基于PCR标记的陕西省部分地方小麦品种(系)糯蛋白基因的多样性分析

doi:10.7668/hbnxb.2011.06.013

摘要/Abstract

摘要： 利用3个优化的STS标记对陕西省1960年以来引进和自育的99份小麦品种(系)的Waxy蛋白基因的变异情况进行了鉴定。结果表明,Wx-A1位点的标记在Wx-A1a和Wx-A1b基因型内分别扩增出336 bp和317 bp特异片段;Wx-B1a基因型内扩增出425 bp特异产物,Wx-B1b基因型内不扩增出此产物;Wx-D1a和Wx-D1b基因型内分别扩增出867 bp和279 bp特异产物。检测出除陕糯1号基因型为Wx-A1b+Wx-B1b+Wx-D1b外,Wx-B1b基因缺失突变材料12份,无Wx-A1b基因和Wx-D1b基因缺失突变材料。这3个标记可以用于分子标记辅助育种,并提供了部分品种的缺失类型。

关键词: 普通小麦, 地方品种, Waxy蛋白基因, PCR标记, 多样性

Abstract: Three optimized STS markers were used to analyze 99 wheat cultivars in Shaanxi from 1960 with different types of Waxy proteins． The results indicated that the marker on Wx-A1 locus amplified 336 bp and 317 bp special fragments associated to Wx-A1aand Wx-A1b genotypes; the marker on Wx-B1 amplified 425 bp special fragment associated to Wx-B1a and null special fragment associated to Wx-A1b genotype and the marker on Wx-D1 amplified 867 bp and 279 bp special fragments associated to Wx-D1aand Wx-D1bgenotypes， respectively． Except for the cultivar Shaannuo 1 with the genotype of Wx-A1b + Wx-B1b + Wx-D1b，12 cultivars were identified to null Wx-B1 mutants and no cultivars were detected for null Wx-A1 mutants and null Wx-D1 mutants． The three optimized STS markers were demonstrated to be useful tools to identify wheat cultivars with mutant and normal alleles of the Waxy genes in marker-assisted selection of wheat breeding programs and the genotypes of Waxy genes of some materials were released in this paper．

Key words: Bread wheat(Triticum aestivumL．), Landrace, Waxy protein gene, Molecular marker, Diversity

中图分类号:

杨松杰, 张晶, 张晓科. 基于PCR标记的陕西省部分地方小麦品种(系)糯蛋白基因的多样性分析[J]. 华北农学报, 2011, 26(6): 67-72. doi: 10.7668/hbnxb.2011.06.013.

YANG Song-jie, ZHANG Jing, ZHANG Xiao-ke. A PCR-Based Diversity Analysis of Waxy Genes in Shaanxi Wheat Cultivars and Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 67-72. doi: 10.7668/hbnxb.2011.06.013.

http://www.hbnxb.net/CN/Y2011/V26/I6/67

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