小麦科大116&times;科大101 F<sub>2</sub>群体穗长主效QTL定位及候选基因预测

doi:10.7668/hbnxb.20194275

摘要/Abstract

摘要：

穗长是小麦重要的农艺性状,与产量构成因素密切相关。旨在研究小麦穗长遗传基因,筛选与穗长基因连锁的分子标记,为小麦分子标记辅助育种提供分子支撑。以科大116和科大101为亲本构建的F₂群体为试验材料,通过SSR分子标记,构建覆盖小麦基因组的遗传图谱,并结合完备复合区间作图法对穗长进行QTL定位。采用3 234对引物,共筛选出434对在双亲间具有多态性的引物,多态性引物的检出率为13.42%。通过BSA混池分析,共筛选出28个可能与穗长连锁的分子标记,其中16个标记通过262株群体验证与目标基因紧密连锁。通过QTL-IciMapping软件构建小麦染色体组的遗传图谱,标记间的平均遗传距离为38.66 cM,共检测出7个与穗长相关的QTL位点,分别位于3B、4A、4B和6B染色体上,其加性效应值均为正值,对表型性状遗传变异的贡献率在4.01%~23.16%。在4B染色体上定位出2个主效QTL位点,贡献率为17.59%~23.16%。其中,Qsl4B-2距离其最近的分子标记只有3.5 cM,为连锁最紧密的一个QTL位点,分析发现其可能为新的主效QTL位点。因此,4B染色体上可能存在与穗长相关的基因。通过分析预测,在4B染色体的yzu397456~yzu404917和yzu409422~yzu405167标记区间内,可能存在7个调控小麦穗长的候选基因,在穗中持续高表达。

关键词: 小麦, F₂群体, 穗长, QTL, 候选基因

Abstract:

Spike length is an important agronomic trait in wheat and is closely related to yield components.Studying wheat spike length genes and screening molecular markers linked to spike length genes can provide molecular support for wheat molecular marker-assisted breeding.The F₂ population constructed with Keda 116 and Keda 101 as parents was used as test material to construct a genetic map covering the wheat genome by SSR molecular markers and to locate QTLs for spike length by combining the complete composite interval mapping method.A total of 434 pairs of primers with polymorphism between parents were screened from 3 234 pairs of primers,and the detection rate of polymorphic primers was 13.42%.A total of 28 molecular markers were screened for possible linkage to spike length by BSA mixed pool analysis,16 of these markers were verified to be tightly linked to the target gene by a population of 262 plants.The genetic map of wheat chromosome group was constructed by QTL-IciMapping software,and the average genetic distance between markers was 38.66 cM.A total of seven QTLs loci associated with spike length were detected,which were located on chromosomes 3B,4A,4B and 6B.The additive effect values of all the seven QTLs were positive and their contributions to the genetic variation of phenotypic traits ranged from 4.01% to 23.16%.Two major QTLs were mapped on chromosome 4B,explaining 17.59%-23.16% of the phenotypic variance.Among them,Qsl4B-2 was the most closely linked QTL locus with only 3.5 cM away from the nearest molecular marker,and the analysis found that it might be a new major QTL locus.Therefore,genes associated with spike length might exist on chromosome 4B.Within the marker interval of chromosome 4B from yzu397456 to yzu404917 and yzu409422 to yzu405167,there might be seven candidate genes regulating wheat spike length,which were consistently highly expressed in the spike.

Key words: Wheat, F₂ population, Spike length, QTL, Candidate gene

郭媛, 马指挥, 王世雨, 牛志鹏, 杨晓雨, 魏青, 陈安琪, 王林生. 小麦科大116×科大101 F₂群体穗长主效QTL定位及候选基因预测[J]. 华北农学报, 2023, 38(5): 84-93. doi: 10.7668/hbnxb.20194275.

GUO Yuan, MA Zhihui, WANG Shiyu, NIU Zhipeng, YANG Xiaoyu, WEI Qing, CHEN Anqi, WANG Linsheng. Major QTL Mapping and Candidate Gene Prediction of Spike Length in F₂Population of Wheat Keda 116 × Keda 101[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(5): 84-93. doi: 10.7668/hbnxb.20194275.

http://www.hbnxb.net/CN/Y2023/V38/I5/84

图/表 7

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性状 Trait	亲本 Parent		F₂群体 F₂ population
性状 Trait	科大116 Keda 116	科大101 Keda 101	平均值 Mean	最大值 Maximum	最小值 Minimum	标准差 s	偏度 Skewness	峰度 Kurtosis	变异系数/% CV
穗长/cm Spike length	9.70	19.80	13.01	18.20	8.90	1.54	-0.02	0.55	11.84

性状 Trait	亲本 Parent		F₂群体 F₂ population
性状 Trait	科大116 Keda 116	科大101 Keda 101	平均值 Mean	最大值 Maximum	最小值 Minimum	标准差 s	偏度 Skewness	峰度 Kurtosis	变异系数/% CV
穗长/cm Spike length	9.70	19.80	13.01	18.20	8.90	1.54	-0.02	0.55	11.84

QTL	染色体 Chromosome	位置/cM Position	标记区间 Marker interval	LOD	表型贡献率/% PVE	加性效应 Additive effect
Qsl3B-1	3B	5	yzu273497~yzu294190	6.60	13.11	0.35
Qsl4A-1	4A	45	yzu328892~yzu341540	10.07	12.90	1.04
Qsl4A-2	4A	76	yzu341540~yzu330490	6.71	12.70	1.20
Qsl4B-1	4B	103	yzu397456~yzu404917	9.43	17.59	0.86
Qsl4B-2	4B	296	yzu409422~yzu405167	12.60	23.16	0.86
Qsl6B-1	6B	31	yzu588540~yzu583898	3.02	4.01	0.82
Qsl6B-2	6B	125	yzu583898~yzu610944	6.07	10.81	1.38

QTL	染色体 Chromosome	位置/cM Position	标记区间 Marker interval	LOD	表型贡献率/% PVE	加性效应 Additive effect
Qsl3B-1	3B	5	yzu273497~yzu294190	6.60	13.11	0.35
Qsl4A-1	4A	45	yzu328892~yzu341540	10.07	12.90	1.04
Qsl4A-2	4A	76	yzu341540~yzu330490	6.71	12.70	1.20
Qsl4B-1	4B	103	yzu397456~yzu404917	9.43	17.59	0.86
Qsl4B-2	4B	296	yzu409422~yzu405167	12.60	23.16	0.86
Qsl6B-1	6B	31	yzu588540~yzu583898	3.02	4.01	0.82
Qsl6B-2	6B	125	yzu583898~yzu610944	6.07	10.81	1.38

基因序列号 Gene ID	注释信息 Annotation information	物理位置/bp Physical position
TraesCS4B03G0641900	四磷酸肌醇蛋白	490 160 484-490 166 575
TraesCS4B03G0643200	角鲨烯单加氧酶重组蛋白	490 974 724-490 979 705
TraesCS4B03G0644300	GTP结合蛋白	492 900 179-492 920 025
TraesCS4B03G0645200	卵磷脂-胆固醇酰基转移酶样蛋白	493 355 473-493 357 077

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小麦科大116×科大101 F₂群体穗长主效QTL定位及候选基因预测

Major QTL Mapping and Candidate Gene Prediction of Spike Length in F₂Population of Wheat Keda 116 × Keda 101

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基因序列号 Gene ID	注释信息 Annotation information	物理位置/bp Physical position
TraesCS4B02G238800	COG6蛋白	494 726 389-494 732 247
TraesCS4B02G238900	核孔复合蛋白NUP133	494 732 486-494 739 166
TraesCS4B02G239000	ERD15蛋白	495 376 183-495 377 420

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小麦科大116×科大101 F2群体穗长主效QTL定位及候选基因预测

Major QTL Mapping and Candidate Gene Prediction of Spike Length in F2 Population of Wheat Keda 116 × Keda 101

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小麦科大116×科大101 F₂群体穗长主效QTL定位及候选基因预测

Major QTL Mapping and Candidate Gene Prediction of Spike Length in F₂Population of Wheat Keda 116 × Keda 101