66	0.438 1	0.495 7	1.982 8	0.372 9
	F₂	169	11.83	55.03	33.14	39.35	60.65	0.046 8	0.477 3	1.913 2	0.363 4
	平均值		12.79	52.89	34.32	39.24	60.76
SNP4			GG	GA	AA	G	A
	H	35	2.86	42.86	54.28	24.29	75.71	0.327 9	0.367 8	1.581 7	0.300 1
	EF	17	17.65	52.94	29.41	44.12	55.88	0.761 3	0.493 1	1.972 7	0.371 5
	F₁	193	11.92	60.10	27.98	41.97	58.03	0.001 2	0.487 1	1.949 7	0.368 5
	F₂	169	12.43	57.99	29.58	41.42	58.58	0.011 3	0.485 3	1.942 8	0.367 5
	平均值		11.22	53.47	35.31	37.95	62.05
SNP5			TT	TC	CC	T	C
	H	35	5.71	40.00	54.29	25.71	74.29	0.780 9	0.382 0	1.618 2	0.309 1
	EF	17	23.53	47.06	29.41	47.06	52.94	0.818 8	0.498 3	1.993 1	0.374 1
	F₁	193	12.95	58.03	29.02	41.97	58.03	0.007 9	0.487 1	1.949 7	0.368 5
	F₂	169	13.02	57.40	29.58	41.72	58.28	0.019 1	0.486 3	1.946 6	0.368 0
	平均值		13.80	50.62	35.58	39.11	60.89

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STAT3基因核苷酸序列变异对绵羊泌乳性状的影响

Effect of Nucleotide Sequence Variation of STAT3 on Milk Performance Traits of Sheep

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位点 Locus	基因型 Genotype	数量 Number	乳脂率/% Milk fat percentage	非乳脂固体/% Non-fat milk solid	干物质含量/% Dry matter content	乳蛋白率/% Milk protein percentage	灰分含量/% Ash content	乳糖率/% Milk lactose percentage	日均泌乳量/(kg/d) Average daily milk yield
SNP1	CC	38	6.118±0.530a	11.836±0.211a	17.954±0.552a	5.538±0.175a	0.874±0.017a	5.346±0.020a	0.984±0.090a
	CT	208	5.394±0.278b	11.636±0.110a	17.030±0.289b	5.357±0.092a	0.856±0.009a	5.346±0.010a	0.967±0.047a
	TT	168	4.856±0.125b	11.514±0.265a	16.370±0.327b	5.249±0.104a	0.844±0.010a	5.349±0.012a	0.971±0.053a
	P		0.010	0.164	0.001	0.122	0.103	0.969	0.983
SNP2	CC	53	5.573±0.428a	11.766±0.171a	17.339±0.451a	5.471±0.142a	0.867±0.014a	5.351±0.016a	0.962±0.073a
	CT	206	5.513±0.294ab	11.597±0.117a	17.110±0.309ab	5.324±0.098a	0.852±0.010a	5.342±0.011a	0.975±0.050a
	TT	155	4.745±0.315b	11.553±0.126a	16.298±0.332b	5.282±0.105a	0.848±0.010a	5.354±0.012a	0.964±0.053a
	P		0.014	0.448	0.008	0.399	0.374	0.510	0.965
SNP3	CC	63	5.346±0.426a	11.577±0.166a	16.923±0.451a	5.304±0.138a	0.850±0.014a	5.342±0.015a	0.965±0.069a
	CG	222	5.344±0.292ab	11.608±0.113a	16.953±0.309a	5.332±0.094a	0.853±0.009a	5.346±0.010a	0.995±0.047a
	GG	129	5.083±0.333b	11.628±0.130a	16.711±0.353a	5.351±0.108a	0.855±0.011a	5.352±0.012a	0.920±0.054a
	P		0.019	0.899	0.708	0.884	0.866	0.742	0.253
SNP4	GG	48	5.988±0.444a	11.795±0.175a	17.783±0.467a	5.507±0.145a	0.872±0.014a	5.345±0.016a	0.955±0.074a
	GA	238	5.254±0.285b	11.589±0.112a	16.844±0.299b	5.312±0.093a	0.851±0.009a	5.346±0.010a	0.984±0.048a
	AA	128	4.926±0.335b	11.558±0.132a	16.484±0.352b	5.290±0.110a	0.849±0.011a	5.350±0.012a	0.944±0.056a
	P		0.040	0.520	0.018	0.436	0.381	0.862	0.911
SNP5	TT	53	5.858±0.434a	11.784±0.171a	17.642±0.456a	5.495±0.142a	0.870±0.014a	5.347±0.016a	0.972±0.072a
	TC	231	5.298±0.288ab	11.599±0.113a	16.886±0.303b	5.312±0.094a	0.851±0.009a	5.346±0.011a	0.978±0.048a
	CC	130	4.890±0.332b	11.558±0.130a	16.448±0.349b	5.289±0.108a	0.849±0.011a	5.350±0.012a	0.951±0.055a
	P		0.039	0.538	0.020	0.463	0.410	0.812	1.000

单倍型 Haplotype	碱基序列标签 Base sequence label	单倍型频率/% Haplotype frequency	单倍型组合 Haplotype combination	碱基序列标签 Base sequence label	单倍型组合频率/% Haplotype combination frequency
H1	CGTT	30.77	H1H1	CCGGTTTT	24.02
H2	TCCC	10.88	H1H2	CTGCTCTC	30.64
H3	TCTT	20.37	H1H3	CTGCTTTT	5.15
H4	TCCT	1.90	H1H4	CTGCTCTT	5.88
H5	CCTT	4.87	H1H7	CTGGTCTC	6.37
H6	CCCC	2.60
H7	TGCC	2.18

泌乳性状 Milk performance traits	单倍型组合Haplotype combinations					P值 P value
泌乳性状 Milk performance traits	H1H1(98)	H1H2(125)	H1H3(21)	H1H4(24)	H1H7(26)	P值 P value
乳脂率/% Milk fat percentage	4.745±0.485b	5.456±0.440ab	4.191±0.748b	5.416±0.715ab	6.315±0.640a	0.031
非乳脂固体/% Non-fat milk solid	11.558±0.201a	11.659±0.182a	11.480±0.310a	11.604±0.296a	12.149±0.256a	0.321
干物质含量/% Dry matter content	16.303±0.501b	17.114±0.454ab	15.671±0.772b	17.020±0.739ab	18.464±0.661a	0.022
乳蛋白率/% Milk protein percentage	5.293±0.165a	5.382±0.150a	5.204±0.255a	5.313±0.244a	5.798±0.218a	0.260
灰分含量/% Ash content	0.848±0.016b	0.858±0.015ab	0.840±0.025ab	0.851±0.024ab	0.899±0.022a	0.024
乳糖率/% Milk lactose percentage	5.354±0.019a	5.347±0.017a	5.364±0.029a	5.344±0.028a	5.369±0.025a	0.884
日均泌乳量/kg Average daily milk yield	0.962±0.077ab	1.049±0.070a	1.045±0.119ab	1.011±0.114ab	0.783±0.102b	0.023

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STAT3基因核苷酸序列变异对绵羊泌乳性状的影响

Effect of Nucleotide Sequence Variation of STAT3 on Milk Performance Traits of Sheep

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