华北农学报 ›› 2024, Vol. 39 ›› Issue (5): 93-101. doi: 10.7668/hbnxb.20194759

所属专题: 棉花 盐碱胁迫 热点论文 栽培生理

• 耕作栽培·生理生化 • 上一篇    下一篇

外源H2O2对盐胁迫下棉花幼苗生理特性的影响

肖陈耀东, 刘涛, 刘仕志, 张淑英   

  1. 石河子大学 农学院,新疆 石河子 832003
  • 收稿日期:2024-03-12 出版日期:2024-10-28
  • 通讯作者:
    张淑英(1976-),女,安徽泗县人,副教授,博士,硕士生导师,主要从事植物营养及植物生理研究。
  • 作者简介:

    肖陈耀东(1998-),男,新疆库尔勒人,在读硕士,主要从事作物逆境生理研究。

  • 基金资助:
    国家自然科学基金项目(42067021)

Effect of Exogenous Hydrogen Peroxide on the Physiological Characteristics of Cotton Seedlings under Salt Stress

XIAO Chenyaodong, LIU Tao, LIU Shizhi, ZHANG Shuying   

  1. Agricultural College,Shihezi University,Shihezi 832003,China
  • Received:2024-03-12 Published:2024-10-28

摘要:

为探明外源H2O2对NaCl胁迫下棉花幼苗的生理调节机制,以棉花品种新陆早48号为供试材料,使用室外盆栽法,设置盐胁迫(NaCl,浓度梯度0,100,200 mmol/L) 和H2O2(浓度梯度0,0.005,0.010,0.020,0.050 mmol/L) 两因素随机组合,探究棉花幼苗鲜质量、干质量、叶绿素含量、叶绿素荧光参数、光合气体参数、抗氧化酶活性和渗透调节系统的变化规律。结果表明,外源H2O2有效缓解盐胁迫对棉花幼苗生长的抑制效应,提高棉花幼苗叶绿素含量、光合气体参数、叶绿素荧光参数,维持棉花幼苗光合作用正常运行,保证了干物质的积累;同时,外源H2O2提高抗氧化酶(POD、APX、CAT)活性,加速清除棉花幼苗体内ROS,降低了电解质渗出率、MDA含量及Pro、游离氨基酸、SS等渗透调节物质含量,提高棉苗抗盐性。其中0.020 mmol/L外源H2O2对棉花幼苗所受盐胁迫缓解作用最佳。综上,外源H2O2通过提高盐胁迫棉花幼苗光合性能,保持棉花幼苗稳定的光合作用,维持棉花幼苗体内ROS产生与消除的动态平衡,从而提高棉花幼苗对盐胁迫的适应性。

关键词: 棉花幼苗, 盐胁迫, 外源H2O2, 光合特性, 抗氧化酶系

Abstract:

In order to investigate the physiological regulation mechanism of exogenous H2O2 on the cotton seedlings under NaCl stress,the cotton variety Xinluzao 48 was used as the test material in an outdoor potting method.Two-factor random combinations of salt stress (NaCl,concentration gradients of 0,100,200 mmol/L) and H2O2 (concentration gradients of 0,0.005,0.010,0.020,0.050 mmol/L) were set,to study the change rule of fresh weight,dry weight,chlorophyll content,chlorophyll fluorescence parameters,photosynthetic gas parameters,antioxidant enzyme activities and osmotic regulation system of cotton seedlings.The results showed that exogenous H2O2 effectively alleviated the inhibitory effect of salt stress on the growth of cotton seedlings,increased chlorophyll content,photosynthetic gas parameters and chlorophyll fluorescence parameters of cotton seedlings,maintained the normal operation of photosynthesis of cotton seedlings and ensured the accumulation of dry matter.Meanwhile,exogenous H2O2 could increase the activity of antioxidant enzymes (POD,APX,CAT),accelerated the removal of ROS from cotton seedlings.Exogenous H2O2 reduced the electrolyte leakage rate,MDA content,the content of osmoregulation substances such as pro,free amino acid and SS,and improved the salt resistance of cotton seedlings.Among all treatment,0.020 mmol/L exogenous H2O2 had the best effect in alleviating the salt stress suffered by cotton seedlings.In summary,exogenous H2O2 improves the adaptation of cotton seedlings to salt stress by improving photosynthetic performance,keeping stable photosynthesis in cotton seedlings,and maintaining the dynamic balance between ROS production and elimination in cotton seedlings.

Key words: Cotton seedlings, Salt stress, Exogenous H2O2, Photosynthetic performance, Antioxidant enzyme system

引用本文

肖陈耀东, 刘涛, 刘仕志, 张淑英. 外源H2O2对盐胁迫下棉花幼苗生理特性的影响[J]. 华北农学报, 2024, 39(5): 93-101. doi: 10.7668/hbnxb.20194759.

XIAO Chenyaodong, LIU Tao, LIU Shizhi, ZHANG Shuying. Effect of Exogenous Hydrogen Peroxide on the Physiological Characteristics of Cotton Seedlings under Salt Stress[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 93-101. doi: 10.7668/hbnxb.20194759.