玉米第10染色体大刍草单片段代换系的构建及穗长QTL分析

doi:10.7668/hbnxb.2018.02.015

摘要/Abstract

摘要： 为了构建玉米第10染色体大刍草单片段代换系群体并进行穗长 QTL的筛选。以玉米野生近缘种墨西哥类玉米为供体亲本，以玉米自交系郑58为受体亲本和轮回亲本连续进行多代回交。利用BC₇F₁至BC₉F₁连续回交群体，选用45对在供体和受体亲本间有明显多态性差异的引物进行SSR分子标记辅助选择，构建以墨西哥类玉米为供体亲本的玉米第10染色体单片段导入系群体。对穗长性状QTL在第10染色体上位置进行初步定位。经过连续多代回交和SSR标记跟踪检测，获得了以郑58为遗传背景的墨西哥类玉米导入系群体，共检测到单片段代换系材料107份，代换片段平均长度为25.52~45.97 cM，总长度为944.15~1 884.74 cM，对第10染色体覆盖率为70.38%~89.65%。在BC₉F₁回交群体中鉴定了4个来自墨西哥类玉米并位于第10染色体的穗长QTL，初步定位于umc2528、phi054、bnlg1360和umc1877标记附近。结果表明，利用大刍草作为供体亲本可以获得大量玉米第10染色体单片段代换系材料，为优良QTL性状的发掘和筛选奠定了材料基础，拓宽了玉米新品种选育的种质资源范围。

关键词: 玉米, 单片段代换系, 分子标记辅助选择, QTL

Abstract: The purpose of this study is to construct maize SSSLs of teosinte on the tenth chromosome and select the spike length QTLs. Wild relatives specie of annual Mexican corn was selected as donor parent,maize inbred lines Zheng 58 was selected as recipient parent and recurrent parent for continuous multi-generation backcross. Forty-five pairs of primers showed obvious polymorphism difference between donor and recipient parents were choiced for SSR molecular marker-assisted selection. The successive backcross populations from BC₇F₁ to BC₉F₁ were used as detection materials,constructing single segment substitution lines populations of the tenth chromosome with Mexican corn as donor parent. At the same time,the loci of spike length QTLs on the tenth chromosome were preliminarily mapped.By studying,107 single segment substitution materials from Mexican corn introgression lines were obtained by continuous multi-generation backcrossing and SSR molecular marker detecting,using Zheng 58 as recipient parent. The average length of SSSLs was 25.52-45.97 cM,the total length of SSSLs was 944.15-1 884.74 cM,the coverage rate of SSSLs on the tenth chromosome was 70.38% -89.65%. Four spike length QTLs were identified in BC₉F₁ population,which came from donor parent and located on the tenth chromosome,the QTLs were preliminarily mapped nearby umc2528,phi054,bnlg1360 and umc1877 markers. The results indicates that many maize SSSLs of the tenth chromosome are constructed utilizing the wild gene pool of teosinte,which establishes the material foundation for excavating and selecting of the desirable QTL traits,and have broadened the range of germplasm resources on new varieties breeding of maize.

Key words: Maize, Single segment substitution lines, Molecular marker-assisted selection, Quantitative trait loci

中图分类号:

S326

江院, 王启亮, 解新明, 卢小良, 张向前. 玉米第10染色体大刍草单片段代换系的构建及穗长QTL分析[J]. 华北农学报, 2018, 33(2): 100-111. doi: 10.7668/hbnxb.2018.02.015.

JIANG Yuan, WANG Qiliang, XIE Xinming, LU Xiaoliang, ZHANG Xiangqian. Construction of Teosinte Single Segment Substitution Lines and Spike Length QTL Analysis on the Tenth Chromosome of Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(2): 100-111. doi: 10.7668/hbnxb.2018.02.015.

http://www.hbnxb.net/CN/Y2018/V33/I2/100

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