影响多彩花生种皮颜色的关键代谢物及ANS基因分析

doi:10.7668/hbnxb.20193143

摘要/Abstract

摘要：

为了探究影响花生种皮颜色的关键代谢物及花青素合成酶(ANS)基因,以5种不同种皮颜色的花生品种(系)为材料,对其种皮中花青素代谢物组成、ANS家族基因的表达及其相关性进行分析。结果显示,在5个花生品种(系)种皮中检测到3大类,共19种花青素糖苷类物质,其中黑种皮中最多为17种,白种皮最少仅为4种,表明随着种皮颜色的加深,花青素糖苷类物质种类也逐渐增多。对5个ANS基因表达分析,结果显示,仅有Ahy_Scaffold1g106620的表达量较高,其他4个基因基本不表达或表达量较低。且Ahy_Scaffold1g106620随着种皮颜色加深,表达量显著升高,推测Ahy_Scaffold1g106620是调控花青素合成的关键基因。进一步相关分析结果显示,Ahy_Scaffold1g106620与19种糖苷物质中的12种呈现显著正相关,相关系数为0.54~0.82;而Ahy_A02g006729仅与3种花青素类糖苷物质呈现显著相关性,其他3个基因与种皮中花青素类物质含量无关,结果表明,Ahy_Scaffold1g106620是ANS家族中影响花青素物质积累的关键调控基因。以上研究结果初步发现了影响多彩花生种皮颜色的主要代谢物及ANS家族中关键的调控基因。

关键词: 花生, 花青素, ANS基因, 代谢物, 种皮颜色

Abstract:

In order to explore the major metabolites and the key anthocyanin synthase(ANS)genes which affecting the seed testa color in peanut,here,5 peanut varieties(lines)with different seed testa color were employed to determine the main anthocyanin metabolite,expression pattern of ANS family genes as well as their correlations in seed testa. The results showed that a total of 19 anthocyanin glycosides which belonged to 3 categories were detected in the seed testa of 5 peanut varieties(lines). Meanwhile,a total of 17 anthocyanin glycosides were found in the black seed testa,while only 4 anthocyanin glycosides were detected in white seed testa,indicating that the number of anthocyanin glycosides were gradually increased accompanying the deepening of seed testa color. In addition,the expression analysis of the 5 ANS genes showed that only Ahy_Scaffold1g106620 was highly expressed,while,the other 4 genes were almost not expressed or had a low expression. Furthermore,the expression level of Ahy_Scaffold1g106620 was significantly increased accompanying the deepening of seed testa color,suggesting that Ahy_Scaffold1g106620 was a key gene in regulating of anthocyanin synthesis. Further correlation analysis revealed that Ahy_Scaffold1g106620 was significantly and positively correlated with 12 of the 19 glycosides with correlation coefficients ranged from 0.54 to 0.82. However,Ahy_A02g006729 showed significant correlation with only 3 anthocyanin glycosides,and the other 3 genes had no correlation with anthocyanin content in seed testa. These results demonstrated that Ahy_Scaffold1g106620,a member of ANS gene family,was the vital regulatory gene in accumulating anthocyanin. In summary,all these results preliminarily discovered the main metabolites and key regulatory genes of ANS family that affect the seed testa color in peanut.

Key words: Peanut, Anthocyanidin, ANS genes, Metabolite, Testa color

苏俏, 金欣欣, 李玉荣, 程增书, 宋亚辉, 杨永庆, 王瑾. 影响多彩花生种皮颜色的关键代谢物及ANS基因分析[J]. 华北农学报, 2022, 37(S1): 19-25. doi: 10.7668/hbnxb.20193143.

SU Qiao, JIN Xinxin, LI Yurong, CHENG Zengshu, SONG Yahui, YANG Yongqing, WANG Jin. Analysis of Key Metabolites and ANS Genes Affecting Seed Testa Color of Peanut[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 19-25. doi: 10.7668/hbnxb.20193143.

http://www.hbnxb.net/CN/Y2022/V37/IS1/19

图/表 5

图1 花青素在5个花生品种(系)种皮中的种类分析

Fig.1 Analysis of anthocyanins in seed testa of 5 peanut varieties(lines)

图2 5个ANS基因在5个花生品种(系)种皮中的表达分析

Fig.2 Expression analysis of 5 ANS genes in seed testa of 5 peanut varieties(lines)

图3 花生种皮中ANS基因的表达量与花青素的含量相关性分析 ns.相关性不显著;^*.在0.05水平上的相关性显著;^**.在0.01水平上的相关性显著;^***.0.001水平上的相关性显著。

Fig.3 Correlation analysis of ANS gene expression level and anthocyanin content in peanut seed testa ns.The correlation is not significant;^*.A significant correlation at the 0.05 level;^**.A significant correlation at the 0.01 level;^***.A significant correlation at the 0.001 level.

图4 候选基因结构

Fig.4 Candidate gene structure

表1 5个候选基因编码蛋白质的理化性质统计

Tab.1 Isoelectric points and molecular weight statistics of 5 candidate genes

基因 Gene	等电点 Isoelectric point	分子量/ku Molecular weight	亚细胞定位 Subcellular localization	亲/疏水性 Hydrophilic/hydrophobic
Ahy_Scaffold1g106620	5.60	39.929 89	细胞质	亲水
Ahy_A02g006729	6.05	42.190 12	细胞质	亲水
Ahy_B02g061206	5.53	42.637 53	细胞质	亲水
Ahy_B07g087995	5.75	32.739 29	细胞质	亲水
Ahy_B10g102589	6.41	41.000 69	细胞质	亲水

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