粒重基因<em>TaCwi-A1</em>等位变异在黄淮麦区小麦品种(系)中的分布及功能分析

doi:10.7668/hbnxb.2017.02.020

华北农学报 ›› 2017, Vol. 32 ›› Issue (2): 131-137. doi: 10.7668/hbnxb.2017.02.020

所属专题： 小麦；生物技术；

粒重基因TaCwi-A1等位变异在黄淮麦区小麦品种(系)中的分布及功能分析

刘永伟¹, 周硕¹, 王雪征², 孙果忠¹, 朱金永¹, 韩秋芬¹, 李春杰³, 赵和¹, 王海波¹

1. 河北省农林科学院遗传生理研究所, 河北省植物转基因中心, 河北石家庄 050051;
2. 河北省农林科学院旱作农业研究所, 河北衡水 053000;
3. 河北省种子管理总站, 河北石家庄 050031

收稿日期:2017-01-23 出版日期:2017-04-28
通讯作者: 赵和(1962-),男,河北康保人,研究员,硕士,主要从事小麦分子育种研究;王海波(1958-),男,河北满城人,研究员,博士,博士生导师,主要从事作物遗传与生物技术研究。
作者简介:刘永伟(1981-),男,内蒙古乌拉特中旗人,助理研究员,硕士,主要从事作物分子育种研究。
基金资助:
河北省农林科学院科学技术研究与发展计划项目（A2015110102）；河北省现代农业创新工程项目（2017038997）；北京全式金生物技术有限公司Trans助研梦想基金（Trans-RasDF-003）

Functional Analysis and Distribution of Allelic Variations of TaCwi-A1 Gene Related to Kernel Weight in Yellow and Huai River Valleys Facultative Wheat Zone

LIU Yongwei¹, ZHOU Shuo¹, WANG Xuezheng², SUN Guozhong¹, ZHU Jinyong¹, HAN Qiufen¹, LI Chunjie³, ZHAO He¹, WANG Haibo¹

1. Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China;
2. Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Science, Hengshui 053000, China;
3. The Seed Management Station of Hebei Province, Shijiazhuang 050031, China

Received:2017-01-23 Published:2017-04-28

摘要/Abstract

摘要： 为探讨小麦粒重基因TaCwi-A1等位变异TaCwi-A1a和TaCwi-A1b在育种实践中的应用价值，首先利用TaCwi-A1功能标记对539份黄淮麦区小麦品种（系）进行分子检测，确定各供试材料的等位变异类型，从而获得TaCwi-A1a和TaCwi-A1b 2种等位变异的分布频率。对审定品种、参加区试品系以及自育品系进行了千粒质量（3个不同生长环境）及粒长、粒宽和籽粒面积（1个生长环境）测试分析，比较TaCwi-A1a和TaCwi-A1b等位变异籽粒表型性状的差异性。结果表明，在539份黄淮麦区小麦资源中TaCwi-A1a的分布频率为65.03%，TaCwi-A1b的分布频率为34.97%，TaCwi-A1a的分布频率明显高于TaCwi-A1b。籽粒表型性状分析表明，无论是审定品种，还是参加区试品系和自育品系，在3个生长环境下，TaCwi-A1a基因型材料的千粒质量均值都显著高于TaCwi-A1b；TaCwi-A1a基因型材料的粒长和粒宽均显著高于TaCwi-A1b。进一步验证了TaCwi-A1a等位变异的籽粒表型性状增效功能，说明其对粒重及其构成要素是优异的等位变异。此外，本研究鉴定了黄淮麦区中TaCwi-A1等位变异的分布情况，为亲本选配提供了参考。

关键词: 普通小麦, TaCwi-A1, 分子标记, 等位变异, 千粒质量, 粒长, 粒宽

Abstract: In order to evaluate the breeding application value of two kind of allelic variations (TaCwi-A1a and TaCwi-A1b)of TaCwi-A1 gene,which was related to wheat kernel weight.In this study,539 wheat varieties (lines)in Yellow and Huai River Valleys Facultative Wheat Zone in China were genotyped using functional markers of TaCwi-A1 to determine the allelic variation types,in which the frequencies of TaCwi-A1a and TaCwi-A1b could be detected.To compare the differences of phenotypic traits of TaCwi-A1a and TaCwi-A1b,thousand kernel weight (TKW)of released varieties,region test varieties,and breeding varieties was evaluated in three environments;and kernel length (KL),kernel width (KW),and kernel area (KA)was also evaluated in one environment.The results showed that the frequencies of TaCwi-A1a and TaCwi-A1b were 65.03% and 34.97% in all test materials,in which the frequency of TaCwi-A1a was significantly higher than TaCwi-A1b.Analysis of phenotypic traits of grain showed that TKW of TaCwi-A1a was significantly higher than TaCwi-A1b in released varieties,region test varieties and breeding varieties.Similarly,KL and KW of TaCwi-A1a were also significantly higher than TaCwi-A1b.This study further verified the additive effect of TaCwi-A1a on phenotypic traits of grain,which showed that TaCwi-A1a was superior allelic variation for the grain weight and its constituent elements.In addition,the study identified the distribution of TaCwi-A1 in Yellow and Huai River Valleys Facultative Wheat Zone in China,and provided useful information for the marker-assisted selection of wheat.

Key words: Common wheat, TaCwi-A1, Molecular marker, Allelic variation, Thousand kernel weight, Kernel length, Kernel width

中图分类号:

S512.01

刘永伟, 周硕, 王雪征, 孙果忠, 朱金永, 韩秋芬, 李春杰, 赵和, 王海波. 粒重基因TaCwi-A1等位变异在黄淮麦区小麦品种(系)中的分布及功能分析[J]. 华北农学报, 2017, 32(2): 131-137. doi: 10.7668/hbnxb.2017.02.020.

LIU Yongwei, ZHOU Shuo, WANG Xuezheng, SUN Guozhong, ZHU Jinyong, HAN Qiufen, LI Chunjie, ZHAO He, WANG Haibo. Functional Analysis and Distribution of Allelic Variations of TaCwi-A1 Gene Related to Kernel Weight in Yellow and Huai River Valleys Facultative Wheat Zone[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 131-137. doi: 10.7668/hbnxb.2017.02.020.

http://www.hbnxb.net/CN/Y2017/V32/I2/131

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粒重基因TaCwi-A1等位变异在黄淮麦区小麦品种(系)中的分布及功能分析

Functional Analysis and Distribution of Allelic Variations of TaCwi-A1 Gene Related to Kernel Weight in Yellow and Huai River Valleys Facultative Wheat Zone

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粒重基因TaCwi-A1等位变异在黄淮麦区小麦品种(系)中的分布及功能分析

Functional Analysis and Distribution of Allelic Variations of TaCwi-A1 Gene Related to Kernel Weight in Yellow and Huai River Valleys Facultative Wheat Zone

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