感染白绢病对魔芋内源激素含量及相关基因表达的影响

doi:10.7668/hbnxb.20195419

摘要/Abstract

摘要：

为了解魔芋感染白绢病后内源激素与相关基因表达量的变化,进而揭示魔芋参与对白绢病响应的主要激素通路,采用液相色谱串联质谱对3月龄珠芽魔芋感染白绢病0,1,3,6 d 的内源激素含量进行测定,并利用实时荧光定量PCR技术对脱落酸、茉莉酸和水杨酸通路基因表达水平进行分析。结果表明: 感染白绢病后会导致魔芋多种内源激素含量发生变化,随着感染白绢病时间的延长,其中生长素类代谢物吲哚-3-乙酸含量呈先升高后下降的趋势,而吲哚-3-丁酸含量呈下降的趋势;反式玉米素含量则显著下降;脱落酸的含量呈先升高后下降的趋势;茉莉酸类代谢物含量和水杨酸类代谢物的含量均显著高于对照组。魔芋感染白绢病1,3,6 d茉莉酸含量分别为对照组的2.31,2.31,5.08倍,水杨酸含量分别为对照组的5.53,4.60,7.38倍。脱落酸、茉莉酸和水杨酸代谢通路相关的8个基因均被激活,表达量显著提高。感染白绢病后打破了魔芋内源激素稳态,激活了魔芋茉莉酸、水杨酸及脱落酸激素通路相关基因的表达,茉莉酸和水杨酸通路在魔芋对白绢病抗性胁迫中可能具有重要的作用。

关键词: 珠芽魔芋, 白绢病, 植物激素, 基因表达

Abstract:

To understand the changes in endogenous hormones and related gene expression levels after konjac infection with southern blight disease and reveal the main hormone pathways involved in konjac's response to southern blight disease,the ultra performance liquid chromatography (UPLC) and tandem mass spectrometry (MS/MS) was employed to detect the changes in the contents of phytohormone (auxin,abscisic acid,trans-zeatin nucleoside,jasmonic acid,and salicylic acid) in the three-month-old Amorphophallus muelleri,which was infected with southern blight disease for 0,1,3 and 6 days.Moreover,the gene expression levels of abscisic acid,jasmonic acid,and salicylic acid pathways was analyzed by Real-time Quantitative PCR.The results showed that southern blight disease caused changes in the levels of various endogenous hormones in konjac.The content of indole-3-acetic acid tended to increased and then decreased with the southern blight disease infection,while the content of indole-3-butyric acid showed tended to decrease;the content of trans-zeatin nucleoside significant decreased with the infection of southern blight disease;the content of abscisic acid showed a significant increase followed by a decrease with the infection of southern blight disease;the content of jasmonic acid metabolites and salicylic acid metabolites were significantly increased than the control group with the infection of southern blight disease.The content of jasmonic acid of konjac infected with southern blight disease 1,3,and 6 d was 2.31,2.31,and 5.08 times that of the control group,and the content of salicylic acid was 5.53,4.60,and 7.38 times that of the control group,respectively.Eight genes related to abscisic acid,jasmonic acid,and salicylic acid pathways were activated and participated in the response process of konjac to southern blight disease.The above results indicated that the endogenous hormone homeostasis was disrupted after konjac infection with southern blight disease,activated the expression of plant hormone pathway related genes,and jasmonic acid and salicylic acid might play an important role in konjac's resistance to southern blight disease.

Key words: Amorphophallus muelleri, Southern blight disease, Plant hormone, Gene expression

中图分类号:

S436.32

高鹏华, 齐颖, 杨敏, 李丽芳, 黄飞燕, 刘佳妮, 赵建荣, 余磊. 感染白绢病对魔芋内源激素含量及相关基因表达的影响[J]. 华北农学报, 2025, 40(3): 149-156. doi: 10.7668/hbnxb.20195419.

GAO Penghua, QI Ying, YANG Min, LI Lifang, HUANG Feiyan, LIU Jiani, ZHAO Jianrong, YU Lei. Effect of Southern Blight Disease on Endogenous Hormone Content and Related Gene Expression Levels in Konjac[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 149-156. doi: 10.7668/hbnxb.20195419.

http://www.hbnxb.net/CN/Y2025/V40/I3/149

图/表 7

表1 荧光定量PCR引物序列

Tab.1 Real-time Fluorescence Quantitative PCR primer sequences

引物名称 Primer name	引物序列(5'—3') Primer sequence (5'—3')	产物大小/bp Product length
PP2C-F	ATGCCTGATCCTTGCGAGTG	140
PP2C-R	CAGGATCAGCTCCACCTCTC
ABF-F	AGAGCAAGCAGAAGGGAACC	107
ABF-R	CGGCTGCTTTACCTCCATCA
AOC-F	ATGGGAACCTGAAGAAGCGG	100
AOC-R	CTGTAGATGGCCTCGTAGCG
OPR4-F	GACAAGGAGGAGGGCAACAG	112
OPR4-R	GCGCGTTGATCTGAAACCTC
ACX-F	AGATTTGCCGCTTTCTTGGC	190
ACX-R	GTAGAAGCCGCCGTTGATGA
NPR-F	ATGAGAACAGTGGAGCTGGG	196
NPR-R	CGCTCTCCGCCTTATCCTTG
JAZ-F	GAGACGGGACTTGACGGAAC	118
JAZ-R	CCAAGAACCTCTGGAGCGAC
TGA1-F	CGAGTTCTTCCGCCAGGTC	162
TGA1-R	GGCGAGAACCCATTGTAGGC
Actin-F	GGTCCTGTTCCAGCCATCTC	142
Actin-R	GAACATGGTAGACCCTCCGC

图1 生长素类代谢物含量变化

Fig.1 Change of auxin metabolites content

图2 反式玉米素含量变化

Fig.2 Change of trans-zeatin riboside content

图3 脱落酸含量变化

Fig.3 Change of abscisic acid content

图4 茉莉酸类代谢物含量变化

Fig.4 Change of jasmonic acid metabolites content

图5 水杨酸类代谢物含量变化

Fig.5 Change of salicylic acid metabolites content

图6 ABA (A)、JA (B)和SA (C)通路基因表达分析不同小写字母表示处理间有显著差异(P<0.05)。

Fig.6 Analysis of gene expression levels in ABA (A),JA (B) and SA(C) pathway There were significant differences between different lowercase letters (P<0.05).

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