北方冬麦区小麦春化基因的组成与分布及其与冬春性的关系

doi:10.7668/hbnxb.20193068

华北农学报 ›› 2022, Vol. 37 ›› Issue (5): 62-67. doi: 10.7668/hbnxb.20193068

所属专题： 小麦；生物技术；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

北方冬麦区小麦春化基因的组成与分布及其与冬春性的关系

赵杰¹, 刘洪泉², 赵芸¹, 杨锴¹^,³, 傅永斌⁴, 顾玉章⁴, 孙丽静¹, 胡梦芸¹, 李辉¹^,, 张颖君¹^,

¹ 河北省农林科学院粮油作物研究所,河北省作物遗传育种实验室,河北石家庄 050035
² 河北宏瑞种业有限公司,河北石家庄 050035
³ 河北工程大学园林与生态工程学院,河北邯郸 056038
⁴ 张家口市农业科学院,河北张家口 075000

收稿日期:2022-06-26 出版日期:2022-10-28
通讯作者: 李辉, 张颖君
基金资助:
国家自然科学基金青年基金项目(32101749); 河北省农林科学院科技创新人才队伍建设项目(C21R0311); 河北省省级科技计划(C2019301135); 河北省省级科技计划(20326345D); 现代种业科技创新专项——小麦现代种业科技创新团队(21326318D); 河北省现代农业产业技术体系项目(HBCT2018010201); 河北省农林科学院科技创新专项课题(2022KJCXZX-LYS-1)

Composition and Distribution of Wheat VRN Genes and Their Relationship with Vernalization in the Northern China Winter Wheat Region

ZHAO Jie¹, LIU Hongquan², ZHAO Yun¹, YANG Kai¹^,³, FU Yongbin⁴, GU Yuzhang⁴, SUN Lijing¹, HU Mengyun¹, LI Hui¹^,, ZHANG Yingjun¹^,

¹ Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences, Hebei Provincial Laboratory of Crop Genetics and Breeding,Shijiazhuang 050035,China
² Hebei Hongrui Seed Limited Company,Shijiazhuang 050035,China
³ School of Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056038,China
⁴ Zhangjiakou Academy of Agriculture Sciences,Zhangjiakou 075000,China

Received:2022-06-26 Published:2022-10-28
Contact: LI Hui, ZHANG Yingjun

摘要/Abstract

摘要：

为探究北方冬麦区小麦品种春化基因组成与冬春性的关系,以北方冬麦区271份小麦主栽品种为试验材料,通过分子标记对4个主效春化基因Vrn-A1、Vrn-B1、Vrn-D1和Vrn-B3的组成和分布进行检测,并在海南省三亚市观察田间抽穗情况,同时结合资料记载调查其冬春性特性。分子标记检测结果显示,4个显性等位基因中Vrn-D1(占27.7%)在供试品种中分布频率最高,且呈现由南向北逐渐降低趋势;显性等位基因Vrn-B1在该麦区小麦材料中分布频率较低(占3.0%);所检测材料中均不含显性等位基因Vrn-A1和Vrn-B3;该麦区小麦春化基因组合主要为vrn-A1/vrn-B1/vrn-D1/vrn-B3。通过对供试小麦材料进行冬春性分析,发现部分含有显性等位基因Vrn-D1的材料需要春化才能开花结实,而包含显性等位基因Vrn-B1的小麦材料对春化的需求明显较弱。4个显性春化基因中,只有显性等位基因Vrn-D1和Vrn-B1在所检测的北方冬麦区小麦品种中有分布,且显性等位基因Vrn-B1对小麦春化发育特性的作用强于Vrn-D1。

关键词: 普通小麦, 春化基因, 冬春性

Abstract:

In order to explore the relationship between the vernalization genes and winter-spring characteristics of wheat varieties in the Northern China Winter Wheat Region,271 wheat cultivars from the Northern China Winter Wheat Region were used as materials to detect the composition and distribution of four vernalization genes Vrn-A1,Vrn-B1,Vrn-D1 and Vrn-B3 by molecular markers.The heading date of these cultivars in the field was observed in Sanya,Hainan Province,and their winter-spring characteristics were investigated with the data recorded.The results showed that in this wheat region:among the four dominant alleles,Vrn-D1 (27.7%)had the highest distribution frequency in the tested cultivars and the distribution frequency decreased gradually from the South to the North;the distribution frequency of dominant Vrn-B1 alleles (3.0%)was very low in wheat materials in this wheat region;none of the test materials contained the dominant alleles Vrn-A1 and Vrn-B3;the most common vernalization gene allele combination was vrn-A1/vrn-B1/vrn-D1/vrn-B3.Further analysis of winterness-springness type of the test cultivars showed that some cultivars containing the dominant allele Vrn-D1 must be vernalized to blossom,while the wheat cultivars containing the dominant allele Vrn-B1 had a weak demand for vernalization.Among the four dominant vernalization genes,only the dominant alleles Vrn-D1 and Vrn-B1 were distributed in the tested cultivars in the Northern China Winter Wheat Region.The effect of Vrn-B1 on the vernalization development characteristics was stronger than that of Vrn-D1.

Key words: Common wheat, Vernalization gene, Winterness-springness type

赵杰, 刘洪泉, 赵芸, 杨锴, 傅永斌, 顾玉章, 孙丽静, 胡梦芸, 李辉, 张颖君. 北方冬麦区小麦春化基因的组成与分布及其与冬春性的关系[J]. 华北农学报, 2022, 37(5): 62-67. doi: 10.7668/hbnxb.20193068.

ZHAO Jie, LIU Hongquan, ZHAO Yun, YANG Kai, FU Yongbin, GU Yuzhang, SUN Lijing, HU Mengyun, LI Hui, ZHANG Yingjun. Composition and Distribution of Wheat VRN Genes and Their Relationship with Vernalization in the Northern China Winter Wheat Region[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 62-67. doi: 10.7668/hbnxb.20193068.

http://www.hbnxb.net/CN/Y2022/V37/I5/62

图/表 4

参考文献 25

[1]	王东, 王崇宇, 徐勤省, 王洪刚, 李兴锋. 小麦新种质系SN0594春化和光周期反应特性鉴定[J]. 山东农业科学, 2020, 52(4):1-9.doi: 10.14083/j.issn.1001-4942.2020.04.001. doi: 10.14083/j.issn.1001-4942.2020.04.001
	Wang D, Wang C Y, Xu Q X, Wang H G, Li X F. Analysis of vernalization and photoperiod response of new wheat germplasm SN0594[J]. Shandong Agricultural Sciences, 2020, 52(4):1-9.
[2]	Xu S J, Dong Q, Deng M, Lin D X, Xiao J, Cheng P L, Xing L J, Niu Y D, Gao C X, Zhang W H, Xu Y Y, Chong K. The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat[J]. Molecular Plant, 2021, 14(9):1525-1538.doi: 10.1016/j.molp.2021.05.026. doi: 10.1016/j.molp.2021.05.026 URL
[3]	陈满霞, 蒋玉蓉, 於金生. 小麦春化作用研究进展[J]. 江苏农业科学, 2019, 47(24):6-12.doi: 10.15889/j.issn.1002-1302.2019.24.002. doi: 10.15889/j.issn.1002-1302.2019.24.002
	Chen M X, Jiang Y R, Yu J S. Research progress of vernalization of wheat[J]. Jiangsu Agricultural Sciences, 2019, 47(24):6-12.
[4]	冯岳, 褚蔚, 隋新霞, 黄承彦, 崔德周, 樊庆琦, 楚秀生. 小麦冬春性人工低温春化鉴定的研究[J]. 山东农业科学, 2018, 50(1):9-15.doi: 10.14083/j.issn.1001-4942.2018.01.002. doi: 10.14083/j.issn.1001-4942.2018.01.002
	Feng Y, Chu W, Sui X X, Huang C Y, Cui D Z, Fan Q Q, Chu X S. Research on identification of winterness and springness of wheat varieties through the artificial vernalization method[J]. Shandong Agricultural Sciences, 2018, 50(1):9-15.
[5]	Berezhnaya A, Kiseleva A, Leonova I, Salina E. Allelic variation analysis at the vernalization response and photoperiod genes in Russian wheat varieties identified two novel alleles of Vrn-B3[J]. Biomolecules, 2021, 11(12):1897.doi: 10.3390/biom11121897. doi: 10.3390/biom11121897 URL
[6]	Yan L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J. Positional cloning of the wheat vernalization gene VRN1[J]. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(10):6263-6268.doi: 10.1073/pnas.0937399100. doi: 10.1073/pnas.0937399100 pmid: 12730378
[7]	Yan L L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, SanMiguel P, Bennetzen J L, Echenique V, Dubcovsky J. The wheat VRN2 gene is a flowering repressor down-regulated by vernalization[J]. Science, 2004, 303(5664):1640-1644.doi: 10.1126/science.1094305. doi: 10.1126/science.1094305 URL
[8]	Kippes N, Chen A, Zhang X Q, Lukaszewski A J, Dubcovsky J. Development and characterization of a spring hexaploid wheat line with no functional VRN2 genes[J]. Theoretical and Applied Genetics, 2016, 129(7):1417-1428.doi: 10.1007/s00122-016-2713-3. doi: 10.1007/s00122-016-2713-3 URL
[9]	Yan L, Fu D, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Yasuda S, Dubcovsky J. The wheat and barley vernalization gene VRN3 is an orthologue of FT[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(51):19581-19586.doi: 10.1073/pnas.0607142103. doi: 10.1073/pnas.0607142103 pmid: 17158798
[10]	Kippes N, Zhu J, Chen A, Vanzetti L, Lukaszewski A, Nishida H, Kato K, Dvorak J, Dubcovsky J. Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat[J]. Molecular Genetics and Genomics, 2014, 289(1):47-62.doi: 10.1007/s00438-013-0788-y. doi: 10.1007/s00438-013-0788-y URL
[11]	Sun Q M, Zhou R H, Gao L F, Zhao G Y, Jia J Z. The characterization and geographical distribution of the genes responsible for vernalization requirement in Chinese bread wheat[J]. Journal of Integrative Plant Biology, 2009, 51(4):423-432.doi: 10.1111/j.1744-7909.2009.00812.x. doi: 10.1111/j.1744-7909.2009.00812.x URL
[12]	Xu S J, Chong K. Remembering winter through vernalisation[J]. Nature Plants, 2018, 4(12):997-1009.doi: 10.1038/s41477-018-0301-z. doi: 10.1038/s41477-018-0301-z pmid: 30478363
[13]	郭阳阳, 张博, 范其茹, 郝喜英, 田淑媛, 张晓科, 杨松杰. 小麦春化基因新等位变异Vrn-B3d的鉴定[J]. 麦类作物学报, 2020, 40(12):1409-1415.doi: 10.7606/j.issn.1009-1041.2020.12.01. doi: 10.7606/j.issn.1009-1041.2020.12.01
	Guo Y Y, Zhang B, Fan Q R, Hao X Y, Tian S Y, Zhang X K, Yang S J. Characterization of a novel vernalization allele Vrn-B3d in wheat[J]. Journal of Triticeae Crops, 2020, 40(12):1409-1415.
[14]	姜莹, 黄林周, 胡银岗. 中国小麦地方品种春化基因的分布及其与冬春性的关系[J]. 中国农业科学, 2010, 43(13):2619-2632.doi: 10.3864/j.issn.0578-1752.2010.13.002. doi: 10.3864/j.issn.0578-1752.2010.13.002
	Jiang Y, Huang L Z, Hu Y G. Distribution of vernalization genes in Chinese wheat landraces and their relationship with winter hardness[J]. Scientia Agricultura Sinica, 2010, 43(13):2619-2632.
[15]	马丽娟, 王翔, 卫丽, 冯雅岚, 任江萍, 尹钧. 不同发育特性小麦品种春化基因VRN2的序列分析[J]. 麦类作物学报, 2012, 32(4):603-609.doi: 10.7606/j.issn.1009-1041.2012.04.002. doi: 10.7606/j.issn.1009-1041.2012.04.002
	Ma L J, Wang X, Wei L, Feng Y L, Ren J P, Yin J. Sequences analysis of the vernalization gene VRN2 in different development characteristic common wheat (Triticum aestivum L.)[J]. Journal of Triticeae Crops, 2012, 32(4):603-609.
[16]	买春艳, 李洪杰, 刘宏伟, 杨丽, 于立强, 周阳, 张宏军. 北方冬麦区小麦品种产量相关性状和幼穗分化特点研究[J]. 麦类作物学报, 2018, 38(7):773-781.doi: 10.7606/j.issn.1009-1041.2018.07.03. doi: 10.7606/j.issn.1009-1041.2018.07.03
	Mai C Y, Li H J, Liu H W, Yang L, Yu L Q, Zhou Y, Zhang H J. Characterization of yield related traits and inflorescence differentiation in representative wheat cultivars from the Northern China winter wheat region[J]. Journal of Triticeae Crops, 2018, 38(7):773-781.
[17]	Guo J H, Bai X H, Shi W P, Li R J, Hao X Y, Wang H F, Gao Z Q, Guo J, Lin W. Risk assessment of freezing injury during overwintering of wheat in the northern boundary of the Winter Wheat Region in China[J]. PeerJ, 2021, 9:e12154.doi: 10.7717/peerj.12154. doi: 10.7717/peerj.12154 URL
[18]	马倩倩. 未来气候变暖情景下北部冬麦区冬小麦生育期演变趋势预测分析[J]. 麦类作物学报, 2021, 41(6):762-770.doi: 10.7606/j.issn.1009-1041.2021.06.15. doi: 10.7606/j.issn.1009-1041.2021.06.15
	Ma Q Q. Analysis of winter wheat phenology evolution in Northern China wheat zone under global warming[J]. Journal of Triticeae Crops, 2021, 41(6):762-770.
[19]	孟繁圆, 冯利平, 张丰瑶, 张祎, 伍露, 王春雷, 闫锦涛, 彭明喜, 莫志鸿, 余卫东. 北部冬麦区冬小麦越冬冻害时空变化特征[J]. 作物学报, 2019, 45(10):1576-1585.doi: 10.3724/SP.J.1006.2019.81076. doi: 10.3724/SP.J.1006.2019.81076
	Meng F Y, Feng L P, Zhang F Y, Zhang Y, Wu L, Wang C L, Yan J T, Peng M X, Mo Z H, Yu W D. Temporal and spatial variations of winter wheat freezing injury in northern winter wheat region[J]. Acta Agronomica Sinica, 2019, 45(10):1576-1585.
[20]	Yan L, Helguera M, Kato K, Fukuyama S, Sherman J, Dubcovsky J. Allelic variation at the VRN-1 promoter region in polyploid wheat[J]. Theoretical and Applied Genetics, 2004, 109(8):1677-1686.doi: 10.1007/s00122-004-1796-4. doi: 10.1007/s00122-004-1796-4 pmid: 15480533
[21]	Fu D L, Szucs P, Yan L L, Helguera M, Skinner J S, von Zitzewitz J, Hayes P M, Dubcovsky J. Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat[J]. Molecular Genetics and Genomics, 2005, 273(1):54-65.doi: 10.1007/s00438-004-1095-4. doi: 10.1007/s00438-004-1095-4 URL
[22]	李哲, 杨杰, 李瑞博, 王晓龙, 张晓科, 张玲丽. 中国小麦主产区品种主要春化基因的组成与分布[J]. 西北农林科技大学学报(自然科学版), 2018, 46(9):35-40.doi: 10.13207/j.cnki.jnwafu.2018.09.005. doi: 10.13207/j.cnki.jnwafu.2018.09.005
	Li Z, Yang J, Li R B, Wang X L, Zhang X K, Zhang L L. Composition and distribution of major vernalization genes in wheat cultivars from main production areas in China[J]. Journal of Northwest A&F University (Natural Science Edition), 2018, 46(9):35-40.
[23]	郭总总, 王翔, 卫丽, 白瑞英, 曹云, 郭创, 尹钧. 黄淮麦区小麦春化基因组成的多态性分布研究[J]. 河南农业大学学报, 2014, 48(3):255-262.doi: 10.16445/j.cnki.1000-2340.2014.03.010. doi: 10.16445/j.cnki.1000-2340.2014.03.010
	Guo Z Z, Wang X, Wei L, Bai R Y, Cao Y, Guo C, Yin J. The polymorphism distribution of the vernalization genes in wheat cultivars in Huang-Huai wheat region[J]. Journal of Henan Agricultural University, 2014, 48(3):255-262.
[24]	游银, 王晓翠, 杨超凡, 陈晓杰, 卢山, 吉万全, 闵东红, 孙道杰, 胡银岗, 陈亮. 黄淮麦区部分骨干品种重要基因等位类型分析及全基因组优异位点挖掘[J]. 麦类作物学报, 2019, 39(11):1265-1275.doi: 10.7606/j.issn.1009-1041.2019.11.01. doi: 10.7606/j.issn.1009-1041.2019.11.01
	You Y, Wang X C, Yang C F, Chen X J, Lu S, Ji W Q, Min D H, Sun D J, Hu Y G, Chen L. Allelic type analysis of important genes in key varieties from Huanghuai wheat area and exploration of its elite alleles in the whole genome[J]. Journal of Triticeae Crops, 2019, 39(11):1265-1275.
[25]	赵彦坤, 张文胜, 王秀堂, 傅晓艺, 贾丹, 高振贤, 史占良, 何明琦. 黄淮北片主栽小麦品种冬春性与主要春化基因组成的关系[J]. 麦类作物学报, 2016, 36(11):1440-1448.doi: 10.7606/j.issn.1009-1041.2016.11.05. doi: 10.7606/j.issn.1009-1041.2016.11.05
	Zhao Y K, Zhang W S, Wang X T, Fu X Y, Jia D, Gao Z X, Shi Z L, He M Q. Analysis of winterness-springness type and vernalization genes in the main wheat cultivars from the North of Yellow and Huai valley of China[J]. Journal of Triticeae Crops, 2016, 36(11):1440-1448.

位点 Locus	等位基因 Allele	引物名称 Primer name	引物序列(5'—3') Sequence(5'—3')	扩增产物/bp PCR product	退火温度/℃ Annealing temperature	参考文献 Reference
Vrn-A1	Vrn-A1a	VRN1AF	GAAAGGAAAAATTCTGCTCG	965+876	50.0	[20]
	Vrn-A1b			714
	vrn-A1、	VRN1-INT1R	GCAGGAAATCGAAATCGAAG	734
	Vrn-A1c
	Vrn-A1c	Intr1/A/F2	AGCCTCCACGGTTTGAAAGTAA	1 170	58.9	[21]
		Intr1/A/R3	AAGTAAGACAACACGAATGTGAGA
	vrn-A1	Intr1/C/F	GCACTCCTAACCCACTAACC	1 068	56.0	[21]
		Intr1/AB/R	TCATCCATCATCAAGGCAAA
Vrn-B1	Vrn-B1	Intr/B/F	CAAGTGGAACGGTTAGGACA	709	58.0	[21]
		Intr1/B/R3	CTCATGCCAAAAATTGAAGATGA
	vrn-B1	Intr/B/F	CAAGTGGAACGGTTAGGACA	1 149	56.4	[21]
		Intr1/B/R4	CAAATGAAAAGGAATGAGAGCA
Vrn-D1	Vrn-D1	Intr/D/F	GTTGTCTGCCTCATCAAATCC	1 671	61.0	[21]
		Intr1/D/R3	GGTCACTGGTGGTCTGTGC
	vrn-D1	Intr/D/F	GTTGTCTGCCTCATCAAATCC	997	61.0	[21]
		Intr1/D/R4	AAATGAAAAGGAACGAGAGCG
Vrn-B3	Vrn-B3	VRN4-B-INS-F	CATAATGCCAAGCCGGTGAGTAC	1 200	63.0	[9]
		VRN4-B-INS-R	ATGTCTGCCAATTAGCTAGC
	vrn-B3	VRN4-B-NOINS-F	ATGCTTTCGCTTGCCATCC	1 140	57.0	[9]
		VRN4-B-NOINS-R	CTATCCCTACCGGCCATTAG

位点 Locus	等位基因 Allele	引物名称 Primer name	引物序列(5'—3') Sequence(5'—3')	扩增产物/bp PCR product	退火温度/℃ Annealing temperature	参考文献 Reference
Vrn-A1	Vrn-A1a	VRN1AF	GAAAGGAAAAATTCTGCTCG	965+876	50.0	[20]
	Vrn-A1b			714
	vrn-A1、	VRN1-INT1R	GCAGGAAATCGAAATCGAAG	734
	Vrn-A1c
	Vrn-A1c	Intr1/A/F2	AGCCTCCACGGTTTGAAAGTAA	1 170	58.9	[21]
		Intr1/A/R3	AAGTAAGACAACACGAATGTGAGA
	vrn-A1	Intr1/C/F	GCACTCCTAACCCACTAACC	1 068	56.0	[21]
		Intr1/AB/R	TCATCCATCATCAAGGCAAA
Vrn-B1	Vrn-B1	Intr/B/F	CAAGTGGAACGGTTAGGACA	709	58.0	[21]
		Intr1/B/R3	CTCATGCCAAAAATTGAAGATGA
	vrn-B1	Intr/B/F	CAAGTGGAACGGTTAGGACA	1 149	56.4	[21]
		Intr1/B/R4	CAAATGAAAAGGAATGAGAGCA
Vrn-D1	Vrn-D1	Intr/D/F	GTTGTCTGCCTCATCAAATCC	1 671	61.0	[21]
		Intr1/D/R3	GGTCACTGGTGGTCTGTGC
	vrn-D1	Intr/D/F	GTTGTCTGCCTCATCAAATCC	997	61.0	[21]
		Intr1/D/R4	AAATGAAAAGGAACGAGAGCG
Vrn-B3	Vrn-B3	VRN4-B-INS-F	CATAATGCCAAGCCGGTGAGTAC	1 200	63.0	[9]
		VRN4-B-INS-R	ATGTCTGCCAATTAGCTAGC
	vrn-B3	VRN4-B-NOINS-F	ATGCTTTCGCTTGCCATCC	1 140	57.0	[9]
		VRN4-B-NOINS-R	CTATCCCTACCGGCCATTAG

省份 Province	Vrn-B1 品种数 Number of varieties	Vrn-B1 频率/% Frequency	Vrn-D1 品种数 Number of varieties	Vrn-D1 频率/% Frequency
北京 Beijing	0	0.0	1	7.1
天津 Tianjin	0	0.0	0	0.0
河北 Hebei	0	0.0	30	38.0
河南 Henan	3	4.2	22	31.0
山东 Shandong	2	3.5	6	10.5
山西 Shanxi	0	0.0	3	12.5
陕西 Shaanxi	3	20.0	5	33.3
甘肃 Gansu	0	0.0	0	0.0
江苏 Jiangsu	0	0.0	8	88.9
总计 Total	8	3.0	75	27.7

省份 Province	Vrn-B1 品种数 Number of varieties	Vrn-B1 频率/% Frequency	Vrn-D1 品种数 Number of varieties	Vrn-D1 频率/% Frequency
北京 Beijing	0	0.0	1	7.1
天津 Tianjin	0	0.0	0	0.0
河北 Hebei	0	0.0	30	38.0
河南 Henan	3	4.2	22	31.0
山东 Shandong	2	3.5	6	10.5
山西 Shanxi	0	0.0	3	12.5
陕西 Shaanxi	3	20.0	5	33.3
甘肃 Gansu	0	0.0	0	0.0
江苏 Jiangsu	0	0.0	8	88.9
总计 Total	8	3.0	75	27.7

显性等位基因 Dominant allele	春性品种数 Number of spring varieties	春性品种频率/% Frequency of spring varieties	弱春性品种数 Number of weak spring varieties	弱春性品种频率/% Frequency of weak spring varieties	弱冬性品种数 Number of weak winter varieties	弱冬性品种频率/% Frequency of weak winter varieties	半冬性品种数 Number of semi-winter varieties	半冬性品种频率/% Frequency of semi- winter varieties	冬性品种数 Number of winter varieties	冬性品种频率/% Frequency of winter varieties	总计 Total
Vrn-B1	3	37.5	2	25.0	0	0.0	3	37.5	0	0.0	8
Vrn-D1	6	8.0	7	9.3	1	1.3	53	70.7	8	10.7	75

选择文件类型/文献管理软件名称

选择包含的内容

北方冬麦区小麦春化基因的组成与分布及其与冬春性的关系

Composition and Distribution of Wheat VRN Genes and Their Relationship with Vernalization in the Northern China Winter Wheat Region

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 25

相关文章 15

编辑推荐

Metrics

本文评价

[1]	兰素缺, 李光威, 孟雅宁, 李芳, 张业伦, 李杏普. *小麦抗白粉病基因pm30种质改良及鉴定*[J]. 华北农学报, 2017, 32(S1): 7-11.
[2]	李强, 李丁, 乔文臣, 孙书娈, 孟祥海, 魏建伟, 赵明辉, 赵凤梧, 李会敏. 衡观35广适基因对冬春小麦杂种优势利用初探[J]. 华北农学报, 2017, 32(S1): 220-225.
[3]	刘永伟, 周硕, 王雪征, 孙果忠, 朱金永, 韩秋芬, 李春杰, 赵和, 王海波. *粒重基因TaCwi-A1等位变异在黄淮麦区小麦品种(系)中的分布及功能分析*[J]. 华北农学报, 2017, 32(2): 131-137.
[4]	张宝娜, 朱灿灿, 张鹏钰, 卫丽, 王翔, 姜玉梅, 王同朝. *普通小麦春化基因VRN1* RNA干扰载体构建及转基因小麦获得**[J]. 华北农学报, 2016, 31(1): 57-62.
[5]	沈红霞, 辛明明, 梁荣奇, 解超杰, 杨作民, 刘志勇, 孙其信. 野生二粒小麦导入普通小麦抗白粉病基因的分子标记[J]. 华北农学报, 2012, 27(4): 7-11.
[6]	孙果忠, 游光霞, 武淑祯, 张秀英, 王海波, 肖世和. 小麦春化基因的遗传效应研究[J]. 华北农学报, 2011, 26(6): 1-5.
[7]	杨松杰, 张晶, 张晓科. 基于PCR标记的陕西省部分地方小麦品种(系)糯蛋白基因的多样性分析[J]. 华北农学报, 2011, 26(6): 67-72.
[8]	田齐建, 李晓燕, 王曙光, 孙黛珍. Presto×晋农190杂种F₂-3及双亲的核型分析[J]. 华北农学报, 2010, 25(6): 92-96.
[9]	杨松杰, 张影全, 王茹, 张晓科. 陕西省地方小麦品种黄色素含量基因的遗传分析[J]. 华北农学报, 2010, 25(4): 83-87.
[10]	王长有, 王秋英, 刘志立, 张宏, 王亚娟, 刘新伦, 吉万全. 小麦新种质N95175抗白粉病基因的染色体定位[J]. 华北农学报, 2010, 25(1): 213-216.
[11]	程敦公, 李豪圣, 肖永贵, 宋健民, 刘爱峰, 刘建军. 快速准确鉴定小麦1BL/1RS易位系新方法的研究[J]. 华北农学报, 2009, 24(S1): 34-37.
[12]	王美芳, 雷振生, 何中虎, 孙其信, 吴政卿, 何盛莲, 杨攀. 冬小麦品种的HMW－GS组成和1BL／1RS易位分布及其与品质性状的关系研究[J]. 华北农学报, 2008, 23(5): 62-66.
[13]	王世杰, 李保云, 康明辉, 刘春雷, 达博雯, 林作楫, 尤明山, 刘广田. 细胞质对普通小麦面包加工品质的影响[J]. 华北农学报, 2008, 23(4): 217-222.
[14]	桑利群, 王长有, 王秋英, 吉万全. 小麦种质N9659抗白粉病基因SSR标记研究[J]. 华北农学报, 2008, 23(3): 162-165.
[15]	康国章, 肖向红, 王永华, 郭天财, 朱云集, 官春云. 小麦淀粉GBSSII基因的克隆、反义和RNAi载体的构建[J]. 华北农学报, 2008, 23(1): 7-11.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

北方冬麦区小麦春化基因的组成与分布及其与冬春性的关系

Composition and Distribution of Wheat VRN Genes and Their Relationship with Vernalization in the Northern China Winter Wheat Region

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 25

相关文章 15

编辑推荐

Metrics

本文评价