陕西省地方小麦品种黄色素含量基因的遗传分析

doi:10.7668/hbnxb.2010.04.018

摘要/Abstract

摘要： 面粉及其制成品的色泽是衡量小麦品质的重要指标之一.八氢番茄红素合成酶(Phytoene synthase,Psy)基因是影响小麦黄色素含量的关键基因,利用分子标记检测 98 份陕西省地方小麦品种(系)Psy-A1与 Psy-B1基因的等位变异及其分布,进一步验证Psy-A1与Psy-B1基因分子标记的有效性.利用黄色素含量基因(Psy-A1、Psy-B1) 的功能标记 YP7A 和 YP7B 检测了 98 份陕西省1980年以来自育和引进的小麦品种(系)Psy-A1等位基因Psy-A1a (片段长度为 194 bp,高黄色素含量)、Psy-A1b(片段长度为231 bp,低黄色素含量)和Psy-B1等位基因Psy-B1a (片段长度为11 bp,中黄色素含量)、Psy-B1b (片段长度为16 bp,低黄色素含量)、Psy-B1c (片段长度为428 bp,高黄色素含量) 与 Psy-B1d (片段长度为884 bp,不确定与黄色素含量的关系)的分布情况,并探讨了Psy-A1、Psy-B1等位基因在品种间的遗传关系.结果表明,陕西省主栽小麦品种(系)中Psy-A1a和Psy-A1b 基因型的频率分别为39.8%和60.2%,以低黄色素含量的Psy-A1b基因型为主;Psy-B1a、Psy-B1b、Psy-B1c与Psy-B1d 四种基因型的分布频率分别是24.%,67.4%,7.1%和1.0%.对属于黄淮冬麦区之陕西汾渭河谷副区的小麦品种(系)Psy-A1a、Psy-A1b、Psy-B1a、Psy-B1b、Psy-B1c和Psy-B1d 基因型的分布频率分别为4.7%、4.3%,17.4%,71.7%,10.9%和0%;对属于西南冬麦区之陕南鄂西丘陵副区的小麦品种(系)Psy-A1a、Psy-A1b、Psy-B1a、Psy-B1b、Psy-B1c和Psy-B1d 基因型的分布频率则分别为32.7%,67.3%,30.8%,63.%,3.9%和1.9%,说明不同副麦区的小麦品种(系)基因型差异明显,同时可以直接利用黄色素含量基因(Psy-A1和Psy-B1 )的功能标记YP7A和YP7B来提高小麦育种效率.

关键词: 普通小麦, 黄色素, 八氢番茄红素合成酶基因, 功能标记, 分子标记辅助选择

Abstract: Color is an important trait exerting significant influences on the appearance of wheat－based products. Phytoene synthase (Psy) gene is a critical gene influencing biosynthetic pathway of yellow pigment (YP) in bread wheat. The aims of this study were to detect the allelic variations of Psy-A1 and Psy-B1 genes in 98 wheat cultivars and lines grown mainly in Shaanxi province using the functional markers YP7A and YP7B developed from the sequence polymorphisms of Psy-A1 gene on chromosome 7A and Psy-B1 gene on chromosome 7B，and to identify the distribution and to discuss the relationship between allelic variations of Psy-A1 and Psy-B1 genes to evaluate the feasibility of the markers for selecting wheat cultivars and lines with either low or high YP content. YP7A. The co-dominant marker of Psy-A1 gene can amplify a 194-bp and a 231-bp fragment in the genotypes with Psy-A1a frequently for high YP content and Psy-A1b mostly for low YP content，r espectively; and YP7B-1，another co-dominant marker of Psy-B1 gene，can amplify a 151-bp PCR fragment in accessions with the medium YP content allele Psy-B1a，and a 156-bp fragment in lower YP content accessions with Psy-B1b; two dominant markers YP7B-2 (428 bp) and YP7B-3 (884 bp) were designed for accessions with Psy-B1c and Psy-B1d，respectively. Allele Psy-B1c was associated with high YP content，but the phenotypic effect of Psy-B1d was not determined due to the limited number of accessions. The detecting results showed that the frequency of the genotype Psy-A1a，Psy-A1b，Psy-B1a，Psy-B1b，Psy-B1c and Psy-B1d is 39. 8%， 60. 2%，24. 5%， 67. 4%，7 . 1% and 1. 0%，respectively. In summary ，cultivars and lines with genotypes Psy- A1b and Psy-B1b should be mainly selected for the improvement and introduction of flour color in Shaanxi wheat cultivars (lines) . The functional markers YP7A and YP7B of phytoene synthase genes ( Psy-A1 and Psy-B1) can be directly used for promoting the breeding efficiency.

Key words: Bread wheat (Triticum aestivum L.), Yellow pigment(YP), Phytoene synthase (Psy) gene, Functional marker, Marker-assisted selection (MAS)

中图分类号:

S12.03

杨松杰, 张影全, 王茹, 张晓科. 陕西省地方小麦品种黄色素含量基因的遗传分析[J]. 华北农学报, 2010, 25(4): 83-87. doi: 10.7668/hbnxb.2010.04.018.

YANG Song-jie, ZHANG Ying-quan, WANG Ru, ZHANG Xiao-ke. Genetic Analysis of Genes for Yellow Pigment Content in Shaanxi Wheat Cultivars and Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 83-87. doi: 10.7668/hbnxb.2010.04.018.

http://www.hbnxb.net/CN/Y2010/V25/I4/83

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