抗(感)谷瘟病谷子品种内生菌多样性分析

doi:10.7668/hbnxb.20192900

摘要/Abstract

摘要：

为了揭示不同品种以及不同器官中内生菌的多样性特征,初步明确内生菌群落结构与宿主品种及器官类型的相关性。以感谷瘟病品种沙湾谷子、冀谷22和抗谷瘟病品种小青谷、石榴子为材料,分别取植株的茎、叶片、叶鞘,通过基于16S rDNA V3—V4区的高通量测序进行微生物多样性分析。结果显示,感病品种与抗病品种内生菌物种组成具有一定差异性,所用供试样本中,门水平优势类群均为变形菌门、放线菌门,拟杆菌门、绿弯菌门、粘菌门、厚壁菌门次之。Alpha多样性分析表明,感病品种(沙湾谷子、冀谷22)叶片内生菌丰富度较高;PCoA分析则表明,器官类型比品种对内生菌群落结构影响更大。物种组成分析表明,感病品种沙湾谷子及冀谷22含有与小青谷、石榴子(抗谷瘟病)差异较大的内生菌群,感谷瘟病品种(沙湾谷子、冀谷22)叶片都具有Entotheonellaeota;抗谷瘟病品种则在叶鞘中均具Hydrogenedentes。明确了感、抗谷瘟病品种谷子及各个品种的不同器官内生菌的多样性及群落结构具有一定差异,器官类型比品种对内生菌群落结构的影响更大。

关键词: 谷子, 谷瘟病, 内生菌, 多样性

Abstract:

In order to reveal the diversity characteristics of endophytes in different species and different organs,the correlation between endophyte community structure and host species and organ types was preliminarily clarified.The stems,leaves and leaf sheaths,which were respectively collected from the plants of the foxtail millet blast-susceptible varieties Shawan millet and Jigu 22 and the foxtail millet blast resistant varieties Xiaoqinggu and Shiliuzi,were used to carry out the endophytic diversity by high-throughput sequencing based on the 16S rDNA V3—V4 region.There were certain differences in endophyte species composition between susceptible and resistant varieties.In all tested samples,the dominant groups at the phylum level were Proteobacteria and Actinobacteriota.Bacteroidota,Chloroflexi,Myxococcota,Firmicutes followed.Alpha diversity analysis showed that the susceptible varieties(Shawan millet,Jigu 22)had higher abundance of endophyte in leaves.PCoA analysis revealed that the organ type had a greater impact on the endophyte community structure than the variety.Species composition analysis showed that the susceptible varieties Shawan millet and Jigu 22 contained endophytic flora that were significantly different from those of Xiaoqinggu and pomegranate(resistant to foxtail millet blast).The susceptible varieties(Shawan millet,Jigu 22)contained Entotheonellaeota phylum in leaves,while resistant varieties had Hydrogenedentes phylum in leaf sheaths.It clarified that the diversity and community structure of endophytes in different organs and millet varieties susceptible and resistant to foxtail millet blast were different,and organ types had a greater impact on the community structure of endophytes than varieties.

Key words: Setaria italica, Magnaporthe oryzae, Endogenic bacteria, Diversity

刘佳悦, 贾丽霞, 王苗苗, 孙东辉, 孙贺贺, 郝志敏, 李志勇. 抗(感)谷瘟病谷子品种内生菌多样性分析[J]. 华北农学报, 2022, 37(5): 187-193. doi: 10.7668/hbnxb.20192900.

LIU Jiayue, JIA Lixia, WANG Miaomiao, SUN Donghui, SUN Hehe, HAO Zhimin, LI Zhiyong. Analysis of Endophytic Diversity of Foxtail Millet Varieties Resistant(Susceptible)to Foxtail Millet Blast[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 187-193. doi: 10.7668/hbnxb.20192900.

http://www.hbnxb.net/CN/Y2022/V37/I5/187

图/表 7

表1 不同品种谷子取样信息

Tab.1 Sampling information of different foxtail millet varieties

品种 Variety	抗病性 Disease resistance	部位 Organ
沙湾谷子 Shawan millet	感谷瘟病 S	叶片、茎、叶鞘
冀谷22 Jigu 22	感谷瘟病 S	叶片、茎、叶鞘
小青谷 Xiaoqinggu	抗谷瘟病 R	叶片、茎、叶鞘
石榴子 Shiliuzi	抗谷瘟病 R	叶片、茎、叶鞘

图1 不同品种谷子内生菌分布 G1-1.沙湾谷子叶片;G3-1.沙湾谷子茎;G4-1.沙湾谷子叶鞘;G1-2.冀谷22叶片;G3-2.冀谷22茎;G4-2.冀谷22叶鞘;G1-4.小青谷叶片;G3-4.小青谷茎;G4-4.小青谷叶鞘;G1-5.石榴子叶片;G3-5.石榴子茎;G4-5.石榴子叶鞘。

Fig.1 Distribution of endophytes in different varieties of foxtail millet G1-1.Shawan millet leaves;G3-1.Shawan millet stem;G4-1.Shawan millet leaf sheath;G1-2.Jigu 22 leaves;G3-2.Jigu 22 stem;G4-2.Jigu 22 leaf sheath;G1-4.Xiaoqinggu leaves;G3-4.Xiaoqinggu stem;G4-4.Xiaoqinggu leaf sheath;G1-5.Shiliuzi leaves;G3-5.Shiliuzi stem;G4-5.Shiliuzi leaf sheath.

图2 不同品种谷子内生菌稀释曲线 A~D.沙湾谷子、冀谷22号、小青谷、石榴子。图4~5同。

Fig.2 Dilution curve of foxtail millet endophytes in different varieties A—D.Shawan millet,Jigu 22,Xiaoqinggu,Shiliuzi.The same as Fig.4—5.

图3 谷子Alpha多样性指数分析 A~C.叶片、茎、叶鞘;G-1.沙湾谷子;G-2.冀谷22号;G-4.小青谷;G-5.石榴子。

Fig.3 Analysis of Alpha diversity index of foxtail millet A—C.Leaves,stems,leaf sheath;G-1.Shawan millet;G-2.Jigu 22;G-4.Xiaoqinggu;G-5.Shiliuzi.

图4 不同品种谷子内生菌PCoA分析 G1.叶片;G3.茎;G4.叶鞘。

Fig.4 PCoA analysis of endophytes of foxtail millet in different varieties G1.Leaves;G3.Stems;G4.Leaf sheath.

图5 OTU分布的Veen图分析

Fig.5 Veen plot analysis of the OTU distribution

图6 谷子不同部位OTU分布的Veen图分析 A~C.谷子的叶片、茎、叶鞘。

Fig.6 Veen plot analysis of OTU distribution in different parts of foxtail millet A—C.Leaves,stem and leaf sheath of foxtail millet.

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