摘要：

为全面系统地挖掘蔷薇科小G蛋白ROP,在分子水平上为蔷薇科抗病育种提供候选基因。物种系统进化关系分析、ROP蛋白全基因组筛查、Rho功能域分析、蛋白聚类分析及理化性质分析分别运用了NCBI、iTOLv6、GDR、SMART、MEGA 11.0和Maximum-likelihood algorithm1及Protparam和Expasy等数据库及生物信息学在线软件。11种蔷薇科植物聚为6个亚支;11种蔷薇科植物中筛查到80个ROP 蛋白;80个蔷薇科ROP蛋白和11个拟南芥ROP蛋白聚为6个进化分支;蛋白长度、等电点、分子量、脂肪指数及不稳定指数分别为143~215 aa、5.59~9.63、15.735 99~23.878 59 ku、85.28~94.95和27.96~57.14。80个蔷薇科ROP蛋白在草莓中数量最多,甜樱桃最少;蔷薇科ROP蛋白分布于第Ⅳ、Ⅴ和Ⅵ分支中,拟南芥ROP蛋白分布于Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ分支中,即分支Ⅵ为蔷薇科特异性分支,分支Ⅰ、Ⅱ和Ⅲ为拟南芥特异性分支。

关键词: 蔷薇科, 小G蛋白ROP, 全基因组筛查, Rho功能域, 系统进化关系, 蛋白理化特征

Abstract:

In order to comprehensively and systematically explore the small G protein ROP of Rosaceae, it provides candidate genes for disease resistance breeding of Rosaceae at the molecular level. Phylogenetic relationship analysis, ROP protein genome-wide screening, Rho functional domain analysis, protein cluster analysis and physicochemical property analysis used NCBI, iTOLv6, GDR, SMART, MEGA 11.0 and Maximum-likelihood algorithm1, Protparam and Expasy databases and bioinformatics online software, respectively. Eleven species of Rosaceae were clustered into 6 subclades; 80 ROP proteins were screened in 11 species of Rosaceae; 80 Rosaceae ROP proteins and 11 Arabidopsis ROP proteins were aggregated into 6 evolutionary branches; the protein length, isoelectric point, molecular weight, fat index and instability index were 143—215 aa, 5.59—9.63, 15.735 99—23.878 59 ku,85.28—94.95 and 27.96—57.14, respectively. 80 Rosaceae ROP proteins were the most abundant in Fragaria ananassa and the least in Prunus avium; Rosaceae ROP proteins were distributed in branches Ⅳ, Ⅴ and Ⅵ, Arabidopsis ROP proteins were distributed in branches Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ, that is, branch Ⅵ was a Rosaceae-specific branch, and branches Ⅰ, Ⅱ and Ⅲ were Arabidopsis-specific branches.

Key words: Rosaceae, Small G protein ROP, Genome-wide screening, Rho functional domain, Phylogenetic relationship, Physicochemical characteristics of protein