摘要： 为了研究不同钾素浓度对钾转运体KUP6和KUP7基因表达量的影响，通过水培法培养马铃薯幼苗，通过提取马铃薯总RNA，经检测合格后用反转录试剂盒合成其cDNA的第一链。运用实时荧光定量核酸扩增检测技术对幼苗叶片进行基因转录水平的检测。在马铃薯幼苗种植后的第10，20，30 天分别测定马铃薯幼苗根、茎和叶中钾的含量，并进行统计分析。结果表明：钾素浓度为1.00 mmol/L时，KUP6和KUP7基因表达量最高，且与其他处理有显著差异；钾素浓度低于0.10 mmol/L时，表达量较低，钾素浓度为10.00 mmol/L时该基因表达量也降低。表明较高和较低的钾离子浓度都不利于KUP6和KUP7基因的表达。通过对根、茎和叶内钾素含量测定表明：对于根系来说，钾素浓度在1.0 mmol/L时已到达其吸收饱和值；在钾素浓度为1.00，10.00 mmol/L的高钾水培中，马铃薯根、茎和叶钾素含量大于水培钾素浓度为0.01，0.10 mmol/L的低钾处理；在低钾水培条件下，茎叶随着培养时间的延长，其钾素含量之间的差异逐渐减小，而在高钾条件下，其差异逐渐增大。

关键词: 马铃薯, 钾转运体, qPCR, KUP6, KUP7

Abstract: In order to study response of the potassium concentration on the expression of KUP6 and KUP7 potassium transporter gene,hydroponic potato seedlings was conducted.The RNA of potato was isolated and checked, the first chain of the cDNA was synthesized by the Reverse Transcriptional Kit, and gene transcription on seedlings was detected by Real-time fluorescence quantitative PCR technique and the content of potassium in potato seedlings was detected.The content of potassium in potato seedlings was detected at 10, 20 and 30 days after potato seedling planting. The results showed that when potassium concentration was 1.00 mmol/L,the expression level of KUP6 and KUP7 was the highest. When potassium concentration was lower than 0.10 mmol/L,the expression was low,and the expression level of potassium decreased when potassium concentration was 10 mmol/L. It indicates that higher and lower potassium concentration is not conducive to the expression of KUP6 and KUP7 genes. Potassium content in roots, stems and leaves showed that potassium concentration reached its absorption saturation at 1.00 mmol/L for root. The higher potassium concentration in hydroponics, the higher potassium content in root, stem and leaf. The difference of potassium content in stem and leaf was decreased when lower potassium concentration in hydroponics and increased when higher concentration.

Key words: Potato, Potassium transporter, qPCR, KUP6, KUP7

中图分类号: 

• S141.3

• Q946