基于SCoT分子标记的甘薯及其野生种遗传多样性分析

doi:10.7668/hbnxb.20191325

摘要/Abstract

摘要： 为揭示甘薯及其野生种之间的遗传多样性，分析甘薯及其野生种之间的遗传关系，利用SCoT分子标记对22份甘薯及其野生种三浅裂野牵牛和三裂叶薯的PCR扩增结果、遗传多样性、进化关系、群体结构进行分析。结果表明：43条SCoT引物共检测到278条稳定条带，每条引物可检测4~12个条带； SCoT-8、SCoT-20、SCoT-26、SCoT-36、SCoT-35 5条引物多态性丰富，可用于甘薯及其野生种研究的分子标记。基于分子标记的遗传多样性分析表明，三浅裂野牵牛的遗传多样性最高，甘薯次之，三裂叶薯最小。遗传聚类和群体遗传结构分析结果将参试22份种质材料分为三大类群，各材料归类基本和其所属物种一致，不同物种间的血缘交流少，遗传组成相对单一，比较而言，甘薯与三浅裂野牵牛之间的血缘交流比三裂叶薯之间的血缘交流多，其与三浅裂野牵牛之间的遗传关系较三裂叶薯更近。综上，研究结果初步证明SCoT分子标记在甘薯进化研究中具有潜在应用价值，同时揭示了甘薯与其近缘野生种之间的不同遗传关系，为今后甘薯育种中引入三浅裂野牵牛等野生种血缘，提高甘薯遗传多样性提供了理论支持。

关键词: 甘薯, SCoT分子标记, 遗传多样性, 遗传结构

Abstract: In order to reveal the genetic diversity of sweet potato and its wild species and analyze the genetic relationship between sweet potato and its wild species, SCoT molecular markers were used to analyze the results of PCR amplification, genetic diversity, evolutionary relationship and population structure of 22 germplasm of sweetpotato and its wild species I.trifida and I.triloba in the present study. The results showed that 278 stable bands were detected by 43 primers, 4-12 bands could be detected by each primer, and five primers with rich polymorphism were screened, which were SCoT-8, SCoT-20, SCoT-26, SCoT-36 and SCoT-35. These primers could be used in the study of sweet potato molecular markers in the future. The analysis of genetic diversity based on the results of molecular markers showed that the highest genetic diversity was in I.trifida, followed by I.batatas, and the lowest in I.triloba. According to the results of genetic cluster analysis and population genetic structure analysis, 22 germplasm were divided into three groups, and the classification of each group was basically the same as its species. There was less blood exchange between different species, and the genetic composition was relatively simple. In comparison, the blood exchange between sweet potato and I.trifida was more than that between I.triloba. In conclusion, the results of this study preliminarily proved the potential application of SCoT molecular markers in the study of sweet potato evolution, and revealed the different genetic relationships between sweet potato and its wild relatives, which provided theoretical support for introducing I. trifida and other wild species into sweet potato breeding to improve the genetic diversity of sweet potato in the future.

Key words: Sweet potato, SCoT molecular markers, Genetic diversity, Genetic structure

中图分类号:

S632.03

冯俊彦, 康乐, 郎涛, 张聪, 李明, 赵珊, 蒲志刚. 基于SCoT分子标记的甘薯及其野生种遗传多样性分析[J]. 华北农学报, 2021, 36(1): 18-26. doi: 10.7668/hbnxb.20191325.

FENG Junyan, KANG Le, LANG Tao, ZHANG Cong, LI Ming, ZHAO Shan, PU Zhigang. Genetic Diversity Analysis of Sweet Potato and Its Wild Species Using SCoT Molecular Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 18-26. doi: 10.7668/hbnxb.20191325.

http://www.hbnxb.net/CN/Y2021/V36/I1/18

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