利用籼粳交RIL群体进行水稻发芽期与芽期耐冷性QTL分析

doi:10.7668/hbnxb.20192749

摘要/Abstract

摘要：

为定位控制水稻发芽期和芽期耐冷性的QTL,探究其遗传机制,以耐冷性强的粳稻品种彩稻为母本、耐冷性弱的籼稻品种WD为父本杂交衍生的含有189个株系的重组自交系群体为试验材料,以低温胁迫下的相对发芽率、平均发芽天数、成苗率、芽期耐冷级别为鉴定指标,结合978个Bin标记,运用IciMapping 4.2对发芽期和芽期耐冷QTL进行定位分析。结果显示,检测到2对与芽期耐冷有关的上位性QTL,LOD值分别为5.70,5.55,贡献率分别为13.22%,39.87%;此外,还检测到5个与水稻发芽期耐冷相关的QTL、5个与水稻芽期耐冷相关的QTL。这些QTL位于1,3,5,8号染色体上,LOD值为2.55~7.07,表型贡献率为6.41%~17.17%。其中,qLTG3在5个发芽期耐冷性QTL中表型贡献率最高,且与芽期耐冷性相关;qCTP1-2在5个芽期耐冷性QTL中表型贡献率最高;qCTB1、qCTP1-2和qLTG8等3个QTL未见报道。研究检测到的QTL以及上位性QTL,可为后续水稻耐冷性的遗传研究以及改良提供参考。

关键词: 水稻, 发芽期, 芽期, 耐冷性, 数量性状位点

Abstract:

To map QTLs that control cold tolerance at germination and bud stages,to explore the genetic mechanism,a recombinant inbred line(RIL)population was used as experimental material.The RIL population contained 189 lines derived from the cross between Caidao,a japonica rice variety that had strong cold tolerance and was adopted as the female parent,and WD,a indica rice variety that had weak cold tolerance and was used as the male parent.The QTLs that control cold tolerance at germination and bud stage were mapped between four phenotypic indexes and 978 Bin markers by IciMapping 4.2.The four phenotypic indexes included relative germination rate,average germination days,seedling rate and cold tolerance level.The results showed that two pairs of epistatic QTLs related to cold tolerance at bud stage were mapped.The LOD scores were 5.70 and 5.55 and the phenotypic contribution rates were 13.22% and 39.87%,respectively.In addition,five and five QTLs that were related to cold tolerance at germination and bud stage were mapped,respectively.These QTLs were located on chromosomes 1,3,5 and 8.The LOD score and phenotypic contribution rate of these QTLs ranged from 2.55 to 7.07 and from 6.41% to 17.17%,respectively.qLTG3 had the highest phenotypic contribution rate among the five germination stage QTLs and was also related to cold tolerance at germination stage.qCTP1-2 had the maximum of phenotypic contribution rate among the five bud stage QTLs.qCTB1,qCTP1-2 and qLTG8 had not been reported.The QTLs and epistatic QTLs detected will provide a reference for the genetic research and improvement of cold tolerance in rice.

Key words: Rice, Germination stage, Bud stage, Cold tolerance, QTL

陈子琪, 王伟苹, 赵宏强, 王皓, 韩笑, 杨洛淼, 辛威, 刘化龙, 郑洪亮, 王敬国. 利用籼粳交RIL群体进行水稻发芽期与芽期耐冷性QTL分析[J]. 华北农学报, 2022, 37(1): 50-57. doi: 10.7668/hbnxb.20192749.

CHEN Ziqi, WANG Weiping, ZHAO Hongqiang, WANG Hao, HAN Xiao, YANG Luomiao, XIN Wei, LIU Hualong, ZHENG Hongliang, WANG Jingguo. QTL Analysis of Rice Cold Tolerance at Germination and Bud Stage Utilizing Indica-Japonica RIL Population[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 50-57. doi: 10.7668/hbnxb.20192749.

http://www.hbnxb.net/CN/Y2022/V37/I1/50

图/表 7

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级别 Level	秧苗恢复状态 Seedling recovery state
0	恢复后幼芽死亡
1	恢复后幼芽尚未死亡,但发育停止
2	恢复后发育非常缓慢,叶色淡
3	恢复后发育较慢,叶色较淡
4	恢复后能够正常生长,叶色较深

级别 Level	秧苗恢复状态 Seedling recovery state
0	恢复后幼芽死亡
1	恢复后幼芽尚未死亡,但发育停止
2	恢复后发育非常缓慢,叶色淡
3	恢复后发育较慢,叶色较淡
4	恢复后能够正常生长,叶色较深

染色体 Chromosome	染色体长度 /cM Chromosome length	标记数/个 Number of marker	标记间平均距离 /cM Mean distance between adjacent Bins
1	308.99	120	2.57
2	269.94	86	3.14
3	245.66	57	4.31
4	201.55	98	2.06
5	164.41	84	1.96
6	127.90	60	2.13
7	183.46	54	3.40
8	218.24	90	2.42
9	159.53	49	3.26
10	163.25	74	2.21
11	229.56	140	1.64
12	192.84	66	2.92

染色体 Chromosome	染色体长度 /cM Chromosome length	标记数/个 Number of marker	标记间平均距离 /cM Mean distance between adjacent Bins
1	308.99	120	2.57
2	269.94	86	3.14
3	245.66	57	4.31
4	201.55	98	2.06
5	164.41	84	1.96
6	127.90	60	2.13
7	183.46	54	3.40
8	218.24	90	2.42
9	159.53	49	3.26
10	163.25	74	2.21
11	229.56	140	1.64
12	192.84	66	2.92

时期 Period	性状 Trait	亲本Parents		重组自交系RIL population
时期 Period	性状 Trait	彩稻Caidao	WD	平均值±标准差 Means±s	变幅 Range	变异系数/% CV	偏度 Kurtosis	峰度 Skewness
发芽期	相对发芽率/%	90.82±1.29a	41.60±4.39b	59.16±14.07	3.49~95.45	23.78	-0.36	-0.93
Germination stage	平均发芽天数/d	11.21±0.47b	14.61±0.30a	13.21±3.59	8.00~15.67	27.18	-0.99	1.46
芽期	成苗率/%	75.00±2.55a	40.00±4.11b	68.44±22.60	10.00~100.00	33.02	-0.84	-0.16
Bud stage	耐冷级别	2.25±0.17a	1.00±0.09b	2.08±0.68	0.45~3.39	32.69	-0.45	-0.63

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