水稻<i>DEP1</i>突变体籽粒品质性状及其代谢组分析

doi:10.7668/hbnxb.20194099

摘要/Abstract

摘要：

为分析DEP1等位变异对水稻籽粒品质和籽粒代谢轮廓的影响。以优质粳稻品种稻花香及其直立穗型突变体dep1-1为试材,测定了籽粒的品质性状及差异代谢产物。结果表明,突变体dep1-1在DEP1基因第5外显子处存在1个单碱基A插入,导致翻译提前终止,从而使G蛋白γ亚基富含半胱氨酸的区域缺失。与野生型品种相比,突变体dep1-1的粒长、外观、黏度、平衡度、食味值、峰值、回复值和峰值时间等指标显著降低,而直链淀粉含量、酸度值和硬度显著提高。与野生型相比,突变体dep1-1中37个代谢物含量显著上调,而45个代谢物含量显著下调。KEGG分析表明,差异代谢物主要富集于半乳糖代谢、脂肪酸生物合成、淀粉蔗糖代谢、亚油酸代谢、ABC转运蛋白、类苯基丙烷生物合成等途径。其中,与野生型品种相比,突变体dep1-1籽粒的蔗糖、D-棉子糖、棉子糖、D-(+)-麦芽糖D-(+)、甜菜碱、吲哚含量显著提高,而硬脂酸、软脂酸、L-亮氨酸、吲哚乙酸含量显著下降。上述的差异代谢物初步解释了突变体dep1-1籽粒品质变化的生理生化机制。

关键词: 水稻, 直立穗型, DEP1, 代谢组, 籽粒品质

Abstract:

In order to determine the effect of allele variation in DEP1 locus on quality traits and metabolic profile of grains in rice,the high quality japonica cultivar Daohuaxiang and its erect panicle type mutant dep1-1 were used.The grain quality traits and differential metabolites between wild-type and mutant dep1-1 were measured.The results showed that the mutant dep1-1 had a single base A inserted into the fifth exon of the DEP1 gene,leading to premature termination of translation and the deletion of the cysteine-rich region in G-protein gamma subunit.Compared with wild-type cultivar,the grain length,appearance,viscosity,balance,taste value,peak value,recovery value and peak time of mutant dep1-1 were significantly decreased,while amylose content, acidity value and hardness were significantly increased.Compared with wild-type cultivar,37 metabolites were significantly up-regulated in dep1-1,while 45 were significantly down-regulated.KEGG analysis showed that these differential metabolites were mainly enriched in galactose metabolism,fatty acid biosynthesis,starch sucrose metabolism,linoleic acid metabolism,ABC transporters,and phenylpropanoid biosynthesis pathways.Compared with wild-type cultivar,the contents of sucrose,D-raffinose,raffinose,D-(+)-maltose D-(+),betaine and indole of grains significantly increased in dep1-1,while those of stearic acid,palmitic acid,L-leucine and indole-3-acetic acid significantly decreased.These differential metabolites preliminarily explained the physiological and biochemical mechanism of grain quality changes in dep1-1 mutant.

Key words: Rice, Erect panicle type, DEP1, Metabolome, Grain quality

赵明珠, 王丽丽, 马作斌, 唐志强, 郑文静. 水稻DEP1突变体籽粒品质性状及其代谢组分析[J]. 华北农学报, 2023, 38(S1): 1-7. doi: 10.7668/hbnxb.20194099.

ZHAO Mingzhu, WANG Lili, MA Zuobin, TANG Zhiqiang, ZHENG Wenjing. Grain Quality Characters and Metabolome Analysis of DEP1 Mutant in Rice[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 1-7. doi: 10.7668/hbnxb.20194099.

http://www.hbnxb.net/CN/Y2023/V38/IS1/1

图/表 5

参考文献 21

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性状Trait		野生型Wild type	突变体dep1-1
粒形	粒长/mm	8.55±0.96Aa	7.52±1.03Ab
Seed shape	粒宽/mm	2.79±0.87Aa	2.84±0.67Aa
	粒厚/mm	2.15±0.59Aa	2.09±0.43Aa
化学品质	蛋白质含量/%	7.30±0.89Aa	7.73±1.21Aa
Chemical quality	直链淀粉含量/%	14.50±0.64Ab	15.53±0.65Aa
食味品质	酸度值	23.05±0.68Ab	24.33±0.74Aa
Eating quality	外观	4.57±0.55Aa	3.52±0.61Ab
	硬度	7.45±0.19Ab	7.84±0.11Aa
	黏度	5.23±1.14Aa	3.81±1.01Bb
	平衡度	4.73±1.01Aa	3.49±0.98Ab
	食味值	58.33±2.56Aa	50.94±2.08Bb
RVA性状	峰值	2 750.50±368.35Aa	1 817.00±298.95Bb
RVA traits	崩解值/cP	837.50±105.25Aa	704.00±98.80Ab
	黏度/cP	2 950.00±995.52Aa	1 776.33±867.69Bb
	回复值/cP	1 037.00±285.88Aa	763.33±237.85Ab
	峰值时间/min	6.40±0.91Aa	5.36±0.84Ab
	糊化温度/℃	69.40±0.53Aa	69.68±0.64Aa

性状Trait		野生型Wild type	突变体dep1-1
粒形	粒长/mm	8.55±0.96Aa	7.52±1.03Ab
Seed shape	粒宽/mm	2.79±0.87Aa	2.84±0.67Aa
	粒厚/mm	2.15±0.59Aa	2.09±0.43Aa
化学品质	蛋白质含量/%	7.30±0.89Aa	7.73±1.21Aa
Chemical quality	直链淀粉含量/%	14.50±0.64Ab	15.53±0.65Aa
食味品质	酸度值	23.05±0.68Ab	24.33±0.74Aa
Eating quality	外观	4.57±0.55Aa	3.52±0.61Ab
	硬度	7.45±0.19Ab	7.84±0.11Aa
	黏度	5.23±1.14Aa	3.81±1.01Bb
	平衡度	4.73±1.01Aa	3.49±0.98Ab
	食味值	58.33±2.56Aa	50.94±2.08Bb
RVA性状	峰值	2 750.50±368.35Aa	1 817.00±298.95Bb
RVA traits	崩解值/cP	837.50±105.25Aa	704.00±98.80Ab
	黏度/cP	2 950.00±995.52Aa	1 776.33±867.69Bb
	回复值/cP	1 037.00±285.88Aa	763.33±237.85Ab
	峰值时间/min	6.40±0.91Aa	5.36±0.84Ab
	糊化温度/℃	69.40±0.53Aa	69.68±0.64Aa

代谢物编号 Metabolite number	代谢物差异 log₂FC	物质名称 Chemical name	化合物分子式 Compound formula	代谢通路 KEGG
Com_16_neg	0.18	蔗糖	C₁₂ H₂₂ O₁₁	ABC转运蛋白;半乳糖代谢;淀粉蔗糖代谢
Com_34_neg	0.39	D-棉子糖	C₁₈ H₃₂ O₁₆	ABC转运蛋白;半乳糖代谢
Com_17_neg	-0.23	硬脂酸	C₁₈ H₃₆ O₂	不饱和脂肪酸生物合成;脂肪酸生物合成
Com_79_neg	0.43	棉子糖	C₁₈ H₃₂ O₁₆	ABC转运蛋白;半乳糖代谢
Com_97_neg	0.49	胞啶	C₉ H₁₃ N₃ O₅	嘧啶代谢
Com_45_pos	0.46	D-(+)-麦芽糖D-(+)	C₁₂ H₂₂ O₁₁	ABC转运蛋白;淀粉蔗糖代谢
Com_68_pos	0.45	甜菜碱	C₅ H₁₁ NO₂	ABC转运蛋白;甘氨酸、丝氨酸和苏氨酸代谢
Com_40_pos	0.13	L-焦谷氨酸	C₅ H₇ NO₃	谷胱甘肽代谢
Com_103_pos	-0.40	9-Oxo-ODE	C₁₈ H₃₀ O₃	亚油酸代谢
Com_144_pos	-0.51	13-HPODE	C₁₈ H₃₂ O₄	亚油酸代谢
Com_162_pos	-0.32	2-羟基肉桂酸	C₉ H₈ O₃	次级代谢生物合成;类苯基丙烷生物合成;苯丙氨酸代谢
Com_351_pos	0.51	吲哚	C₈ H₇ N	次级代谢生物合成;苯丙氨酸、酪氨酸和色氨酸生物合成;色氨酸代谢
Com_397_pos	0.59	酪氨酸	C₉ H₁₁ NO₃	次级代谢生物合成;氨基酸生物合成;类苯基丙烷生物合成;苯丙氨酸、酪氨酸和色氨酸生物合成
Com_292_pos	0.50	阿魏酸	C₁₀ H₁₀ O₄	次级代谢生物合成;类苯基丙烷生物合成
Com_421_pos	-0.54	吲哚乙酸	C₁₀ H₉ NO₂	色氨酸代谢;植物激素信号传导
Com_209_pos	-0.26	亮氨酸	C₆ H₁₃ NO₂	次级代谢生物合成;氨基酸生物合成;ABC转运蛋白;2-氧羧酸代谢;氨酰生物合成;缬氨酸、亮氨酸和异亮氨酸的生物合成;缬氨酸、亮氨酸和异亮氨酸的降解
Com_249_pos	-0.21	软脂酸	C₁₆ H₃₀ O₂	脂肪酸生物合成

代谢物编号 Metabolite number	代谢物差异 log₂FC	物质名称 Chemical name	化合物分子式 Compound formula	代谢通路 KEGG
Com_16_neg	0.18	蔗糖	C₁₂ H₂₂ O₁₁	ABC转运蛋白;半乳糖代谢;淀粉蔗糖代谢
Com_34_neg	0.39	D-棉子糖	C₁₈ H₃₂ O₁₆	ABC转运蛋白;半乳糖代谢
Com_17_neg	-0.23	硬脂酸	C₁₈ H₃₆ O₂	不饱和脂肪酸生物合成;脂肪酸生物合成
Com_79_neg	0.43	棉子糖	C₁₈ H₃₂ O₁₆	ABC转运蛋白;半乳糖代谢
Com_97_neg	0.49	胞啶	C₉ H₁₃ N₃ O₅	嘧啶代谢
Com_45_pos	0.46	D-(+)-麦芽糖D-(+)	C₁₂ H₂₂ O₁₁	ABC转运蛋白;淀粉蔗糖代谢
Com_68_pos	0.45	甜菜碱	C₅ H₁₁ NO₂	ABC转运蛋白;甘氨酸、丝氨酸和苏氨酸代谢
Com_40_pos	0.13	L-焦谷氨酸	C₅ H₇ NO₃	谷胱甘肽代谢
Com_103_pos	-0.40	9-Oxo-ODE	C₁₈ H₃₀ O₃	亚油酸代谢
Com_144_pos	-0.51	13-HPODE	C₁₈ H₃₂ O₄	亚油酸代谢
Com_162_pos	-0.32	2-羟基肉桂酸	C₉ H₈ O₃	次级代谢生物合成;类苯基丙烷生物合成;苯丙氨酸代谢
Com_351_pos	0.51	吲哚	C₈ H₇ N	次级代谢生物合成;苯丙氨酸、酪氨酸和色氨酸生物合成;色氨酸代谢
Com_397_pos	0.59	酪氨酸	C₉ H₁₁ NO₃	次级代谢生物合成;氨基酸生物合成;类苯基丙烷生物合成;苯丙氨酸、酪氨酸和色氨酸生物合成
Com_292_pos	0.50	阿魏酸	C₁₀ H₁₀ O₄	次级代谢生物合成;类苯基丙烷生物合成
Com_421_pos	-0.54	吲哚乙酸	C₁₀ H₉ NO₂	色氨酸代谢;植物激素信号传导
Com_209_pos	-0.26	亮氨酸	C₆ H₁₃ NO₂	次级代谢生物合成;氨基酸生物合成;ABC转运蛋白;2-氧羧酸代谢;氨酰生物合成;缬氨酸、亮氨酸和异亮氨酸的生物合成;缬氨酸、亮氨酸和异亮氨酸的降解
Com_249_pos	-0.21	软脂酸	C₁₆ H₃₀ O₂	脂肪酸生物合成

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水稻DEP1突变体籽粒品质性状及其代谢组分析

Grain Quality Characters and Metabolome Analysis of DEP1 Mutant in Rice

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水稻DEP1突变体籽粒品质性状及其代谢组分析

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