栽培种花生含油量QTL定位与上位性互作分析

doi:10.7668/hbnxb.20191533

摘要/Abstract

摘要： 为揭示栽培种花生含油量遗传机制，以高产抗逆品种花育36号和高油品系6-13为亲本，构建了181个重组自交系（RIL，Recombinant inbred line），以F_2：7-F_2：8重组自交系为试验材料，测定在3个环境下（E1、E2、E3）的籽仁含油量，进行QTL定位和上位效应分析。结果表明，群体含油量存在超亲遗传，且符合正态分布，广义遗传率为0.55。基于复合区间作图模型共检测到5个QTL，分别位于第6，8，15，17染色体，表型变异贡献率为7.39%~17.67%。在环境E1下检测到2个QTL，qOC6和qOC8.1，表型贡献率为17.67%，9.17%；在环境E2共检测到3个位点，qOC8.2、qOC15和qOC17，表型贡献率分别为8.83%，16.53%，7.39%；在环境E3共检测到1个位点qOC15，表型贡献率为17.39%。其中仅主效位点qOC15可在2个环境下被重复检测，覆盖约2.8 Mb基因组区间，增效等位基因来自6-13。基于完备区间作图法共检测到21对上位性QTL，分别位于第1，3，5，7，8，9，10，12，13，14，15，16，17，18，19，20染色体，上位性效应对互作的表型贡献率为1.24%~3.54%，上位性QTL与环境互作的表型贡献率为0~1.67%。对主效位点qOC15进行基因功能预测，共有97个功能注释基因，主要参与细胞内相关催化反应和代谢途径，其中包括4个可能参与脂质合成、代谢和转运的候选基因。本研究定位到的5个QTL和21对上位QTL，为后续花生品质改良和相关基因克隆提供理论基础。

关键词: 花生, 重组自交系, 含油量, 数量性状位点, 基因注释

Abstract: To explore the genetic basis that modulates oil content in peanut, a recombinant inbred line (RIL) population(181 individuals) was constructed by crossing two parents(Huayu 36 and 6-13) with different oil contents. The oil content of RIL population(F_2:7-F_2:8) demonstrated normal distribution and transgressive segregations were observed in three environments (E1, E2, E3). The broad-sense heritability was 0.55. QTL mapping was performed by composite interval mapping method(CIM-ADD) and a total of 5 QTLs were mapped on chromosome 6, 8, 15 and 17, with phenotypic variance explained(PVE) of 7.39%-17.67%. In the E1, two loci, qOC6 and qOC8.1 were detected with the PVE of 17.67% and 9.17%, respectively. In the E2, there were three QTLs, qOC8.2 (PVE=8.83%), qOC15 (PVE=16.53%) and qOC17 (PVE=7.39%), mapped on chromosome 8, 15 and 17. In the E3, only one QTL, qOC15 was identified with the PVE of 17.39%. Notably the major QTL, qOC15, was expressed in two environments, spanning a nearly 2.8 Mb genomic region with 6-13 allele contributing to the trait. Additionally, epistatic interaction was analyzed by inclusive composite interval mapping method (ICIM-EPI) and 21 pairs of epistatic QTLs were detected on chromosome 1, 3, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19 and 20, with the PVE by epistatic effect of 1.24%-3.54%, and PVE by interaction effect between epistatic QTL and environment of 0-1.67%. Gene annotation was performed in the qOC15 region and resulted in 97 putative functional genes, which mostly impacts on cellular catalyst activity and metabolic process, 4 of which were predicted to be involved in lipid biosynthesis, metabolic and transport processes.These 5 additive QTLs and 21 pairs of epistatic QTLs provide a theoretical basis for both peanut quality improvement and cloning of associated genes.

Key words: Peanut, Recombinant inbred line, Oil content, Quantitative trait locus, Gene annotation

中图分类号:

S565.03

张胜忠, 胡晓辉, 苗华荣, 杨伟强, 崔凤高, 邱俊兰, 陈四龙, 张建成, 陈静. 栽培种花生含油量QTL定位与上位性互作分析[J]. 华北农学报, 2021, 36(1): 27-35. doi: 10.7668/hbnxb.20191533.

ZHANG Shengzhong, HU Xiaohui, MIAO Huarong, YANG Weiqiang, CUI Fenggao, QIU Junlan, CHEN Silong, ZHANG Jiancheng, CHEN Jing. QTL Mapping and Epistatic Interaction Analysis for Oil Content in Cultivated Peanut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 27-35. doi: 10.7668/hbnxb.20191533.

http://www.hbnxb.net/CN/Y2021/V36/I1/27

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