华北农学报 ›› 2025, Vol. 40 ›› Issue (3): 44-50. doi: 10.7668/hbnxb.20195535

所属专题: 生物技术 蔬菜专题

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

基于极端混合池全基因组重测序的茄子果实硬度基因定位

乔军1,2,3, 刘婧2,3,4, 李素文1,2,3, 王利英1,2,3,   

  1. 1 天津市农业科学院 蔬菜研究所,天津 300381
    2 蔬菜生物技术育种国家重点实验室,天津 300381
    3 天津市蔬菜遗传育种企业重点实验室,天津 300381
    4 天津科润蔬菜研究所,天津 300381
  • 收稿日期:2024-10-28 出版日期:2025-07-04
  • 通讯作者:
    王利英(1971―),女,天津人,研究员,硕士,主要从事茄子育种研究。
  • 作者简介:

    乔 军(1986―),男,山西浑源人,副研究员,硕士,主要从事茄子育种研究。

  • 基金资助:
    蔬菜生物技术育种国家重点实验室项目(24ZXZSSS00210); 天津市蔬菜现代农业产业技术体系创新团队项目(ITTVRS2024007)

Prediction of Fruit Hardness Genes of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool

QIAO Jun1,2,3, LIU Jing2,3,4, LI Suwen1,2,3, WANG Liying1,2,3,   

  1. 1 Tianjin Vegetable Research Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China
    2 State Key Laboratory of Vegetable Germplasm Innovation,Tianjin 300381,China
    3 Tianjin Enterprise Key Laboratory of Vegetable Genetics & Breeding,Tianjin 300381,China
    4 Tianjin Kernel Vegetable Research Institute,Tianjin 300381,China
  • Received:2024-10-28 Published:2025-07-04

摘要:

为系统研究果实硬度相关基因,通过基因组重测序BSA方法定位果实硬度关联区间,并根据其对应的参考基因组共线性区段和基因注释信息预测候选基因,为下一步基因克隆奠定基础。以稳定遗传的软肉自交系C18与硬肉自交系LE4杂交,F2后代的果实硬度分离呈正态分布。在F2群体中分别选取30株软肉和30株硬肉单株构建极端混合池,对混合池样本和双亲材料分别开展30×和10×覆盖度的全基因组重测序。重测序共获得1 891 040个单核苷酸多态性(SNPs)标记及376 603个插入缺失(InDels)标记,用于果实硬度性状的全基因组定位。BSA定位峰值位于茄子第6号染色体,从72 610 411到75 329 951 bp,共计2.71 Mb。根据通路富集和基因功能注释,筛选到候选基因Smechr0601726.1Smechr0601735.1。综上,通过基因组重测序BSA分析可知,茄子果实硬度可能由2个重要的候选基因进行调控,Smechr0601726.1编码多聚半乳糖醛酸酶基因,与果实硬度直接相关;Smechr0601735.1与抗坏血酸和阿糖二酸代谢密切相关,编码抗坏血酸过氧化物酶,与果实发育和硬度形成有关,可以延缓果实软化。

关键词: 茄子, 极端混合池, 全基因组重测序, 果实硬度, 基因定位

Abstract:

To systematically study genes related to fruit hardness,genome resequencing BSA method was used to locate the fruit hardness association interval,and predict candidate genes based on their corresponding reference genome collinearity segments and gene annotation information,to lay the foundation for the next step of gene localization and cloning.The fruit hardness separation of F2 offspring from stable genetic soft flesh inbred line C18 and hard flesh inbred line LE4 crosses followed a normal distribution.30 soft fleshed and 30 hard fleshed individual plants were selected from the F2 population to construct extreme mixed pools,and whole genome resequencing with 30×and 10×coverage on the mixed pools and parents was conducted.A total of 1 891 040 single nucleotide polymorphisms(SNPs)and 376 603 insertion deletion markers(InDels)were obtained from the hybrid pools and parents,which were used for genome-wide mapping of fruit hardness traits.The peak of BSA localization was distributed within a total of 2.71 Mb between 72 610 411 and 75 329 951 bp on eggplant chromosome 6.Based on pathway enrichment and gene function annotation,candidate genes Smechr0601726.1 and Smechr0601735.1 were obtained.In summary,through genome resequencing BSA analysis,eggplant fruit hardness may be regulated by two important candidate genes.Smechr0601726.1 encodes a polygalacturonase gene,which is directly related to fruit hardness;Smechr0601735.1 is closely related to the metabolism of ascorbic acid and arabic acid,encoding ascorbate peroxidase,and is associated with fruit development and hardness formation,which can delay fruit softening.

Key words: Eggplant, Extreme mixing pool, Whole-genome resequencing, Fruit hardness, Gene mapping

中图分类号: 

引用本文

乔军, 刘婧, 李素文, 王利英. 基于极端混合池全基因组重测序的茄子果实硬度基因定位[J]. 华北农学报, 2025, 40(3): 44-50. doi: 10.7668/hbnxb.20195535.

QIAO Jun, LIU Jing, LI Suwen, WANG Liying. Prediction of Fruit Hardness Genes of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 44-50. doi: 10.7668/hbnxb.20195535.