华北农学报

所属专题: 小麦

• 论文 • 上一篇    下一篇

快速准确鉴定小麦1BL/1RS易位系新方法的研究

程敦公1, 李豪圣1, 肖永贵2, 宋健民1, 刘爱峰1, 刘建军1   

  1. 1. 山东农业科学院作物研究所, 山东济南 250100;
    2. 中国农业科学院作物科学研究所, 国家小麦改良中心, 国家农作物基因资源与基因改良重大科学工程, 北京 100081
  • 收稿日期:2009-03-02 出版日期:2009-12-31
  • 通讯作者: 刘建军(1963 -), 男, 山东威海人, 研究员, 主要从事小麦遗传育种和品质理论研究。
  • 作者简介:程敦公(1979 -), 男, 山东泰安人, 助理研究员, 主要从事小麦品质遗传育种研究。刘爱峰(1969 -), 女, 山东青州人, 博士, 副研究员, 主要从事小麦品质遗传育种研究工作。
  • 基金资助:
    国家科技支撑计划项目(2006BAD01A02-14);国家公益性行业(农业)科研专项经费项目(nyhyzx07-002);农业部引进国际先进农业科学技术计划(“948”计划)项目(2006-G2);山东省三0工程项目;山东省泰山学者项目

New Method to Identify 1BL/1RS Translocation in Common Wheat

CHENGDun-gong1, LIHao-sheng1, XIAOYong-gui2, SONGJian-min1, LIUAi-feng1, LIUJian-jun1   

  1. 1. Crop Research Institute, Shandong Academy of Agriculture Sciences, Jinan 250100, China;
    2. Institute of Crop Sciences, National Wheat Improvement Centre, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agriculture Sciences, Beijing 100081, China
  • Received:2009-03-02 Published:2009-12-31

摘要: 利用404份高代品系(试验Ⅰ)和175份山东省主栽品种及高代品系(试验Ⅱ),研究了1BL/1RS易位对小麦揉面参数的影响,探讨利用揉面特性鉴定1BL/1RS易位系的方法。结果表明,1BL/1RS易位导致小麦的揉面特性显著变劣,1BL/1RS易位系的揉面时间、峰值带宽及峰后1 min带宽显著低于非1BL/1RS易位系,而衰落角和带宽比(峰值带宽/峰后1 min带宽)显著高于非1BL/1RS易位系。易位系的揉面谱带的主要特征为峰后1 min谱带急剧衰落并变窄,带宽比显著增大。带宽比1.6可作为判断易位系的有效指标,即大于或等于1.6为1BL/1RS易位系,小于1.6为非1BL/1RS易位系,准确率达85.2%(试验Ⅰ)和96.8%(试验Ⅱ)。因此,揉面图谱可作为快速有效鉴别1BL/1RS易位系的新方法。

关键词: 普通小麦, 1BL/1RS易位, 揉面特性

Abstract: To develop a new method for 1BL/1RS translocation identification with mixograph parameters,404 advanced lines(Experiment I)and 175main cultivars of Shandong Province and advanced lines(Experiment II)were used in this study.The genetic effect of 1BL/1RS translocation on mixograph parameters was investigated .1BL/1RS translocation lines showed significantly shorter mixing time,less bandwidth of peak and bandwidth after 1 min peak,and higher angle of descent and the bandwidth ratio(the ratio of bandwidth of peak/bandwidth after 1 min peak)in comparison with non -1BL/1RS translocation lines .It indicated that the 1BL/1RS translocation has deleterious effects on mixograph parameters . Mixograph of the 1BL/1RS translocation was characterized with the bandwidth sharply declining and narrowing after 1min peak,and increasing the bandwidth ratio ,whereas the bandwidth of non -1BL/1RS translocations declined gently after 1 min peak or had a longer mixing tolerance,and had a little variation about the bandwidth ratio .Furthermore,85 .2 %(Experiment I)and 96 .8 %(Experiment II)accuracies were achieved in grouping the 1BL/1RS translocation and non -1BL/1RS translocation on the basis of the band width ratio,i .e ., 1BL/1RS translocation line had a value more than or equal to 1 .6,and non -1BL/1RS translocation line had a value smaller than 1 .6 .Therefore ,mixogram and its parameters could be used as a new method to determine the presence of 1BL/1RS translocation quickly and efficiently.

Key words: Commonwheat(TriticumaestivumL.), 1BL/1RStranslocation, Mixogramcharacteristics

中图分类号: 

引用本文

CHENGDun-gong, LIHao-sheng, XIAOYong-gui, SONGJian-min, LIUAi-feng, LIUJian-jun. New Method to Identify 1BL/1RS Translocation in Common Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.009.

使用本文

0
    /   /   推荐 /   导出引用

链接本文: http://www.hbnxb.net/CN/10.7668/hbnxb.2009.S1.009

               http://www.hbnxb.net/CN/Y2009/V24/IS1/34