假禾谷镰孢GATA转录因子的鉴定及<i>FpGATA5</i>的功能分析

doi:10.7668/hbnxb.20194993

摘要/Abstract

摘要：

GATA转录因子在调控植物病原真菌菌丝生长、产孢、孢子萌发和致病性等过程中具有重要作用。为探究GATA转录因子在假禾谷镰孢致病过程中的功能,基于同源检索和蛋白质结构域鉴定方法,在假禾谷镰孢基因组中鉴定到7个GATA转录因子编码基因,分别命名为FpGATA1~FpGATA7。FpGATA基因长度为614~3 155 bp,含有1~3个内含子。FpGATA蛋白均含有保守的GATA结构域,等电点显示FpGATA多为碱性。采用实时荧光定量PCR分析FpGATA在假禾谷镰孢不同侵染阶段的相对表达量,发现FpGATA1、FpGATA4、FpGATA5、FpGATA6和FpGATA7在侵染早期诱导表达,FpGATA2在侵染后期表达量升高。利用真菌遗传转化方法获得假禾谷镰孢FpGATA5缺失的突变体,表型分析发现,与野生型相比,FpGATA5缺失突变体的菌丝生长速率和分生孢子产量均明显下降,接种大麦叶片和小麦胚芽鞘的致病力显著降低。综合上述结果,多个FpGATA基因的表达响应假禾谷镰孢的致病过程,且FpGATA5参与病原菌的生长、产孢和致病。

关键词: 小麦茎基腐病, 假禾谷镰孢, GATA转录因子, 基因表达, 致病力

Abstract:

GATA transcription factors play important roles in regulating hyphal growth,conidial production,conidial germination and virulence in phytopathogenic fungi.In order to explore the function of GATA transcription factors in Fusarium pseudograminearum,a total of seven GATA transcription factors encoding genes,which were subsequently named as FpGATA1-FpGATA7,were identified from the genome of F.pseudograminearum using homology searching and domain identification.The full-length sequences of FpGATA genes were 614-3 155 bp,containing one,two or three introns.All FpGATA proteins contained at least one conserved GATA domain,and most of these proteins were alkaline.The quantitative Real-time PCR was used to assay the relative expression levels of FpGATA genes during different infection stages.We found that FpGATA1,FpGATA4,FpGATA5,FpGATA6 and FpGATA7 were induced during the early infection stages,and FpGATA2 was induced in the later infection stage.The FpGATA5 was deleted in F.pseudograminearum by genetic transformation.Compared to the wild-type,the FpGATA5 deletion mutants showed a decrease in hyphal growth and conidial production,and the significantly reduced pathogenicity was observed by barely leaves and wheat coleoptiles inoculation.Overall,the results suggested that the expression of several FpGATA genes was induced during F.pseudograminearum infection,and FpGATA5 played roles in growth,conidiation and pathogenesis of F.pseudograminearum.

Key words: Fusarium crown rot, Fusarium pseudograminearum, GATA transcription factor, Gene expression, Pathogenicity

张时雨, 赵培怡, 刘敏, 朱文亭, 彭梦雅, 施艳, 杨雪, 李洪连, 陈琳琳. 假禾谷镰孢GATA转录因子的鉴定及FpGATA5的功能分析[J]. 华北农学报, 2024, 39(5): 204-211. doi: 10.7668/hbnxb.20194993.

ZHANG Shiyu, ZHAO Peiyi, LIU Min, ZHU Wenting, PENG Mengya, SHI Yan, YANG Xue, LI Honglian, CHEN Linlin. Identification of GATA Transcription Factors in Fusarium pseudograminearum and Functional Analysis of FpGATA5[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 204-211. doi: 10.7668/hbnxb.20194993.

http://www.hbnxb.net/CN/Y2024/V39/I5/204

图/表 9

表1 本研究中所用的引物

Tab.1 Primers used in this study

引物名称 Primer name	引物序列 (5'-3') Primer sequence (5'-3')	用途 Purpose
FpGATA1-RTF	TCGGACATGACGCCTTCTGAAG	实时荧光
FpGATA1-RTR	TCGAGGTTCTCGCGCGTGAGTG	定量PCR
FpGATA2-RTF	ATGTTCAACTTCACTTCGAATC
FpGATA2-RTR	ATTGCTCACTCAAGCACGAAC
FpGATA3-RTF	TGCGATCCACACCTTATGAGTAC
FpGATA3-RTR	ACAGGTAGCAGCGCTTCGACTTG
FpGATA4-RTF	TCGCACCAGGTACTCAGGATG
FpGATA4-RTR	TCCGAGTTCCTCGGCCAATATG
FpGATA5-RTF	AGTCGACCTGCAAGCAGCAAGG
FpGATA5-RTR	ACCCATAGCACCAGATGAGGCAC
FpGATA6-RTF	TCGATACGACCCAACAGAAC
FpGATA6-RTR	TGGCTGCCATCGATGCCATG
FpGATA7-RTF	CACCCAATCGGCTCAGACCCTG
FpGATA7-RTR	TCTAGATGCTTCAGAAGCGATG
TEF1a-RTF	TCACCACTGAAGTCAAGTCC
TEF1a-RTR	ACCAGCGACGTTACCACGTC
FpGATA5-F1	CGGGCCTAAGTGTTGTGG	FpGATA5
FpGATA5-R1	TTGACCTCCACTAGCTCCAGCCAA GCCGGTTAGAACAGGACTTGAG	基因敲除盒的构建
FpGATA5-F2	GAATAGAGTAGATGCCGACCGCG GGTTCGTAAGGGGACTTGTGTGC
FpGATA5-R2	TAGCGGGCATAAGGTTAG
HYG-F	GGCTTGGCTGGAGCTAGTGGAGGTCAA
HYG-R	GAACCCGCGGTCGGCATCTACTCTAT
YG-F	GATGTAGGAGGGCGTGGATATGTCCT
HY-R	GTATTGACCGATTCCTTGCGGTCCGAA
FpGATA5-GF3	ATTCTCGTGACGTCTCGG	FpGATA5
FpGATA5-GR3	GTGGCGGTTGACATAGTT	基因缺失
H852F	TTCCTCCCTTTATTTCAGATTCAA	突变体的
H850R	ATGTTGGCGACCTCGTATTGG	检测
FpGATA5-F4	TCTTGTCTTGTTTACCCATC
H855R	GCTGATCTGACCAGTTGC
H856F	GTCGATGCGACGCAATCGT
FpGATA5-R4	CATCGCACTCCAATGAAC

表2 FpGATA基因信息

Tab.2 Information on FpGATA genes

基因名称 Genename	转录号 Transcript ID	基因长度/bp Gene length	内含子数量/个 Number of intron	氨基酸数量/个 Amino acid number	分子质量/ku Molecular weight	等电点 pI
FpGATA1	FPSE_00322	1 627	2	483	44.89	5.55
FpGATA2	FPSE_08455	634	1	193	17.93	10.26
FpGATA3	FPSE_09876	614	1	184	17.08	9.55
FpGATA4	FPSE_10791	3 155	1	1 033	95.72	8.21
FpGATA5	FPSE_10693	3 092	1	949	87.70	9.20
FpGATA6	FPSE_00936	1 778	2	557	51.56	9.41
FpGATA7	FPSE_04965	2 013	3	470	43.55	4.53

图1 FpGATA基因的外显子-内含子结构

Fig.1 Exon-intron structure of FpGATA genes

图2 FpGATA蛋白结构域分布

Fig.2 Distrubution of FpGATA protein domains

图3 FpGATA基因在假禾谷镰孢不同侵染阶段的相对表达水平不同小写字母表示与MY阶段差异显著(P<0.05)。

Fig.3 Relative expression levels of FpGATA genes during different infection stages of Fusarium pseudograminearum Different lowercase letters indicate significant(P<0.05)difference compared to MY stage,respectively.

图4 FpGATA5缺失突变体的PCR检测 M.DL2000 DNA Marker; G.FpGATA5基因;F.FpGATA5上游与潮霉素抗性基因融合片段; H.HPH基因; R.潮霉素抗性基因与FpGATA5下游融合片段。WT.野生型菌株。

Fig.4 PCR products of FpGATA5 deleted-mutants M.DL2000 DNA Marker; G.FpGATA5 gene;F.The FpGATA5 upstream and HPH cassette;H.HPH gene;R.The HPH and FpGATA5 down stream cassette.WT.The wild type strain.

图5 FpGATA5缺失突变体的菌丝生长情况 A.野生型和FpGATA5缺失突变体的菌落;B.野生型和FpGATA5缺失突变体的菌落直径。WT.野生型菌株。不同大写字母表示在0.01水平上差异显著。图6,7同。

Fig.5 Hyphal growth of FpGATA5 deleted-mutants A.Colonies of the wild type and FpGATA5 deleted-mutants;B.Colony diameters of the wild type and FpGATA5 deleted-mutants. WT.The wild type strain.Different capital letters indicate extremely significant difference at the 0.01 level.The same as Fig.6,7.

图6 FpGATA5缺失突变体的分生孢子产生情况 A.野生型和FpGATA5缺失突变体的孢子梗和分生孢子;B.野生型和FpGATA5缺失突变体的分生孢子量。

Fig.6 Conidial production of FpGATA5 deleted-mutants A.Conidiophores and conidia of the wild type and FpGATA5 deleted-mutants;B.Conidial number of the wild type and FpGATA5 deleted-mutants.

图7 FpGATA5缺失突变体的致病力情况 A.野生型和FpGATA5缺失突变体接种的大麦叶片和小麦胚芽鞘;B.大麦叶片和小麦胚芽鞘上坏死病斑的长度。

Fig.7 Virulence of FpGATA5 deleted-mutants A.The barley leaves and wheat coleoptiles inoculated by the wild type and FpGATA5 deleted-mutants;B.Lesion length on barley leaves and wheat coleoptiles.

参考文献 36

[1]	Singh R P, Singh P K, Rutkoski J, Hodson D P, He X Y, Jørgensen L N, Hovmøller M S, Huerta-Espino J. Disease impact on wheat yield potential and prospects of genetic control[J]. Annual Review of Phytopathology, 2016, 54:303-322.doi:10.1146/annurev-phyto-080615-095835. pmid: 27296137
[2]	Kazan K, Gardiner D M. Fusarium crown rot caused by Fusarium pseudograminearums in cereal crops:recent progress and future prospects[J]. Molecular Plant Pathology, 2018, 19(7):1547-1562.doi:10.1111/mpp.12639.
[3]	Powell J J, Carere J, Fitzgerald T L, Stiller J, Covarelli L, Xu Q, Gubler F, Colgrave M L, Gardiner D M, Manners J M, Henry R J, Kazan K. The Fusarium crown rot pathogen Fusarium pseudograminearum triggers a suite of transcriptional and metabolic changes in bread wheat (Triticum aestivum L.)[J]. Annals of Botany, 2017, 119(5):853-867.doi:10.1093/aob/mcw207.
[4]	Hogg A C, Johnston R H, Johnston J A, Klouser L, Kephart K D, Dyer A T. Monitoring Fusarium crown rot populations in spring wheat residues using quantitative real-time polymerase chain reaction[J]. Phytopathology, 2010, 100(1):49-57.doi:10.1094/PHYTO-100-1-0049. pmid: 19968549
[5]	Zhou H F, He X L, Wang S, Ma Q Z, Sun B J, Ding S L, Chen L L, Zhang M, Li H L. Diversity of the Fusarium pathogens associated with crown rot in the Huanghuai wheat-growing region of China[J]. Environmental Microbiology, 2019, 21(8):2740-2754.doi:10.1111/1462-2920.14602.
[6]	Deng Y Y, Li W, Zhang P, Sun H Y, Zhang X X, Zhang A X, Chen H G. Fusarium pseudograminearum as an emerging pathogen of crown rot of wheat in Eastern China[J]. Plant Pathology, 2020, 69(2):240-248.doi:10.1111/ppa.13122.
[7]	Li H L, Yuan H X, Fu B, Xing X P, Sun B J, Tang W H. First report of causing crown rot of wheat in Henan,China[J]. Plant Disease, 2012, 96:1065.doi:10.1094/PDIS-01-12-0007-PDN. pmid: 30727237
[8]	吴斌, 郭霞, 张眉, 姜珊珊, 辛志梅, 王升吉, 辛相启. 鲁西南地区小麦茎基腐病病原菌鉴定及其致病力分析[J]. 麦类作物学报, 2018, 38(3):358-365.doi:10.7606/j.issn.1009-1041.2018.03.14.
	Wu B, Guo X, Zhang M, Jiang S S, Xin Z M, Wang S J, Xin X Q. Identification and pathogenicity of pathogens associated with the wheat crown rot in the Southwest of Shandong Province[J]. Journal of Triticeae Crops, 2018, 38(3):358-365.
[9]	周海峰, 杨云, 牛亚娟, 袁虹霞, 李洪连. 小麦茎基腐病的发生动态与防治技术[J]. 河南农业科学, 2014, 43(5):114-117.doi:10.3969/j.issn.1004-3268.2014.05.026.
	Zhou H F, Yang Y, Niu Y J, Yuan H X, Li H L. Occurrence and control methods of crown rot of wheat[J]. Journal of Henan Agricultural Sciences, 2014, 43(5):114-117.
[10]	俞慧友. 30个!中国科协发布2022年科技领域重大问题难题[N]. 科技日报, 2022-06-28.doi:10.28502/n.cnki.nkjrb.2022.003291.
	Yu H Y. China association for science and technology released 30 major problems in the field of science and technology in 2022[N]. Science and Technology Daily, 2022-06-28.
[11]	高飞, 谢源, 潘鑫, 李洪连, 张晓婷, 吴祖建, 关雄. 2019-2020年河南省小麦茎基腐病病原菌鉴定及致病力测定[J]. 植物保护学报, 2023, 50(2):298-305.doi:10.13802/j.cnki.zwbhxb.2023.2022050.
	Gao F, Xie Y, Pan X, Li H L, Zhang X T, Wu Z J, Guan X. Identification and virulence determination of pathogens of wheat crown rot in Henan Province in 2019-2020[J]. Journal of Plant Protection, 2023, 50(2):298-305.
[12]	范学锋. 中国小麦茎基腐病病原菌群体组成及遗传结构分析[D]. 北京: 中国农业科学院, 2021.
	Fan X F. Population composition and genetic structure analysis of pathogen of wheat stem rot in China[D]. Beijing: Chinese Academy of Agricultural Sciences, 2021.
[13]	Aoki T, O'Donnell K. Morphological and molecular characterization of Fusarium pseudograminearum sp.nov.,formerly recognized as the group 1 population of F.graminearum[J]. Mycologia, 1999, 91(4):597.doi:10.2307/3761245.
[14]	Fan X F, Yan Z, Yang M X, Waalwijk C, van der Lee T, van Diepeningen A, Brankovics B, Chen W Q, Feng J, Zhang H. Contamination and translocation of deoxynivalenol and its derivatives associated with Fusarium crown rot of wheat in Northern China[J]. Plant Disease, 2021, 105(11):3397-3406.doi:10.1094/PDIS-03-21-0612-RE.
[15]	Obanor F, Neate S, Simpfendorfer S, Sabburg R, Wilson P, Chakraborty S. Fusarium graminearum and Fusarium pseudograminearum caused the 2010 head blight epidemics in Australia[J]. Plant Pathology, 2013, 62(1):79-91.doi:10.1111/j.1365-3059.2012.02615.x.
[16]	Tunali B, Obanor F, Erginbaş G, Westecott R A, Nicol J, Chakraborty S. Fitness of three Fusarium pathogens of wheat[J]. FEMS Microbiology Ecology, 2012, 81(3):596-609.doi:10.1111/j.1574-6941.2012.01388.x. pmid: 22500915
[17]	Bragard C, Baptista P, Chatzivassiliou E, Di Serio F, Gonthier P, Miret J A J, Justesen A F, MacLeod A, Magnusson C S, Milonas P, Navas-Cortes J A, Parnell S, Potting R, Stefani E, Thulke H H, Werf W V D, Civera A V, Yuen J, Zappalà L, Migheli O, Vloutoglou I, Czwienczek E, Maiorano A, Streissl F, Reignault P L. Pest categorization of Fusarium pseudograminearum[J]. European Food Safety Authority, 2022, 20(6):e07399.doi:10.2903/j.efsa.2022.7399.eCollection 2022 Jun.
[18]	Lowry J A, Atchley W R. Molecular evolution of the GATA family of transcription factors:conservation within the DNA-binding domain[J]. Journal of Molecular Evolution, 2000, 50(2):103-115.doi:10.1007/s002399910012. pmid: 10684344
[19]	Reyes J C, Muro-Pastor M I, Florencio F J. The GATA family of transcription factors in Arabidopsis and rice[J]. Plant Physiology, 2004, 134(4):1718-1732.doi:10.1104/pp.103.037788. pmid: 15084732
[20]	Li J T, Mu W H, Veluchamy S, Liu Y Z, Zhang Y H, Pan H Y, Rollins J A. The GATA-type IVb zinc-finger transcription factor SsNsd1 regulates asexual-sexual development and appressoria formation in Sclerotinia sclerotiorum[J]. Molecular Plant Pathology, 2018, 19(7):1679-1689.doi:10.1111/mpp.12651.
[21]	Marroquin-Guzman M, Wilson R A. GATA-dependent glutaminolysis drives appressorium formation in Magnaporthe oryzae by suppressing TOR inhibition of cAMP/PKA signaling[J]. PLoS Pathogens, 2015, 11(4):e1004851.doi:10.1371/journal.ppat.1004851.
[22]	Son H, Seo Y S, Min K, Park A R, Lee J, Jin J M, Lin Y, Cao P J, Hong S Y, Kim E K, Lee S H, Cho A, Lee S, Kim M G, Kim Y, Kim J E, Kim J C, Choi G J, Yun S H, Lim J Y, Kim M, Lee Y H, Choi Y D, Lee Y W. A phenome-based functional analysis of transcription factors in the cereal head blight fungus,Fusarium graminearum[J]. PLoS Pathogens, 2011, 7(10):e1002310.doi:10.1371/journal.ppat.1002310.
[23]	Chen Y N, Cao Y Z, Gai Y P, Ma H J, Zhu Z R, Chung K R, Li H Y. Genome-wide identification and functional characterization of GATA transcription factor gene family in Alternaria alternata[J]. Journal of Fungi, 2021, 7(12):1013.doi:10.3390/jof7121013.
[24]	Liu L, Wang Q C, Sun Y, Zhang Y H, Zhang X H, Liu J L, Yu G, Pan H Y. Sssfh1,a gene encoding a putative component of the RSC chromatin remodeling complex,is involved in hyphal growth,reactive oxygen species accumulation,and pathogenicity in Sclerotinia sclerotiorum[J]. Frontiers in Microbiology, 2018, 9:1828.doi:10.3389/fmicb.2018.01828.
[25]	赵静雅, 夏荟清, 彭梦雅, 凡卓, 殷悦, 徐赛博, 张楠, 陈文波, 陈琳琳. 假禾谷镰孢转录因子FpAPSES的鉴定与功能分析[J]. 中国农业科学, 2021, 54(16):3428-3439.doi:10.3864/j.issn.0578-1752.2021.16.006.
	Zhao J Y, Xia H Q, Peng M Y, Fan Z, Yin Y, Xu S B, Zhang N, Chen W B, Chen L L. Identification and functional analysis of transcription factors FpAPSES in Fusarium pseudograminearum[J]. Scientia Agricultura Sinica, 2021, 54(16):3428-3439.
[26]	Wang L M, Zhang Y F, Du Z L, Kang R J, Chen L L, Xing X P, Yuan H X, Ding S L, Li H L. FpPDE1 function of Fsarium pseudograminearum on pathogenesis in wheat[J]. Journal of Integrative Agriculture, 2017, 16(11):2504-2512.doi:10.1016/s2095-3119(17)61689-7.
[27]	Merika M, Orkin S H. DNA-binding specificity of GATA family transcription factors[J]. Molecular and Cellular Biology, 1993, 13(7):3999-4010.doi:10.1128/mcb.13.7.3999-4010.1993. pmid: 8321207
[28]	Cooper T G. Transmitting the signal of excess nitrogen in Saccharomyces cerevisiae from the Tor proteins to the GATA factors:connecting the dots[J]. FEMS Microbiology Reviews, 2002, 26(3):223-238.doi:10.1111/j.1574-6976.2002.tb00612.x.
[29]	Cunningham T S, Rai R, Cooper T G. The level of DAL80 expression down-regulates GATA factor-mediated transcription in Saccharomyces cerevisiae[J]. Journal of Bacteriology, 2000, 182(23):6584-6591.doi:10.1128/JB.182.23.6584-6591.2000. pmid: 11073899
[30]	Pelletier B, Beaudoin J, Philpott C C, Labbé S. Fep1 represses expression of the fission yeast Schizosaccharomyces pombe siderophore-iron transport system[J]. Nucleic Acids Research, 2003, 31(15):4332-4344.doi:10.1093/nar/gkg647. pmid: 12888492
[31]	王建霞, 王擎, 赵玉兰, 李天聪, 龙凤, 朱行, 申珅, 郝志敏. 玉米大斑病菌GATA转录因子氮源响应表达模式分析[J]. 农业生物技术学报, 2020, 28(7):1297-1305.doi:10.3969/j.issn.1674-7968.2020.07.016.
	Wang J X, Wang Q, Zhao Y L, Li T C, Long F, Zhu H, Shen S, Hao Z M. Expression patterns analysis of GATA transcription factors in nitrogen source response in Setosphaeria turcica[J]. Journal of Agricultural Biotechnology, 2020, 28(7):1297-1305.
[32]	Liu L, Wang Q C, Zhang X H, Liu J L, Zhang Y H, Pan H Y. Ssams2,a gene encoding GATA transcription factor,is required for appressoria formation and chromosome segregation in Sclerotinia sclerotiorum[J]. Frontiers in Microbiology, 2018, 9:3031.doi:10.3389/fmicb.2018.03031.
[33]	Niehaus E M, Schumacher J, Burkhardt I, Rabe P, Spitzer E, Münsterkötter M, Güldener U, Sieber C M K, Dickschat J S, Tudzynski B. The GATA-type transcription factor Csm1 regulates conidiation and secondary metabolism in Fusarium fujikuroi[J]. Frontiers in Microbiology, 2017, 8:1175.doi:10.3389/fmicb.2017.01175.
[34]	Palos-Fernández R, Turrà D, Di Pietro A. The Gal4-type transcription factor Pro1 integrates inputs from two different MAPK cascades to regulate development in the fungal pathogen Fusarium oxysporum[J]. Journal of Fungi, 2022, 8(12):1242.doi:10.3390/jof8121242.
[35]	Chen L L, Zhao J Y, Xia H Q, Ma Y M, Liu Y K, Peng M Y, Xing X P, Sun B J, Shi Y, Li H L. FpCzf14 is a putative C₂H₂ transcription factor regulating conidiation in Fusarium pseudograminearum[J]. Phytopathology Research, 2020, 2:33.doi:10.1186/s42483-020-00074-7.
[36]	赵静雅, 彭梦雅, 张时雨, 单艺轩, 邢小萍, 施艳, 李海洋, 杨雪, 李洪连, 陈琳琳. C₂H₂锌指转录因子FpCzf7参与假禾谷镰孢的生长和致病性[J]. 生物技术通报, 2022, 38(8):216-224.doi:10.13560/j.cnki.biotech.bull.1985.2021-1457.
	Zhao J Y, Peng M Y, Zhang S Y, Shan Y X, Xing X P, Shi Y, Li H Y, Yang X, Li H L, Chen L L. Role of C₂H₂ zinc finger transcription factor FpCzf7 in the growth and pathogenicity of Fusarium pseudograminearum[J]. Biotechnology Bulletin, 2022, 38(8):216-224.

选择文件类型/文献管理软件名称

选择包含的内容

假禾谷镰孢GATA转录因子的鉴定及FpGATA5的功能分析

Identification of GATA Transcription Factors in Fusarium pseudograminearum and Functional Analysis of FpGATA5

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 36

相关文章 15

编辑推荐

Metrics

本文评价

[1]	高鹏华, 齐颖, 杨敏, 李丽芳, 黄飞燕, 刘佳妮, 赵建荣, 余磊. 感染白绢病对魔芋内源激素含量及相关基因表达的影响[J]. 华北农学报, 2025, 40(3): 149-156.
[2]	王昭懿, 崔原瑗, 韩梦荞, 刘正文, 邓习, 豆飞飞, 任毓昭, 刘彩霞, 刘凤楼, 王掌军, 孙洋洋, 任民, 李清峰. 黑麦NHX基因家族的鉴定及盐胁迫下的表达分析[J]. 华北农学报, 2025, 40(3): 9-18.
[3]	胡雨莹, 孙茂, 汪骞, 黎志彬, 鲍锐, 桂敏, 钟秋月, 杜光辉, 吴丽艳. *野生蒜芥茄SsRPM1基因的生信分析、亚细胞定位及表达分析*[J]. 华北农学报, 2025, 40(3): 27-33.
[4]	蓝湘一, 何洋洋, 张明, 黄慈, 王海波, 王会, 柴志欣, 钟金城. *牦牛MAP3K7基因克隆及其与皮下前体脂肪细胞分化关系分析*[J]. 华北农学报, 2025, 40(3): 225-233.
[5]	马峻, 连凯琪, 闪金研, 刘玉玲, 李晓龙, 赵黎明, 李学军, 彭仁海. 淇河鲫bmp6基因的克隆及表达特性分析[J]. 华北农学报, 2025, 40(2): 218-229.
[6]	徐新瑞, 宋泽龙, 李二峰. 尖孢镰刀菌黏团专化型转录因子SNT2基因敲除及其功能分析[J]. 华北农学报, 2025, 40(2): 173-180.
[7]	牛瑞来, 张悦, 韦应实, 杨飏, 卿宇, 成述儒, 朱才业. 绵羊尾脂沉积相关circRNA的鉴定及功能验证[J]. 华北农学报, 2025, 40(2): 191-200.
[8]	付盼盼, 胡慧慧, 李杰, 赵进, 王鹏飞, 尚学峰, 张森, 黄晓宇. 八眉猪和大白猪乳成分、血清生化及泌乳相关基因的比较分析[J]. 华北农学报, 2025, 40(1): 215-223.
[9]	巩永杰, 田海燕, 魏家萍, 崔俊美, 武泽峰, 董小云, 郑国强, 王莹, 王小霞, 刘自刚. 冬播对冬性/春性甘蓝型油菜萌发与花期生理生化的影响[J]. 华北农学报, 2024, 39(6): 106-114.
[10]	冯韬, 陈勤勤, 杨佳, 谭晖, 尹明智, 胡燕. 外源蔗糖对甘蓝型油菜种子萌发与幼苗发育的影响[J]. 华北农学报, 2024, 39(5): 110-116.
[11]	尹明达, 罗蕊, 任文静, 王志妍, 苏志敏, 李茹鑫, 陈圳, 李玉玲, 王艳, 黄凤兰. *蓖麻PIP5K2基因克隆及功能预测*[J]. 华北农学报, 2024, 39(4): 72-79.
[12]	夏轲, 罗堰木, 黄敏, 杜何为. 玉米DUF668基因家族全基因组鉴定及其表达分析[J]. 华北农学报, 2024, 39(4): 20-30.
[13]	裴雅婷, 刘玉霖, 李世伟, 张瑀瑶, 高红秀, 唐鑫华, 石瑛. 表油菜素内酯对遮光处理下马铃薯生理、产量及LHcb基因表达的影响[J]. 华北农学报, 2024, 39(4): 133-142.
[14]	陶功臣, 马玉杰, 文军琴, 王亚艺, 李全辉. *不同成熟果色辣椒中PSY1基因的差异表达分析*[J]. 华北农学报, 2024, 39(3): 15-24.
[15]	冯伊彤, 贾硕, 刘麟, 杨剑锋, 杜磊, 张文兵, 石胜华, 武占敏, 张键, 赵君. 外源添加铁离子对向日葵大丽轮枝菌生物学特性及致病力的影响[J]. 华北农学报, 2024, 39(2): 161-167.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

假禾谷镰孢GATA转录因子的鉴定及FpGATA5的功能分析

Identification of GATA Transcription Factors in Fusarium pseudograminearum and Functional Analysis of FpGATA5

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 36

相关文章 15

编辑推荐

Metrics

本文评价