表油菜素内酯对遮光处理下马铃薯生理、产量及LHcb基因表达的影响

doi:10.7668/hbnxb.20194796

摘要/Abstract

摘要：

为探究如何缓解马铃薯生产中由于光照不足带来的影响,以马铃薯品种东农310为试验材料,设置 3 个遮光处理:正常光照(Z₀,遮光率为 0)、单层遮光网遮盖处理(Z₁)、双层遮光网遮盖处理(Z₂)。在马铃薯块茎形成期喷施不同浓度表油菜素内酯(0 mg/L,记作CK;0.02 mg/L,记作E₁;0.10 mg/L,记作E₂)。设置3个测定期,对株高、叶绿素a含量、叶绿素b含量、光合参数、荧光动力学参数、单株产量等指标进行测定分析;并测定植株叶片 12 条LHcb基因相对表达量。结果表明,Z₁条件下较为适合东农310生长,而Z₀条件下产生光抑制,各指标较Z₁降低;Z₂条件下光照不足,各指标较Z₁降低。喷施EBR能够有效提高单株产量,在Z₀条件下E₂处理较CK显著提升32.62%,Z₁条件下E₁、E₂处理分别较CK显著提升48.38%,24.24%,Z₂条件下E₂处理较CK显著提升18.45%;在单株块茎干物质质量方面,Z₀条件下E₂处理较CK提升 27.24%,Z₁条件下E₁、E₂处理较CK提升40.09%,26.56%,且差异显著。遮光程度增加和喷施 EBR 可提高12条LHcb基因相对表达量,有利于叶片提高光能的捕获与利用。

关键词: 马铃薯, 遮光, 表油菜素内酯, 光合作用, 荧光参数, 基因表达, 产量

Abstract:

In order to investigate how to alleviate the effects of insufficient light in potato production,the potato variety Dongnong 310 was used as the test material,and three shading treatments were set up:normal light(Z₀,blackout rate is 0),single-layer blackout screen covering treatment(Z₁),double-layer blackout screen covering treatment(Z₂).Different concentrations of epbrassinolide(0 mg/L,denoted as CK)were sprayed in the potato tuber formation stage(0.02 mg/L,denoted as E₁;0.10 mg/L,labeled E₂).Three measuring periods were set,the plant height,chlorophyll a,b content,photosynthetic parameters,fluorescence kinetic parameters,and yield per plant were measured and analyzed.The relative expression levels of LHcb genes in 12 leaves were measured.The results showed that Z₁ conditions were more suitable for the growth of Dongnong 310,while Z₀ conditions produced photoinhibition,and the indexes were lower than Z₁.Under the condition of Z₂,the illumination was insufficient,and the indexes were lower than that of Z₁.EBR spray could effectively increase the yield per plant,and E₂ treatment under Z₀ condition significantly increased by 32.62% compared with CK,E₁ and E₂ treatment under Z₁ condition significantly increased by 48.38% and 24.24% compared with CK,respectively,E₂ treatment under Z₂ condition significantly increased by 18.45% compared with CK.In terms of weight of dry matter per tuber,E₂ treatment under Z₀ condition increased by 27.24% compared with CK,and E₁ and E₂ treatment under Z₁ condition increased by 40.09% and 26.56% compared with CK,and the above differences were significant.The relative expression of 12 LHcb genes could be increased by increasing the shading degree and spraying EBR,which was conducive to improving the light energy capture and utilization in leaves.

Key words: Potato, Shading, 2,4-epibrassinolide, Photosynthesis, Fluorescence parameters, Gene expression, Yield

裴雅婷, 刘玉霖, 李世伟, 张瑀瑶, 高红秀, 唐鑫华, 石瑛. 表油菜素内酯对遮光处理下马铃薯生理、产量及LHcb基因表达的影响[J]. 华北农学报, 2024, 39(4): 133-142. doi: 10.7668/hbnxb.20194796.

PEI Yating, LIU Yulin, LI Shiwei, ZHANG Yuyao, GAO Hongxiu, TANG Xinhua, SHI Ying. Effects of Epbrassinolide on Physiology,Yield and LHcb Gene Expression of Potato under Shading Treatment[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 133-142. doi: 10.7668/hbnxb.20194796.

http://www.hbnxb.net/CN/Y2024/V39/I4/133

图/表 8

参考文献 47

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基因 Gene	上游引物(5'—3') Forward primer(5'—3')	下游引物(5'—3') Reverse primer(5'—3')
Ef1α	GATGTTGTGCCAAAGGATGT	AACTTGGTCAATGCGAGA
Cp24	TTGAAATGGTACAGAGAGG	AGAAGTGTGCCAAAAGAGA
Cp1c	GAGGCTGTGTGGTTCAAG	AACTGCCGCCAGGGTAGA
Cp1b	TTCTTCCCCTTCTTTTGC	TATCCCACCCGTAGTCAC
LHcb1	AAACTCGCACCATCTTCTT	TATCCCATCCATAGTCACC
Cp40	TGACTATGGATGGGACAC	CTCACTGAAAATCTGGGA
Cp6a	GCCCCGTCCATCCTACCT	CCTAAGCCCAATGCCTCT
LHca4	TGTAACGACGCAAGCCTC	CAAAACCATTGTCACCAG
Cp8	CGGTGACTTTGGCTTTGAT	TGCTGTTTCTTGTGGGATG
Cp2	TTAGGTCTTGCTGATGAT	CGTTGTTGTTGACTGGGT
LHcb11	AAACTCTCACCATCTTCTT	TCCCATCCATAGTCACCAG
Cp3c	GTTGAGGGTTACCGTATTGC	TTGATTTCTTTTACTTTGAG
LHcb2	ACCTTATCCATGCTCAGAG	AGAACATAGCCAATCGCCC

基因 Gene	上游引物(5'—3') Forward primer(5'—3')	下游引物(5'—3') Reverse primer(5'—3')
Ef1α	GATGTTGTGCCAAAGGATGT	AACTTGGTCAATGCGAGA
Cp24	TTGAAATGGTACAGAGAGG	AGAAGTGTGCCAAAAGAGA
Cp1c	GAGGCTGTGTGGTTCAAG	AACTGCCGCCAGGGTAGA
Cp1b	TTCTTCCCCTTCTTTTGC	TATCCCACCCGTAGTCAC
LHcb1	AAACTCGCACCATCTTCTT	TATCCCATCCATAGTCACC
Cp40	TGACTATGGATGGGACAC	CTCACTGAAAATCTGGGA
Cp6a	GCCCCGTCCATCCTACCT	CCTAAGCCCAATGCCTCT
LHca4	TGTAACGACGCAAGCCTC	CAAAACCATTGTCACCAG
Cp8	CGGTGACTTTGGCTTTGAT	TGCTGTTTCTTGTGGGATG
Cp2	TTAGGTCTTGCTGATGAT	CGTTGTTGTTGACTGGGT
LHcb11	AAACTCTCACCATCTTCTT	TCCCATCCATAGTCACCAG
Cp3c	GTTGAGGGTTACCGTATTGC	TTGATTTCTTTTACTTTGAG
LHcb2	ACCTTATCCATGCTCAGAG	AGAACATAGCCAATCGCCC

测定时期/(月-日) Determination period	性状指标 Trait indicator	Z₀			Z₁			Z₂
测定时期/(月-日) Determination period	性状指标 Trait indicator	CK	E₁	E₂	CK	E₁	E₂	CK	E₁	E₂
06-18	株高/cm	46.33±0.58a	48.67±0.58a	48.00±1.00a	40.67±1.53a	41.67±0.58a	43.33±0.58a	54.23±1.37a	55.67±2.08a	54.67±1.53a
	SPAD	38.17±0.30a	38.29±0.38a	38.00±0.02a	36.20±0.38a	36.87±0.28a	36.53±0.35a	40.67±0.69a	40.60±0.18a	40.53±0.57a
	叶绿素a/(mg/L)	7.12±0.13a	7.17±0.18a	7.22±0.17a	6.09±0.13a	6.12±0.16a	6.11±0.15a	8.11±0.23a	8.18±0.23a	8.13±0.26a
	叶绿素b/(mg/L)	2.62±0.07a	2.67±0.09a	2.69±0.08a	2.50±0.09a	2.62±0.09a	2.58±0.06a	3.17±0.11a	3.20±0.10a	3.24±0.11a
07-16	株高/cm	69.00±3.28b	77.17±1.61a	72.67±3.40ab	76.00±4.19b	76.17±5.06b	87.00±3.61a	89.17±4.25a	85.00±4.00a	90.83±3.88a
	SPAD	38.77±0.37b	41.90±0.41a	40.03±0.31a	39.60±0.30b	42.33±0.36a	44.00±0.31a	41.83±0.19b	43.67±0.21b	43.93±0.48a
	叶绿素a/(mg/L)	10.21±0.13a	10.44±0.24a	10.63±0.28a	9.86±0.14a	11.91±0.31a	10.09±0.28a	11.49±0.32a	11.86±0.28a	12.46±0.25a
	叶绿素b/(mg/L)	3.78±0.10a	4.09±0.13a	4.15±0.11a	4.35±0.12a	4.65±0.13a	4.53±0.13a	4.64±0.14a	4.80±0.13a	4.89±0.14a
08-13	株高/cm	76.67±3.66b	90.33±4.37a	83.67±5.45ab	101.33±6.71ab	97.67±6.66b	107.67±2.52a	106.00±6.00b	117.67±4.04a	120.33±5.13a
	SPAD	33.93±0.23b	38.60±0.10a	38.30±0.42a	33.70±0.27b	34.17±0.45b	39.43±0.36a	35.70±0.30b	38.80±0.22a	39.57±0.29a
	叶绿素a/(mg/L)	7.21±0.21b	8.29±0.19a	8.43±0.23a	7.05±0.14b	8.73±0.22a	7.23±0.19b	9.39±0.24b	9.97±0.16ab	10.11±0.14a
	叶绿素b/(mg/L)	2.65±0.09b	3.10±0.09ab	3.30±0.08a	3.17±0.10a	3.39±0.10a	3.39±0.08a	3.69±0.11a	3.80±0.09a	3.81±0.10a

测定时期/(月-日) Determination period	性状指标 Trait indicator	Z₀			Z₁			Z₂
测定时期/(月-日) Determination period	性状指标 Trait indicator	CK	E₁	E₂	CK	E₁	E₂	CK	E₁	E₂
06-18	株高/cm	46.33±0.58a	48.67±0.58a	48.00±1.00a	40.67±1.53a	41.67±0.58a	43.33±0.58a	54.23±1.37a	55.67±2.08a	54.67±1.53a
	SPAD	38.17±0.30a	38.29±0.38a	38.00±0.02a	36.20±0.38a	36.87±0.28a	36.53±0.35a	40.67±0.69a	40.60±0.18a	40.53±0.57a
	叶绿素a/(mg/L)	7.12±0.13a	7.17±0.18a	7.22±0.17a	6.09±0.13a	6.12±0.16a	6.11±0.15a	8.11±0.23a	8.18±0.23a	8.13±0.26a
	叶绿素b/(mg/L)	2.62±0.07a	2.67±0.09a	2.69±0.08a	2.50±0.09a	2.62±0.09a	2.58±0.06a	3.17±0.11a	3.20±0.10a	3.24±0.11a
07-16	株高/cm	69.00±3.28b	77.17±1.61a	72.67±3.40ab	76.00±4.19b	76.17±5.06b	87.00±3.61a	89.17±4.25a	85.00±4.00a	90.83±3.88a
	SPAD	38.77±0.37b	41.90±0.41a	40.03±0.31a	39.60±0.30b	42.33±0.36a	44.00±0.31a	41.83±0.19b	43.67±0.21b	43.93±0.48a
	叶绿素a/(mg/L)	10.21±0.13a	10.44±0.24a	10.63±0.28a	9.86±0.14a	11.91±0.31a	10.09±0.28a	11.49±0.32a	11.86±0.28a	12.46±0.25a
	叶绿素b/(mg/L)	3.78±0.10a	4.09±0.13a	4.15±0.11a	4.35±0.12a	4.65±0.13a	4.53±0.13a	4.64±0.14a	4.80±0.13a	4.89±0.14a
08-13	株高/cm	76.67±3.66b	90.33±4.37a	83.67±5.45ab	101.33±6.71ab	97.67±6.66b	107.67±2.52a	106.00±6.00b	117.67±4.04a	120.33±5.13a
	SPAD	33.93±0.23b	38.60±0.10a	38.30±0.42a	33.70±0.27b	34.17±0.45b	39.43±0.36a	35.70±0.30b	38.80±0.22a	39.57±0.29a
	叶绿素a/(mg/L)	7.21±0.21b	8.29±0.19a	8.43±0.23a	7.05±0.14b	8.73±0.22a	7.23±0.19b	9.39±0.24b	9.97±0.16ab	10.11±0.14a
	叶绿素b/(mg/L)	2.65±0.09b	3.10±0.09ab	3.30±0.08a	3.17±0.10a	3.39±0.10a	3.39±0.08a	3.69±0.11a	3.80±0.09a	3.81±0.10a

测定时期/(月-日) Determination period	光合指标 Photosynthetic indicators	Z₀
测定时期/(月-日) Determination period	光合指标 Photosynthetic indicators	CK	E₁	E₂	CK	E₁	E₂	CK	E₁	E₂
06-18	净光合速率/(mmol/(m²·s))	5.90±0.20a	5.70±0.19a	5.85±0.19a	6.89±0.19a	6.78±0.22a	6.85±0.12a	4.33±0.13a	4.41±0.10a	4.43±0.11a
	蒸腾速率/(mmol/(m²·s))	3.36±0.11a	3.53±0.12a	3.44±0.09a	3.33±0.05a	3.44±0.12a	3.41±0.07a	1.91±0.10a	1.97±0.06a	1.95±0.06a
	气孔导度/(μmol/(m²·s))	139.40±5.15a	141.03±4.81a	143.93±4.90a	161.30±7.95a	163.17±6.04a	169.17±7.25a	95.30±5.81a	95.60±5.20a	97.10±4.98a
07-16	净光合速率/(mmol/(m²·s))	7.42±0.25b	8.05±0.17ab	9.76±0.25a	7.95±0.20b	12.87±0.33a	11.59±0.17a	5.43±0.16b	8.10±0.20a	6.74±0.17ab
	蒸腾速率/(mmol/(m²·s))	3.67±0.11b	4.13±0.12ab	4.67±0.14a	4.30±0.11a	4.98±0.10a	4.54±0.16a	2.77±0.09b	3.18±0.11ab	3.61±0.10ab
	气孔导度/(μmol/(m²·s))	166.91±10.09b	207.06±9.78a	225.64±10.22a	179.09±7.57b	227.17±10.13a	193.79±9.42a	154.11±5.66a	162.91±6.03a	196.82±8.39a
08-13	净光合速率/(mmol/(m²·s))	5.01±0.19a	5.40±0.17a	6.88±0.15a	6.72±0.16b	8.49±0.21a	7.44±0.17ab	4.46±0.13a	5.62±0.12a	5.64±0.18a
	蒸腾速率/(mmol/(m²·s))	2.49±0.11b	2.58±0.07b	3.63±0.16a	2.39±0.03b	3.24±0.11a	2.42±0.04b	1.78±0.06b	2.32±0.11ab	2.76±0.10a
	气孔导度/(μmol/(m²·s))	96.52±4.81a	108.91±4.10a	126.48±5.28a	114.32±3.93a	144.69±4.90a	138.72±5.27a	104.04±3.63a	146.18±4.37a	148.37±7.51a

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