茶与大豆间作对土壤微生物群落代谢功能多样性的影响

doi:10.7668/hbnxb.20191878

摘要/Abstract

摘要： 探究茶与大豆间作条件下根系土壤微生物群落代谢功能多样性的变化特征，有助于进一步了解茶园间作生态系统高产优质的机制。通过田间试验，设置茶树单作、大豆单作和茶树/大豆间作3种种植模式，运用Biolog技术分析土壤微生物群落代谢功能多样性的变化。结果表明，与单作模式相比，茶-大豆间作提高了土壤微生物群落的碳源平均利用率（AWCD），在120 h分别显著提高了11.52%，12.99%。茶-大豆间作提高了土壤微生物群落对糖类、氨基酸类、羧酸类及胺类等4类碳源的利用强度，并且提高了土壤微生物多样性指数。主成分分析表明，茶树与大豆间作主要通过改变土壤微生物群落对糖类、羧酸类碳源利用的模式来改善土壤微生物群落的代谢多样性。本试验条件下，茶与大豆间作改变了根际微生物的群落组成，改善了根际微生物群落功能多样性。

关键词: 茶树, 大豆, 间作, 土壤微生物, 代谢多样性

Abstract: To explore the metabolic functional diversity of root soil microbial community in tea and soybean intercropping contributes to further understand the mechanism of high yield and good quality of tea garden. A field trial with three planting patterns(tea monocropping, soybean monocropping, tea and soybean intercropping)was conducted to analyze the changes of metabolic function diversity of soil microbial community by Biolog technology. Results showed that intercropping increased the average well color development(AWCD)of soil microbial community of tea and soybean by 11.52% and 12.99% at 120 h, respectively, compared with monocropping pattern. Intercropping enhanced the utilization of carbohydrate, amino acid, carboxylic acid and amine by soil microbial community of tea and soybean and improved the soil microbial diversity indices. Principal component analysis demonstrated that tea and soybean intercropping significantly improved the metabolic diversity of soil microbial community by changing the utilization patterns of carbohydrates and carboxylic acids by soil microorganism. Under this experiment, intercropping of tea and soybean changed the composition of rhizosphere microbial community and improved the functional diversity of microbial community.

Key words: Tea, Soybean, Intercropping, Soil microorganism, Metabolic diversity

中图分类号:

韦锦坚, 覃潇敏, 农玉琴, 骆妍妃, 陆金梅, 陈远权, 韦持章. 茶与大豆间作对土壤微生物群落代谢功能多样性的影响[J]. 华北农学报, 2021, 36(S1): 289-296. doi: 10.7668/hbnxb.20191878.

WEI Jinjian, QIN Xiaomin, NONG Yuqin, LUO Yanfei, LU Jinmei, CHEN Yuanquan, WEI Chizhang. Effects of Tea and Soybean Intercropping on Metabolic Functional Diversity of Soil Microbial Community[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 289-296. doi: 10.7668/hbnxb.20191878.

http://www.hbnxb.net/CN/Y2021/V36/IS1/289

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