华北农学报 ›› 2025, Vol. 40 ›› Issue (3): 207-214. doi: 10.7668/hbnxb.20194898

所属专题: 畜牧

• 畜牧·水产·兽医 • 上一篇    下一篇

基于肝脏转录组测序筛选康乐黄鸡开产相关基因和关键通路

马荆鄂, 曾配君, 万淑敏, 熊信威, 王樟凤, 刘婧, 许继国, 饶友生   

  1. 南昌师范学院 生物技术研究院,家禽遗传改良江西省重点实验室,江西 南昌 330032
  • 收稿日期:2024-10-26 出版日期:2025-07-04
  • 通讯作者:
    饶友生(1965—),男,江西永丰人,教授,博士,主要从事家禽遗传育种与繁殖研究。
  • 作者简介:

    马荆鄂(1987—),女,湖北荆州人,副教授,博士,主要从事家禽遗传育种与繁殖研究。

  • 基金资助:
    国家自然科学基金项目(32160784); 江西省高等学校教学改革研究重点课题项目(JXJG-23-23-5); 大学生创新创业训练计划项目(202314437002)

Screening of Genes and Key Pathways Related to Egg-Laying in Kangle Yellow Chickens Based on Liver Transcriptome Sequencing

MA Jinge, ZENG Peijun, WAN Shumin, XIONG Xinwei, WANG Zhangfeng, LIU Jing, XU Jiguo, RAO Yousheng   

  1. Institution of Biological Technology,Nanchang Normal University,Key Laboratory of Poultry Genetic Improvement of Jiangxi Province,Nanchang 330032,China
  • Received:2024-10-26 Published:2025-07-04

摘要:

通过分析开产与未开产康乐黄鸡肝脏组织转录组表达谱,筛选出开产相关候选基因和关键通路,为研究鸡肝脏基因调控开产性状分子机理提供理论基础,为康乐黄鸡选种选育提供一定参考。选取154日龄已开产(H组)、未开产(L组)各3个个体的肝脏组织,采用TRIzol法提取总RNA,利用Illumina测序平台进行转录组测序,筛选2组个体的差异表达基因;对差异表达基因进行功能富集和蛋白互作分析;随机选取9个候选基因,在9个个体肝脏中,进行qRT-PCR验证。一共检测到21 465个基因在肝脏组织表达,共筛选出227个差异表达基因,其中48个表达上调,179个表达下调。qRT-PCR验证结果表明,9个基因在2组个体肝脏中表达量变化趋势与RNA-Seq结果基本一致,且在H、M(即将开产)和L 3组个体中呈现表达量依次递减或递增的趋势。初步确定了6个开产性状相关候选基因,分别是VTG1VTG2VTG3APOV1RBPRNF186。5条关键信号通路分别是脂肪消化吸收、胆固醇代谢、ECM-受体相互作用、雌激素信号通路、D-谷氨酰胺和D-谷氨酸代谢。综上,初步确定了6个康乐黄鸡开产性状相关候选基因,5条关键信号通路。

关键词: 康乐黄鸡, 肝脏, 候选基因, 关键通路, 转录组测序, 开产性状

Abstract:

The purpose of this study was to screen the candidate genes and key pathways related to traits at first laying through transcriptomic analysis of liver tissues on laying and non-laying Kangle yellow chickens,and to provide a theoretical basis for studying the molecular mechanisms of laying traits regulation in the liver of chickens.It also provides certain reference for the selection and breeding of Kangle yellow chickens.Three individuals of liver tissues at 154 days of age that had started laying (Group H) and had not started laying (Group L) were selected.Total RNA was extracted using the TRIzol method,and transcriptome sequencing was performed using the Illumina sequencing platform.Differentially expressed genes between the two groups were identified.The differentially expressed genes were subjected to functional enrichment and protein-protein interaction analysis.Nine candidate genes were randomly selected,and their expression levels were verified by qRT-PCR in the livers of the nine individuals.A total of 21 465 genes were detected to be expressed in the liver tissues,and a total of 227 differentially expressed genes were identified,among which 48 were up-regulated and 179 were down-regulated.The qRT-PCR validation results showed that the expression trends of nine genes in the two groups of individuals were basically consistent with the RNA-Seq results,and they showed a decreasing or increasing trend in the H,M(to be laid),and L three groups.Six candidate genes for the age at egg-laying trait were initially identified,namely VTG1,VTG2,VTG3,APOV1,RBP,and RNF186.The five crucial signaling pathways were fat digestion and absorption,cholesterol metabolism,ECM-receptor interaction,estrogen signaling pathway,D-glutamine and D-glutamate metabolism.Six genes and five key signaling pathways were preliminarily identified related to the traits at the first laying of Kangle yellow chicken.

Key words: Kangle yellow chicken, Liver, Candidate gene, Key pathway, Transcriptome sequencing, Egg-laying trait

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引用本文

马荆鄂, 曾配君, 万淑敏, 熊信威, 王樟凤, 刘婧, 许继国, 饶友生. 基于肝脏转录组测序筛选康乐黄鸡开产相关基因和关键通路[J]. 华北农学报, 2025, 40(3): 207-214. doi: 10.7668/hbnxb.20194898.

MA Jinge, ZENG Peijun, WAN Shumin, XIONG Xinwei, WANG Zhangfeng, LIU Jing, XU Jiguo, RAO Yousheng. Screening of Genes and Key Pathways Related to Egg-Laying in Kangle Yellow Chickens Based on Liver Transcriptome Sequencing[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 207-214. doi: 10.7668/hbnxb.20194898.