金花葵开花前后差异基因及代谢物分析

doi:10.7668/hbnxb.20193972

摘要/Abstract

摘要：

为深入研究金花葵花朵开花前后基因和代谢物的变化,利用转录组学和代谢组学技术相结合的方法对金花葵花蕾和花朵进行检测。结果显示,通过转录组分析鉴定了206 636个Unigenes,筛选出42 618个差异表达Unigenes,其中包括63个差异表达转录因子家族,24个转录调节因子家族。GO分析结果显示,差异表达基因主要富集于囊泡介导的逆行运输等生物学过程。KOG功能注释显示,差异表达基因功能以通用功能预测居多,其次是信号转导机制、翻译后修饰蛋白周转以及碳水化合物的转运和代谢。差异表达基因KEGG富集分析表明,差异基因主要富集于代谢、植物激素和信号转导、淀粉和蔗糖代谢等代谢通路。通过代谢组学检测,筛选到差异显著代谢物135个,主要包括脂类、氨基酸及衍生物和黄酮类等,KEGG富集分析表明,差异代谢物主要富集于甘油磷脂代谢、糖基磷脂酰肌醇(GPI)-锚定生物合成和次生代谢产物的生物合成-未分类等过程,其中,缬氨酸,亮氨酸和异亮氨酸的降解代谢通路同时出现在了基因和代谢物KEGG代谢通路富集Top 20中。

关键词: 金花葵, 开花, 基因, 代谢物

Abstract:

In order to study the changes of genes and metabolites pre- and post-flowering in Aurea helianthus,transcriptome and metabolome techniques were used to detect flower buds and flowers of Aurea helianthus. Through transcriptomic analysis,206 636 Unigenes were identified and 42 618 differentially expressed Unigenes were identified,including 63 differentially expressed transcription factor families and 24 transcription regulatory factor families. GO analysis showed that the differentially expressed genes were mainly enriched in biological processes such as retrograde vesicle-mediated transport. KOG functional annotation showed that the function of differentially expressed genes was mostly general function prediction only,followed by signal transduction mechanism,posttranslational modification,protein turnover,chaperones,and carbohydrate transport and metabolism. KEGG enrichment analysis showed that the differentially expressed genes were mainly enriched in metabolic pathways such as metabolism,plant hormone and signal transduction,starch and sucrose metabolism. A total of 135 metabolites were detected by metabolomics analysis,including lipid,flavonoids,amino acids and derivatives. KEGG enrichment analysis showed that differences metabolites mainly enriched in glycerophospholipid metabolism,glycosylphosphatidylinositol(GPI)-anchor biosynthesis and biosynthesis of secondary metabolites-unclassified,notably,valine,leucine and isoleucine degradation appeared simultaneously in the genes and metabolites KEGG enrichment Top 20 metabolic pathways.

Key words: Aurea helianthus, Flowering, Gene, Metabolite

甄军波, 宋世佳, 刘琳琳, 刘迪, 欧阳艳飞, 迟吉娜. 金花葵开花前后差异基因及代谢物分析[J]. 华北农学报, 2023, 38(S1): 58-66. doi: 10.7668/hbnxb.20193972.

ZHEN Junbo, SONG Shijia, LIU Linlin, LIU Di, OUYANG Yanfei, CHI Ji'na. Analysis of Differential Genes and Metabolites Pre- and Post-flowering in Aurea helianthus[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 58-66. doi: 10.7668/hbnxb.20193972.

http://www.hbnxb.net/CN/Y2023/V38/IS1/58

图/表 13

参考文献 26

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取样时期 Sampling period	样本名称 Sample name	分组 Group
花蕾Bud	L-1	AhB
花蕾Bud	L-2	AhB
花蕾Bud	L-3	AhB
开花当天Flowering day	H-1	AhF
开花当天Flowering day	H-2	AhF
开花当天Flowering day	H-3	AhF

取样时期 Sampling period	样本名称 Sample name	分组 Group
花蕾Bud	L-1	AhB
花蕾Bud	L-2	AhB
花蕾Bud	L-3	AhB
开花当天Flowering day	H-1	AhF
开花当天Flowering day	H-2	AhF
开花当天Flowering day	H-3	AhF

引物名称 Primer	引物序列(5'—3') Primer sequence(5'—3')	引物用途 Purpose
92208F	CCTTGTCGGGCAAAGTAAACC	荧光定量
92208R	CGGAGGAGCAACTCAGTCAGC	荧光定量
115186F	CGGGAGGTTTCGGGTAATGAT	荧光定量
115186R	CAGGCTTTCCAGAACTGGGTT	荧光定量
92019F	TTGCTCCGAATAGCCTACCG	荧光定量
92019R	CGACAAACACTCCGTCCACC	荧光定量
97829F	TTGGATGATTGGGTTCTTTGC	荧光定量
97829R	CGGGTCCGTTACACTGGTTAT	荧光定量
140405F	AGGAGTATTGGTTCCGTTGGTC	荧光定量
140405R	CATCTTGTCTTGGAGGAGTTGC	荧光定量
87703F	ACGAGTCGGCTAAGATTGTTTC	荧光定量
87703R	CAAGAACTCAACCTGACGCTGT	荧光定量
100232F	TTTCCAGCTTCGTCCGTCAG	荧光定量
100232R	GGCGATGTTGTCACCGTCTT	荧光定量
78465F	GCATGTCTCGGTCGGATGT	荧光定量
78465R	TGGCACGCCTCTTTCAAGT	荧光定量
H2A-1F	TATCTTGCCGCCGAGGTTCT	内参引物
H2A-1R	GAGGAGGTTGTGGATGTTGG	内参引物

引物名称 Primer	引物序列(5'—3') Primer sequence(5'—3')	引物用途 Purpose
92208F	CCTTGTCGGGCAAAGTAAACC	荧光定量
92208R	CGGAGGAGCAACTCAGTCAGC	荧光定量
115186F	CGGGAGGTTTCGGGTAATGAT	荧光定量
115186R	CAGGCTTTCCAGAACTGGGTT	荧光定量
92019F	TTGCTCCGAATAGCCTACCG	荧光定量
92019R	CGACAAACACTCCGTCCACC	荧光定量
97829F	TTGGATGATTGGGTTCTTTGC	荧光定量
97829R	CGGGTCCGTTACACTGGTTAT	荧光定量
140405F	AGGAGTATTGGTTCCGTTGGTC	荧光定量
140405R	CATCTTGTCTTGGAGGAGTTGC	荧光定量
87703F	ACGAGTCGGCTAAGATTGTTTC	荧光定量
87703R	CAAGAACTCAACCTGACGCTGT	荧光定量
100232F	TTTCCAGCTTCGTCCGTCAG	荧光定量
100232R	GGCGATGTTGTCACCGTCTT	荧光定量
78465F	GCATGTCTCGGTCGGATGT	荧光定量
78465R	TGGCACGCCTCTTTCAAGT	荧光定量
H2A-1F	TATCTTGCCGCCGAGGTTCT	内参引物
H2A-1R	GAGGAGGTTGTGGATGTTGG	内参引物

样本 Sample	原始数据 Raw reads	干净序列 Clean reads	错误率/% Error rate	Q30/%	GC含量/% GC content
L-1	73 503 118	71 644 532	0.02	93.56	44.54
L-2	59 489 452	58 093 036	0.02	92.99	44.62
L-3	55 725 072	54 385 274	0.02	93.33	44.46
H-1	59 386 114	58 147 996	0.02	93.38	44.20
H-2	60 959 762	59 618 350	0.02	93.63	44.16
H-3	49 665 928	48 539 808	0.02	93.99	44.26

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