二苯基氯化碘盐及过氧化氢预处理对缺钾环境下甘薯幼苗抗氧化系统的影响

doi:10.7668/hbnxb.20194278

摘要/Abstract

摘要：

为了研究缺钾胁迫下活性氧(ROS)对甘薯生长和抗氧化系统的影响,进一步揭示活性氧调控甘薯缺钾胁迫下的抵御机制。选用低钾耐受型品种徐薯32和低钾敏感型品种宁紫薯1号2种甘薯品种为试验材料,通过人工气候室内水培的方式确保试验过程中环境因素保持一致,采用外源H₂O₂和二苯基氯化碘ROS抑制剂(DPI)预处理甘薯幼苗之后再进行缺钾处理,检测缺钾处理后不同时间点下甘薯根系内源H₂O₂相对含量、抗氧化基因表达水平及酶活力指标的变化,记录甘薯的形态特征,探索ROS信号对缺钾胁迫下甘薯苗期生长的影响。研究结果表明,缺钾处理下,甘薯生物量降低,叶色变淡;甘薯幼苗根系ROS相对浓度在第14天荧光强度表现最高;甘薯根系中抗氧化相关基因的表达量普遍升高,H₂O₂预处理表达量升高更显著;与-K处理相比,H₂O₂和DPI预处理均可提高甘薯叶片中的抗氧化酶活性,不同种酶活性在缺钾处理21 d内的变化趋势有所差异;不同处理的影响效应存在品种差异。H₂O₂可作为胁迫信号分子,提高甘薯幼苗抗氧化基因表达水平,提高抗坏血酸酶(APX)、超氧化物歧化酶(SOD)和过氧化物酶(POD)的酶活,从而清除多余ROS,有效缓解缺钾胁迫对甘薯幼苗生长的抑制,而DPI可以抑制内源H₂O₂的产生,维持抗氧化酶活,保护甘薯幼苗ROS酶促清除系统,避免组织的氧化损伤。

关键词: 甘薯, 钾胁迫, H₂O₂, 活性氧, 二苯基氯化碘盐

Abstract:

In order to investigate the effects of reactive oxygen species(ROS)on the growth and antioxidant system of sweetpotato under potassium deficiency stress,and further reveal the defense mechanism of ROS regulation in sweetpotato under potassium deficiency stress.This study selected two sweetpotato varieties,Xushu32(low potassium tolerant)and Ningzi 1(low potassium sensitive),as experimental materials.The experiment was conducted in an artificial climate chamber using hydroponic cultivation to ensure consistent environmental conditions.After pretreatment with exogenous H₂O₂ and diphenyl chloroiodate(DPI),ROS inhibitor,the sweetpotato seedlings were subjected to potassium deficiency treatment.The changes in relative H₂O₂ content,antioxidant gene expression levels,and enzyme activity indicators in sweetpotato roots at different time points after potassium deficiency treatment were measured.The morphological characteristics of sweetpotato were recorded to explore the effects of ROS signaling on the growth of sweetpotato seedlings under potassium deficiency stress.The results showed that under potassium deficiency treatment,the biomass of sweetpotato decreased and leaf color lightened.The relative concentration of ROS in sweetpotato seedling roots showed the highest fluorescence intensity on the 14th day.The expression levels of antioxidant-related genes in sweetpotato roots generally increased,with a more significant increase in expression levels after H₂O₂ pretreatment.Compared with-K treatment,both H₂O₂ and DPI pretreatment could enhance the activity of antioxidant enzymes in sweetpotato leaves,and different enzyme activities showed different trends within 21 days of potassium deficiency treatment.There were varietal differences in the effects of different treatments.The experimental results showed that H₂O₂ could act as a stress signaling molecule to increase the expression level of antioxidant genes and the enzymatic activities of APX,SOD and POD in sweetpotato seedlings,thus scavenging excess ROS and effectively alleviating the inhibition of growth of sweetpotato seedlings by potassium deficiency stress.And DPI could inhibit the production of endogenous H₂O₂,maintain the antioxidant enzyme activity,protect the ROS enzymatic scavenging system of sweetpotato seedlings and avoid oxidative damage to tissues.

Key words: Sweetpotato, Potassium deficiency stress, H₂O₂, ROS, DPI

郭鹏宇, 张强强, 靳容, 赵鹏, 王静, 朱晓亚, 于永超, 刘明, 吴德祥, 唐忠厚. 二苯基氯化碘盐及过氧化氢预处理对缺钾环境下甘薯幼苗抗氧化系统的影响[J]. 华北农学报, 2024, 39(S1): 108-116. doi: 10.7668/hbnxb.20194278.

GUO Pengyu, ZHANG Qiangqiang, JIN Rong, ZHAO Peng, WANG Jing, ZHU Xiaoya, YU Yongchao, LIU Ming, WU Dexiang, TANG Zhonghou. Effect of Pretreatment with DPI and H₂O₂ on the Antioxidant System of Sweetpotato Seedlings in a Potassium Deficient Environment[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 108-116. doi: 10.7668/hbnxb.20194278.

http://www.hbnxb.net/CN/Y2024/V39/IS1/108

图/表 6

表1 本研究中用到的引物

Tab.1 Primers used in this study

基因名 Gene name	引物 Primers	序列(5'-3') Sequence (5'-3')	碱基数 Bases
IbARF	IbARF-F	CTTTGCCAAGAAGGAGATGC	20
	IbARF-R	TCTTGTCCTGACCACCAACA	20
IbAPX1	IbAPX1-F	TGGCACTCTGCTGGAACT	18
	IbAPX1-R	GCATAAGAAACAATAGGGA	19
IbCuZnSOD	IbCuZnSOD-F	GGGTGTCGCAGTTCTTAG	18
	IbCuZnSOD-R	ATTGAAATGTGGTCCAGTAG	20
IbPOD	IbPOD-F	TCTTCTTCCACGATTGCTT	19
	IbPOD-R	AGTCACAGTGTATGTTGCTCC	21
IbRbohF	IbRbohF-F	TTGGAAAGAGTGGGTTGC	18
	IbRbohF-R	CAGGATACTAATAAAGGGTGT	21

图1 不同处理21 d后Xu32和Nz1的表型差异

Fig.1 Phenotypic differences of Xu32 and Nz1 under different treatments at 21 d

图2 21 d内不同处理间对Xu32和Nz1根系ROS相对含量定性测定

Fig.2 Qualitative determination of the relative ROS content of Xu32 and Nz1 roots among different treatments in 21 d

图3 21 d内不同处理间对Xu32和Nz1根系ROS相对含量定量测定柱上的不同小写字母表示同一时间内处理间在P<0.05水平差异显著。图4-5同。

Fig.3 Quantitative determination of the relative ROS content of Xu32 and Nz1 roots among different treatments in 21 d For the same time,different small letters above bars indicate significant difference among treatments at P<0.05.The same as Fig.4-5.

图4 21 d内不同处理对Xu32和Nz1根系抗氧化基因RT-PCR表达的影响

Fig.4 Effect of different treatments on RT-PCR expression of antioxidant-related genes in Xu32 and Nz1 roots during 21 d

图5 21 d内不同处理对Xu32和Nz1叶片抗氧化酶活性的影响

Fig.5 Effects of different treatments on the activities of antioxidant-related enzymes in Xu32 and Nz1 roots during 21 d

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